Walnut Polyphenol Extract Attenuates Immunotoxicity Induced by 4-Pentylphenol and 3-methyl-4-nitrophenol in Murine Splenic Lymphocyte
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Experimental Animals
2.3. Preparation of WPE
2.4. Preparation of Splenocytes
2.5. Cell Viability Assay
2.6. Cell Staining/Flow Cytometry for Phenotypic Analysis
2.7. Measurement of Cytokine/Granzyme Production and Determination of ·OH, SOD, GSH-Px, and MDA Levels
2.8. Determination of the Total Phenolic Content
2.9. LC-MS Analyses (HPLC-ESI-IT-TOF-MS)
2.10. Statistical Analysis
3. Results
3.1. WPE Attenuates Cytotoxicity in Splenocytes
3.2. WPE Inhibited Decreases in Splenic T Cell Sub-Populations
3.3. Treatment with WPE Prevents the Loss of Splenic T Cell Activity
3.4. WPE Attenuates PP- and PNMC-Induced Oxidative Damage in Splenocytes
3.5. Quantification and Characterization of Phenolic Compounds in WPE
4. Discussion
5. Conclusions
Acknowledgments
Author Contribution
Conflicts of Interest
References
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No. | tR (min) | Measured [M − H]−(m/z) | Predicted [M − H]− (m/z) | λ (nm) | Molecular Formula | Identification | Expressed as | Reference |
---|---|---|---|---|---|---|---|---|
1 | 12.663, 12.906 | 300.9993 | 300.9990 | 280 | C14H6O8 | Ellagic aci | Ellagitannins | [37,38,39] |
2 | 11.946 | 433.0432 | 433.0412 | 280 | C19H14O12 | Ellagic acid 4-O-xyloside | Ellagitannins | [37,38,39] |
3 | 12.507 | 433.1119 | 433.0772 | 280 | C20H18O11 | Quercetin pentoside isomer | Quercetin | [38] |
4 | 11.946 | 456.0383 | 457.0781 | 280 | C22H17O11 | Epigallocatechin-3-O-gallate | Ellagitannins | [40] |
5 | 13.74 | 447.0831 | 447.0938 | 280 | C21H19O11 | Kaempferol-3-O-glucoside | Ellagitannins | [40] |
6 | 12.507, 12.663, 12.906 | 469.0492 | 469.0049 | 280 | C21H10O13 | Valoneic acid dilactone | Valoneic acid dilactone | [38] |
7 | 11.946 | 481.5355 | 481.0620 | 280 | C20H18O14 | 2,3-O-HHDP-d-glucoside | Ellagitannins | [38] |
8 | 9.828, 10.020, 10.512, 10.784 | 483.5192 | 483.0777 | 280 | C20H20O14 | Digalloyl-glucose isomer | Gallic acid | [38,39] |
9 | 12.25 | 615.5905 | 615.0986 | 280 | C28H24O16 | Quercetin galloylhexoside isomer | Quercetin | [38] |
10 | 8.382, 8.751, 9.624 | 633.0720 | 633.0720 | 280 | C27H22O18 | Strictinin (galloyl-HHDP-glucose) | Ellagitannins | [37,38,39] |
11 | 10.784, 11.136 | 635.0876 | 635.0877 | 280 | C27H24O18 | Trigalloyl-glucose isomer | Ellagitannins | [38] |
12 | 8.382, 8.751, 9.003 | 783.0685 | 783.0681 | 280 | C34H24O22 | Pedunculagin (bis-HHDP-glucose) | Ellagitannins | [37,38,39] |
13 | 10.020, 10.236 | 785.0808 | 785.0840 | 280 | C34H26O22 | Tellimagrandin I isomer(digalloyl-HHDP-glucose) | Ellagitannins | [37,38,39] |
14 | 11.946, 12.250 | 787.1144 | 787.0996 | 280 | C34H28O22 | Tetragalloyl-glucose | Ellagitannins | [38] |
15 | 10.784, 11.488 | 933.0316 | 933.0630 | 280 | C41H26O26 | Praecoxin D | Ellagitannins | [37,38,39] |
16 | 11.136 | 935.0746 | 935.0786 | 280 | C41H28O26 | Casuarinin(Galloyl bis HHDP glucose) | Ellagitannins | [37,38,39] |
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Yang, L.; Ma, S.; Han, Y.; Wang, Y.; Guo, Y.; Weng, Q.; Xu, M. Walnut Polyphenol Extract Attenuates Immunotoxicity Induced by 4-Pentylphenol and 3-methyl-4-nitrophenol in Murine Splenic Lymphocyte. Nutrients 2016, 8, 287. https://doi.org/10.3390/nu8050287
Yang L, Ma S, Han Y, Wang Y, Guo Y, Weng Q, Xu M. Walnut Polyphenol Extract Attenuates Immunotoxicity Induced by 4-Pentylphenol and 3-methyl-4-nitrophenol in Murine Splenic Lymphocyte. Nutrients. 2016; 8(5):287. https://doi.org/10.3390/nu8050287
Chicago/Turabian StyleYang, Lubing, Sihui Ma, Yu Han, Yuhan Wang, Yan Guo, Qiang Weng, and Meiyu Xu. 2016. "Walnut Polyphenol Extract Attenuates Immunotoxicity Induced by 4-Pentylphenol and 3-methyl-4-nitrophenol in Murine Splenic Lymphocyte" Nutrients 8, no. 5: 287. https://doi.org/10.3390/nu8050287