Leucine Promotes Proliferation and Differentiation of Primary Preterm Rat Satellite Cells in Part through mTORC1 Signaling Pathway
Abstract
:1. Introduction
2. Experimental Section
2.1. Animals
2.2. Reagents
2.3. Primary Culture of Preterm Rat Satellite Cells
2.4. Differentiation Culture
2.5. Immunocytochemical
2.6. Cell Proliferation Rate Assay
2.7. Western Blot
2.8. Statistical Analysis
3. Results
3.1. Culture and Identification of Primary Preterm Rat Satellite Cells
3.2. Leucine Promotes Primary Satellite Cells Proliferation Through mTORC1 Pathway
3.3. Leucine Promotes Differentiation of Primary Preterm Rat Satellite Cells
3.4. Involvement of mTORC1 in Leucine-Stimulated Differentiation of Primary Satellite Cells
4. Discussion
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Dai, J.-M.; Yu, M.-X.; Shen, Z.-Y.; Guo, C.-Y.; Zhuang, S.-Q.; Qiu, X.-S. Leucine Promotes Proliferation and Differentiation of Primary Preterm Rat Satellite Cells in Part through mTORC1 Signaling Pathway. Nutrients 2015, 7, 3387-3400. https://doi.org/10.3390/nu7053387
Dai J-M, Yu M-X, Shen Z-Y, Guo C-Y, Zhuang S-Q, Qiu X-S. Leucine Promotes Proliferation and Differentiation of Primary Preterm Rat Satellite Cells in Part through mTORC1 Signaling Pathway. Nutrients. 2015; 7(5):3387-3400. https://doi.org/10.3390/nu7053387
Chicago/Turabian StyleDai, Jie-Min, Mu-Xue Yu, Zhen-Yu Shen, Chu-Yi Guo, Si-Qi Zhuang, and Xiao-Shan Qiu. 2015. "Leucine Promotes Proliferation and Differentiation of Primary Preterm Rat Satellite Cells in Part through mTORC1 Signaling Pathway" Nutrients 7, no. 5: 3387-3400. https://doi.org/10.3390/nu7053387