Detection of Aflatoxigenic and Atoxigenic Mexican Aspergillus Strains by the Dichlorvos–Ammonia (DV–AM) Method
Abstract
:1. Introduction
2. Results
2.1. Isolation of Aspergilli from Soil Subsamples
2.2. Determination of AFs
2.3. Phylogenetic Analysis of Isolates
3. Discussion
4. Conclusions
5. Materials and Methods
5.1. Media
5.2. Strains Used
5.3. Soil Sampling
5.4. DV–AM Method
5.5. AF Production Analysis
5.6. Identification of Fungal Species
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
DV-AM | dichlorvos-ammonia |
AF | aflatoxin |
DV | dichlorvos |
VOAc | versiconol acetate |
VHA | versiconal hemiacetal acetate |
AFPA | Aspergillus flavus and parasiticus agar |
TFA | trifluoroacetic acid |
HPLC | High-performance liquid chromatography |
DNA | deoxyribonucleic acid |
PCR | polymerase chain reaction |
DDBJ | DNA Data Bank of Japan |
EMBL | European Molecular Biology Laboratory |
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Kushiro, M.; Hatabayashi, H.; Yabe, K.; Loladze, A. Detection of Aflatoxigenic and Atoxigenic Mexican Aspergillus Strains by the Dichlorvos–Ammonia (DV–AM) Method. Toxins 2018, 10, 263. https://doi.org/10.3390/toxins10070263
Kushiro M, Hatabayashi H, Yabe K, Loladze A. Detection of Aflatoxigenic and Atoxigenic Mexican Aspergillus Strains by the Dichlorvos–Ammonia (DV–AM) Method. Toxins. 2018; 10(7):263. https://doi.org/10.3390/toxins10070263
Chicago/Turabian StyleKushiro, Masayo, Hidemi Hatabayashi, Kimiko Yabe, and Alexander Loladze. 2018. "Detection of Aflatoxigenic and Atoxigenic Mexican Aspergillus Strains by the Dichlorvos–Ammonia (DV–AM) Method" Toxins 10, no. 7: 263. https://doi.org/10.3390/toxins10070263