Melatonin Modulates Neuronal Cell Death Induced by Endoplasmic Reticulum Stress under Insulin Resistance Condition
Abstract
:1. Introduction
2. Methods and Materials
2.1. Cell Culture
2.2. Cell Viability Assay
2.3. Reverse Transcription-PCR
2.4. Quantitative Real Time-PCR
2.5. Western Blot Analysis
2.6. Immunofluorescence for p-ASK-1 and p-IRE1
2.7. Statistical Analysis
3. Results
3.1. Melatonin Increases SH-SY5Y Cell Viability under IR Conditions
3.2. Melatonin Treatment Alleviates IR-Induced Neuronal Cell Death Signaling
3.3. Melatonin Treatment Regulates IR-Induced ER Stress Signaling in Neuronal Cells
3.4. Melatonin Attenuates the Activation of ASK1 under IR Condition
4. Discussion
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Song, J.; Kim, O.Y. Melatonin Modulates Neuronal Cell Death Induced by Endoplasmic Reticulum Stress under Insulin Resistance Condition. Nutrients 2017, 9, 593. https://doi.org/10.3390/nu9060593
Song J, Kim OY. Melatonin Modulates Neuronal Cell Death Induced by Endoplasmic Reticulum Stress under Insulin Resistance Condition. Nutrients. 2017; 9(6):593. https://doi.org/10.3390/nu9060593
Chicago/Turabian StyleSong, Juhyun, and Oh Yoen Kim. 2017. "Melatonin Modulates Neuronal Cell Death Induced by Endoplasmic Reticulum Stress under Insulin Resistance Condition" Nutrients 9, no. 6: 593. https://doi.org/10.3390/nu9060593