Aging Reduces the Activation of the mTORC1 Pathway after Resistance Exercise and Protein Intake in Human Skeletal Muscle: Potential Role of REDD1 and Impaired Anabolic Sensitivity
Abstract
:1. Introduction
2. Materials and Methods
2.1. Subjects
Young | Older | |
---|---|---|
Number (n) | 9 M | 6 M + 4 F |
Age (years) | 22 ± 0.1 | 69 ± 1.7 *** |
Weight (kg) | 78 ± 2.0 | 79 ± 3.5 |
Height (cm) | 177 ± 2.5 | 174 ± 3.2 |
BMI (kg/m2) | 25 ± 1.0 | 26 ± 0.9 |
Total load lifted (kg) | 2271 ± 51.4 | M + W: 1194 ± 86.3 *** M only: 1365 ± 84.3 *** W only: 981 ± 75.0 ***, # |
2.2. Protocol
2.3. Blood Chemistry
2.4. Western Blotting
2.5. Real-Time Quantitative Polymerase Chain Reaction
Forward | Reverse | |
---|---|---|
BNIP3 | CTG AAA CAG ATA CCC ATA GCA TT | CCG ACT TGA CCA ATC CCA |
BNIP3L | CCA AGG AGT TCC ACT TCA GAC | AGT AGG TGC TGG CAG AGG GTG T |
CycloA | CTT CAT CCT AAA GCA TAC GGG TC | TGC CAT CCA ACC ACT CAG TCT |
GABARAP | GTG CCC TCT GAC CTT ACT GTT G | CAT TTC CCA TAG ACA CTC TCA TC |
IGF-I | TAT TTC AAC AAG CCC ACA GG | CAT CTC CAG CCT CCT TAG AT |
IGF-II | TGG ACA CCC TCC AGT TC | GGA AAC AGC ACT CCT CAAC |
HIF-1α | GCC CCA GAT TCA GGA TCA GA | TGG GAC TAT TAG GCT CAG GTG AAC |
LC3b | AAT CCC GGT GAT AAT AGA ACG A | GGA GAC GCT GAC CAT GCT GT |
MafBx | CGA CCT CAG CAG TTA CTG CAA C | TTT GCT ATC AGC TCC AAC AG |
MuRF-1 | AAA CAG GAG TGC TCC AGT CGG | CGC CAC CAG CAT GGA GAT ACA |
p62 | CCT CTG GGC ATT GAA GTT G | TAT CCG ACT CCA TCT GTT CCTC |
RPL4 | ATA CGC CAT CTG TTC TGC CCT | GCT TCC TTG GTC TTC TTG TAG CCT |
2.6. Statistical Analysis
3. Results
3.1. Blood Parameters
Young | Older | |||
---|---|---|---|---|
Fasted | Pro+ex | Fasted | Pro+ex | |
Glucose (mg/dL) | 90.2 ± 1.33 | 100.3 ± 2.21 §§§ | 97.5 ± 2.65 * | 111.8 ± 2.43 §§§,*** |
Insulin (µU/mL) | 10.3 ± 2.93 | 26.2 ± 4.72 §§ | 9.5 ± 1.17 | 39.7 ± 6.51 §§,* |
Isoleucine (µmol/L) | 87 ± 7.1 | 164 ± 9.5 §§§ | 78 ± 5.6 | 141 ± 9.0 §§§,* |
Leucine (µmol/L) | 171 ± 7.6 | 408 ± 28.2 §§§ | 155 ± 10.2 | 353 ± 24.7 §§§ |
Valine (µmol/L) | 285 ± 14.0 | 358 ± 18.8 §§§ | 280 ± 15.2 | 336 ± 15.9 §§§ |
3.2. PKB Response to Exercise and Feeding is Blunted in Old Subjects
3.3. Markers for Autophagy and the Ubiquitin-Proteasome System Are Not Altered with Age
3.4. Basal Insulin-Like Growth Factor-1 and Hypoxia-Inducible Factor 1 Alpha MRNA LEVELS Are Reduced in Old Subjects
4. Discussion
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Francaux, M.; Demeulder, B.; Naslain, D.; Fortin, R.; Lutz, O.; Caty, G.; Deldicque, L. Aging Reduces the Activation of the mTORC1 Pathway after Resistance Exercise and Protein Intake in Human Skeletal Muscle: Potential Role of REDD1 and Impaired Anabolic Sensitivity. Nutrients 2016, 8, 47. https://doi.org/10.3390/nu8010047
Francaux M, Demeulder B, Naslain D, Fortin R, Lutz O, Caty G, Deldicque L. Aging Reduces the Activation of the mTORC1 Pathway after Resistance Exercise and Protein Intake in Human Skeletal Muscle: Potential Role of REDD1 and Impaired Anabolic Sensitivity. Nutrients. 2016; 8(1):47. https://doi.org/10.3390/nu8010047
Chicago/Turabian StyleFrancaux, Marc, Bénédicte Demeulder, Damien Naslain, Raphael Fortin, Olivier Lutz, Gilles Caty, and Louise Deldicque. 2016. "Aging Reduces the Activation of the mTORC1 Pathway after Resistance Exercise and Protein Intake in Human Skeletal Muscle: Potential Role of REDD1 and Impaired Anabolic Sensitivity" Nutrients 8, no. 1: 47. https://doi.org/10.3390/nu8010047
APA StyleFrancaux, M., Demeulder, B., Naslain, D., Fortin, R., Lutz, O., Caty, G., & Deldicque, L. (2016). Aging Reduces the Activation of the mTORC1 Pathway after Resistance Exercise and Protein Intake in Human Skeletal Muscle: Potential Role of REDD1 and Impaired Anabolic Sensitivity. Nutrients, 8(1), 47. https://doi.org/10.3390/nu8010047