Antioxidant and Anticlastogenic Capacity of Prickly Pear Juice
Abstract
:1. Introduction
2. Experimental Section
2.1. Chemicals
2.2. Animals
2.3. Experimental Determinations
2.3.1. Obtaining and Preparation of Three Varieties of Prickly Pear Juice
2.3.2. DPPH Radical Scavenging Activity
2.3.3. Genotoxicity/Antigenotoxicity Protocols
2.3.4. Statistical Analysis
3. Results
4. Discussion
5. Conclusions
Conflicts of Interest
References
- Sumaya-Martínez, M.T.; Cruz-Jaime, S.; Madrigal-Santillán, E.; García-Paredes, D.; Cariño-Cortés, R.; Cruz-Cansino, N.; Valadez-Vega, C.; Martínez-Cárdenas, L.; Alanís-García, E. Betalain, acid ascorbic, phenolic contents and antioxidant properties of purple, red, yellow and white cactus pears. Int. J. Mol. Sci. 2011, 12, 6452–6468. [Google Scholar] [CrossRef]
- Stintzing, F.C.; Schieber, A.; Carle, R. Phytochemical and nutritional significance of cactus pear. Eur. Food Res. Technol. 2001, 212, 396–407. [Google Scholar] [CrossRef]
- Madrigal-Santillán, E.; Madrigal-Bujaidar, E.; Cruz-Jaime, S.; Valadez-Vega, M.; Sumaya-Martínez, M.T.; Pérez-Ávila, K.; Morales-González, J.A. The Chemoprevention of Chronic Degenerative Disease Through Dietary Antioxidants: Progress, Promise and Evidences. In Oxidative Stress and Chronic Degenerative Diseases—A Role for Antioxidants; Morales-González, J.A., Ed.; InTech: Rijeka, Croatia, 2013; pp. 155–186. [Google Scholar]
- Hegwood, D.A. Human health discoveries with Opuntia sp. (prickly pear). HortScience 1990, 25, 1515–1516. [Google Scholar]
- Livrea, M.A.; Tesoriere, L. Antioxidant Activities of Prickly Pear (Opuntia ficus indica) Fruit and Its Betalains, Betanin and Indicaxanthin. In Herbal and Traditional Medicine: Molecular Aspects of Health; Packer, L., Nam, O.C., Halliwell, B., Eds.; Marcel Dekker: New York, NY, USA, 2004; pp. 537–556. [Google Scholar]
- Ibanez-Camacho, R.; Meckes-Lozoya, M.; Mellado-Campos, V. The hypoglycemic effect of Opuntia streptacantha studied in different animal experimental models. J. Ethnopharmacol. 1983, 7, 175–181. [Google Scholar] [CrossRef]
- Trejo-Gonzales, A.; Gabriel-Ortiz, G.; Puebla-Perez, A.M.; Huizar-Contreras, M.D.; Munguia-Mazariegos, M.R.; Mejia-Arreguin, S.; Calva, E. A purified extract from prickly pear cactus (Opuntia fuliginosa) controls experimentally induced diabetes in rats. J. Ethnopharmacol. 1996, 55, 27–33. [Google Scholar] [CrossRef]
- Fernández, M.L.; Lin, E.C.K.; Trejo, A.; McNamara, D.J. Prickly pear (Opuntia sp.) pectin reverse low density lipoprotein receptor suppression induced by a hypercholesterolemic diet in guinea pigs. J. Nutr. 1992, 22, 2230–2340. [Google Scholar]
- Munoz-de-Chavez, M.; Chavez, A.; Valles, V.; Roldan, J.A. The nopal: A plant of manifold qualities. World Rev. Nutr. Diet. 1995, 77, 109–134. [Google Scholar]
- Sawaya, W.N.; Khatchadourian, H.A.; Safi, W.M.; Al-Hammad, H.M. Chemical characterization of prickly pear pulp, Opuntia ficus-indica, and the manufacturing of prickly pear jam. J. Food Technol. 1983, 18, 183–193. [Google Scholar]
- Kuti, J.O. Antioxidant compounds from four Opuntia cactus pear fruit varieties. Food Chem. 2004, 85, 527–533. [Google Scholar] [CrossRef]
- Fernández-López, J.; Almela, L.; Obón, J.; Castellar, R. Determination of antioxidant constituents in cactus pear fruits. Plant Foods Hum. Nutr. 2010, 65, 253–259. [Google Scholar] [CrossRef]
- Castellar, R.; Obón, J.M.; Alacid, M.; Fernández-López, J.A. Color properties and stability of betacyanins from Opuntia fruits. J. Agric. Food Chem. 2003, 51, 2772–2776. [Google Scholar] [CrossRef]
- Livrea, M.A.; Tesoriere, L. Antioxidative effects of cactus pear (Opuntia ficus-indica (L.)) Mill. Fruits from Sicily and bioavailability of betalain components in healthy humans. Acta Hortic. 2009, 811, 197–204. [Google Scholar]
- Siriwardhana, N.; Shahidi, F.; Jeon, Y.J. Potential antioxidative effects of cactus pear fruit (Opuntia ficus-indica) extract on radical scavenging and DNA damage reduction in human peripheral lymphocytes. J. Food Lipids 2006, 13, 445–458. [Google Scholar] [CrossRef]
- Zorgui, L.; Ayed-Boussema, I.; Ayed, Y.; Bacha, H.; Hassen, W. The antigenotoxic activities of cactus (Opuntia ficus-indica) cladodes against the mycotoxin zearalenone in Balb/c mice: Prevention of micronuclei, chromosome aberrations and DNA fragmentation. Food Chem. Toxicol. 2009, 47, 662–667. [Google Scholar] [CrossRef]
- Burtis, M.; Bucar, F. Antioxidant activity of Nigella sativa essential oil. Phytother. Res. 2000, 14, 323–328. [Google Scholar] [CrossRef]
- Madrigal-Santillán, E.; Alvarez-González, I.; Márquez-Márquez, R.; Velázquez-Guadarrama, N.; Madrigal-Bujaidar, E. Inhibitory effect of mannan on the toxicity produced in mice fed aflatoxin B1 contaminated corn. Arch. Environ. Contam.Toxicol. 2007, 53, 466–472. [Google Scholar] [CrossRef]
- Kalra, E.K. Nutraceutical-definition and introduction. AAPS PharmSci. 2003, 5, 27–28. [Google Scholar] [CrossRef]
- Piga, A. Cactus pear: A fruit of nutraceutical and functional. J. Prof. Assoc. Cactus Dev. 2004, 6, 9–22. [Google Scholar]
- Stintzing, F.C.; Schieber, A.; Carle, R. Cactus pear—A promising component to functional food. Obst. Gem. Kartoffelverarbeitung 2000, 86, 40–47. [Google Scholar]
- Park, E.H.; Kahng, H.; Lee, S.H.; Shin, K.H. An anti-inflammatory principle from cactus. Fitoterapia 2001, 72, 288–290. [Google Scholar] [CrossRef]
- Zou, D.M.; Brewer, M.; Garcia, F.; Feugang, J.M.; Wang, J.; Zang, R.; Lieu, H.; Zou, C.P. Cactus pear—A natural product in cancer chemopretection. Nutr. J. 2005, 4, 25. [Google Scholar] [CrossRef]
- Tesoriere, L.; Butera, D.; Pintaudi, M.; Allegra, M.; Livera, M.A. Supplementation with cactus pear (Opuntia ficus-indica) fruit decreases oxidative stress in healthy humans: A comparative study with Vitamin C. Am. J. Clin. Nutr. 2004, 80, 391–395. [Google Scholar]
- Stintzing, F.C.; Carle, R. Cactus stems (Opuntia spp.): A review on their chemistry, technology, and uses. Mol. Nutr. Food Res. 2005, 49, 175–194. [Google Scholar] [CrossRef]
- Abdel-Nabey, A.A. Chemical and technological studies on prickly pear (Opuntia ficus indica) fruits. Alex. J. Agric. Res. 2001, 46, 61–70. [Google Scholar]
- Antolovich, M.; Prenzler, P.D.; Patsalides, E.; Mcdonald, S.; Robards, K. Methods for testing antioxidant activity. Analyst 2002, 127, 183–198. [Google Scholar] [CrossRef]
- Thaipong, K.; Boonprakob, U.; Crosby, K.; Cisneros-Zevallos, L.; Byrne, D.H. Comparison of ABTS, DPPH, FRAP, and ORAC assays for estimating antioxidant activity from guava fruit extracts. J. Food Compos. Anal. 2006, 19, 669–675. [Google Scholar] [CrossRef]
- Arena, E.; Fallico, B.; Maccarone, E. Evaluation of antioxidant capacity of blood orange juices as influenced by constituents, concentration process and storage. Food Chem. 2001, 74, 423–427. [Google Scholar] [CrossRef]
- Satué-Gracia, M.T.; Heinonen, M.; Ankel, E.N. Anthocyanin as antioxidants on human low-density lipoprotein and lecithin-liposome systems. J. Agric. Food Chem. 1997, 45, 3362–3367. [Google Scholar] [CrossRef]
- Kuskoski, E.M.; Asuero, A.G.; Troncoso, A.M.; Mancini-Filho, J.; Fett, R. Aplicação de diversos métodos químicos para determinar atividade antioxidante em polpa de frutas. Ciênc. Tecnol. Aliment.Camp. 2005, 25, 726–732. [Google Scholar] [CrossRef]
- Chavez-Santoscoy, R.A.; Gutierrez-Uribe, J.A.; Serna-Saldívar, S.O. Phenolic composition, antioxidant capacity and in vitro cancer cell cytotoxicity of nine prickly pear (Opuntia spp.) juices. Plant Foods Hum. Nutr. 2009, 64, 146–152. [Google Scholar] [CrossRef]
- Guruprasad, K.P.; Subramanian, A.; Singh, V.J.; Sharma, R.S.; Gopinath, P.M.; Sewram, V.; Varier, P.M.; Satyamoorthy, K. Brahmarasayana protects against ethyl methanesulfonate or methyl methanesulfonate induced chromosomal aberrations in mouse bone marrow cells. BMC Complement. Altern. Med. 2012, 12, 113. [Google Scholar] [CrossRef]
- Kim, D.-O.; Lee, K.W.; Lee, H.J.; Lee, C.Y. Vitamin C equivalent antioxidant capacity (VCEAC) of phenolics phytochemicals. J. Agric. Food Chem. 2002, 50, 3713–3717. [Google Scholar]
- Madrigal-Santillán, E.; Fragoso-Antonio, S.; Valadez-Vega, C.; Solano-Solano, G.; Zúñiga Pérez, C.; Sánchez-Gutiérrez, M.; Izquierdo-Vega, J.A.; Gutiérrez-Salinas, J.; Esquivel-Soto, J.; Esquivel-Chirino, C.; et al. Investigation on the protective effect of cranberry against the DNA damage induced by benzo(a)pyrene. Molecules 2012, 17, 4435–4451. [Google Scholar]
- Butera, D.; Tesoriere, L.; Di Gaudio, F.; Bongiorno, A.; Allegra, M.; Pintaudi, A.M.; Kohen, R.; Livrea, M.A. Antioxidantactivities of sicilianpricklypear (Opuntia ficus indica) fruitextracts and reducingproperties of its betalains: Betanin and indicaxanthin. J. Agric. Food Chem. 2002, 6, 6895–6901. [Google Scholar]
- Tesoriere, L.; Butera, D.; Allegra, M.; Fazzari, M.; Livrea, M.A. Distribution of betalainpigments in redbloodcellsafter consumption of cactus pear fruits and increased resistance of the cells to ex vivo induced oxidative hemolysis in humans. J. Agric. Food Chem. 2005, 53, 1266–1270. [Google Scholar] [CrossRef]
- Yahia, E.M.; Mondragon-Jacobo, C. Nutritional components and anti-oxidant capacity of ten cultivars and lines of cactus pear fruit (Opuntia spp.). Food Res. Int. 2011, 44, 2311–2318. [Google Scholar] [CrossRef]
- Hernández-Ceruelos, A.; Madrigal-Santillán, E.; Morales-González, J.A.; Chamorro-Cevallos, G.; Cassani-Galindo, M.; Madrigal-Bujaidar, E. Antigenotoxic effect of chamomilla recutita (L.) rauschert essential oil in mouse spermatogonial cells, and determination of its antioxidant capacity in vitro. Int. J. Mol. Sci. 2010, 11, 3793–3802. [Google Scholar] [CrossRef]
- Fernández-Sánchez, A.; Madrigal-Santillán, E.; Bautista, M.; Esquivel-Soto, J.; Morales-González, A.; Esquivel-Chirino, C.; Durante-Montiel, I.; Sánchez-Rivera, G.; Valadez-Vega, C.; Morales-González, J.A. Inflammation, oxidative stress, and obesity. Int. J. Mol. Sci. 2011, 12, 3117–3132. [Google Scholar] [CrossRef]
- Haveland-Smith, R.B. Evaluation of the genotoxicity of some natural food colours using bacterial assays. Mutat. Res. 1981, 91, 285–289. [Google Scholar] [CrossRef]
- Schwartz, S.J.; von Elbe, J.H.; Pariza, M.W.; Goldsworthy, T.; Pitot, H.C. Inability of red beet betalain pigments to initiate or promote hepatocarcinogenesis. Food Chem. Toxicol. 1983, 21, 531–535. [Google Scholar] [CrossRef]
- Franke, S.I.; Prá, D.; Erdtmann, B.; Henriques, J.A.; da Silva, J. Influence of orange juice over the genotoxicity induced by alkylating agents: An in vivo analysis. Mutagenesis 2005, 20, 279–283. [Google Scholar] [CrossRef]
- Virgili, F.; Marino, M. Regulation of cellular signals from nutritional molecules: A specific role for phytochemicals, beyond antioxidant activity. Free Radic. Biol. Med. 2008, 45, 1205–1216. [Google Scholar] [CrossRef]
- Brahmi, D.; Bouaziz, C.; Ayed, Y.; Ben Mansour, H.; Zourgui, L.; Bacha, H. Chemopreventiveeffect of cactus Opuntia ficus indica on oxidative stress and genotoxicity of aflatoxin B1. Nutr. Metab. 2011, 8, 73. [Google Scholar] [CrossRef]
- Brahmi, D.; Ayed, Y.; Hfaiedh, M.; Bouaziz, C.; Mansour, H.B.; Zourgui, L.; Bacha, H. Protective effect of cactus cladode extract against cisplatin induced oxidative stress, genotoxicity and apoptosis in balb/c mice: Combination with phytochemical composition. BMC Complement. Altern. Med. 2012, 12, 111. [Google Scholar] [CrossRef]
- Brahmi, D.; Ayed, Y.; Bouaziz, C.; Zourgui, L.; Hassen, W.; Bacha, H. Hepatoprotective effect of cactus extract against carcinogenicity of benzo(a)pyrene on liver of Balb/C Mice. J. Med. Plants Res. 2011, 5, 4627–4639. [Google Scholar]
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Madrigal-Santillán, E.; García-Melo, F.; Morales-González, J.A.; Vázquez-Alvarado, P.; Muñoz-Juárez, S.; Zuñiga-Pérez, C.; Sumaya-Martínez, M.T.; Madrigal-Bujaidar, E.; Hernández-Ceruelos, A. Antioxidant and Anticlastogenic Capacity of Prickly Pear Juice. Nutrients 2013, 5, 4145-4158. https://doi.org/10.3390/nu5104145
Madrigal-Santillán E, García-Melo F, Morales-González JA, Vázquez-Alvarado P, Muñoz-Juárez S, Zuñiga-Pérez C, Sumaya-Martínez MT, Madrigal-Bujaidar E, Hernández-Ceruelos A. Antioxidant and Anticlastogenic Capacity of Prickly Pear Juice. Nutrients. 2013; 5(10):4145-4158. https://doi.org/10.3390/nu5104145
Chicago/Turabian StyleMadrigal-Santillán, Eduardo, Fernando García-Melo, José A. Morales-González, Patricia Vázquez-Alvarado, Sergio Muñoz-Juárez, Clara Zuñiga-Pérez, Maria Teresa Sumaya-Martínez, Eduardo Madrigal-Bujaidar, and Alejandra Hernández-Ceruelos. 2013. "Antioxidant and Anticlastogenic Capacity of Prickly Pear Juice" Nutrients 5, no. 10: 4145-4158. https://doi.org/10.3390/nu5104145