Chemopreventive Effects of Oplopantriol A, a Novel Compound Isolated from Oplopanax horridus, on Colorectal Cancer
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals and Reagents
2.2. Plant Material, Isolation and Structural Elucidation
2.3. Human Cancer Cell Lines and Cell Culture
2.4. Xenograft Tumor Xenogen Bioluminescence Imaging
2.5. MTS Assays
2.6. Apoptosis Assay
2.7. Cell Cycle Assay
2.8. Real-Time PCR Array of Apoptosis Screening Analysis
2.9. Statistical Analysis
3. Results
3.1. Oplopantriol A Inhibited Colon Tumor Growth
3.2. Oplopantriol A Inhibited HCT-116 and SW-480 Cell Proliferation
3.3. Oplopantriol A Induced Apoptosis
3.4. Oplopantriol A Arrested Cell Cycle at the G2/M Phase
3.5. Oplopantriol A Induced Apoptosis Related Gene Expression and TNF-Cell Death Pathways
4. Discussion
5. Conclusions
Acknowledgments
Conflicts of Interest
References
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Zhang, Z.; Yu, C.; Zhang, C.-F.; Wu, X.-H.; Wen, X.-D.; Anderson, S.; Du, W.; Huang, W.-H.; Li, S.-P.; Wang, C.-Z.; et al. Chemopreventive Effects of Oplopantriol A, a Novel Compound Isolated from Oplopanax horridus, on Colorectal Cancer. Nutrients 2014, 6, 2668-2680. https://doi.org/10.3390/nu6072668
Zhang Z, Yu C, Zhang C-F, Wu X-H, Wen X-D, Anderson S, Du W, Huang W-H, Li S-P, Wang C-Z, et al. Chemopreventive Effects of Oplopantriol A, a Novel Compound Isolated from Oplopanax horridus, on Colorectal Cancer. Nutrients. 2014; 6(7):2668-2680. https://doi.org/10.3390/nu6072668
Chicago/Turabian StyleZhang, Zhiyu, Chunhao Yu, Chun-Feng Zhang, Xiao-Hui Wu, Xiao-Dong Wen, Samantha Anderson, Wei Du, Wei-Hua Huang, Shao-Ping Li, Chong-Zhi Wang, and et al. 2014. "Chemopreventive Effects of Oplopantriol A, a Novel Compound Isolated from Oplopanax horridus, on Colorectal Cancer" Nutrients 6, no. 7: 2668-2680. https://doi.org/10.3390/nu6072668