The Activity of Isoquinoline Alkaloids and Extracts from Chelidonium majus against Pathogenic Bacteria and Candida sp.
Abstract
:1. Introduction
2. Results
2.1. Comparison of Extraction Effectiveness
2.2. MIC Evaluation
2.3. Cytotoxicity
3. Discussion
4. Conclusions
5. Materials and Methods
5.1. Plant Material
5.1.1. Wild Growing Plants
5.1.2. In Vitro Plant Material
5.2. Phytochemistry
5.2.1. Reagents and Standards
5.2.2. Sample Preparation
5.2.3. HPLC Analysis
5.3. Statistical Analysis
5.4. Experimental Design for Bioactivity Assays
5.4.1. Strains
5.4.2. MIC Evaluation
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Solvent/Plant Material | Candida albicans | Staphylococcus aureus |
---|---|---|
MeOH aerial | − | − |
MeOH roots | − | 62.5 * |
Culture medium/plant material | Candida albicans | Staphylococcus aureus |
MS roots | 125 | 500 * |
MS shoots | 500 * | 500 * |
MS+N roots | 3.9 | 500 * |
MS+N shoots | 3.9 | 500 * |
½ MS roots | − | − |
½ MS shoots | − | − |
1.5%suc. + N roots | − | − |
1.5%suc.+ N shoots | 500 | 250 |
1.5%suc. roots | 250 | 125 |
1.5%suc. shoots | 500 | 500 * |
B5 roots | − | 250 * |
B5 shoots | − | 500 * |
B5 + N roots | − | − |
B5 + N shoots | − | 250 * |
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Zielińska, S.; Wójciak-Kosior, M.; Dziągwa-Becker, M.; Gleńsk, M.; Sowa, I.; Fijałkowski, K.; Rurańska-Smutnicka, D.; Matkowski, A.; Junka, A. The Activity of Isoquinoline Alkaloids and Extracts from Chelidonium majus against Pathogenic Bacteria and Candida sp. Toxins 2019, 11, 406. https://doi.org/10.3390/toxins11070406
Zielińska S, Wójciak-Kosior M, Dziągwa-Becker M, Gleńsk M, Sowa I, Fijałkowski K, Rurańska-Smutnicka D, Matkowski A, Junka A. The Activity of Isoquinoline Alkaloids and Extracts from Chelidonium majus against Pathogenic Bacteria and Candida sp. Toxins. 2019; 11(7):406. https://doi.org/10.3390/toxins11070406
Chicago/Turabian StyleZielińska, Sylwia, Magdalena Wójciak-Kosior, Magdalena Dziągwa-Becker, Michał Gleńsk, Ireneusz Sowa, Karol Fijałkowski, Danuta Rurańska-Smutnicka, Adam Matkowski, and Adam Junka. 2019. "The Activity of Isoquinoline Alkaloids and Extracts from Chelidonium majus against Pathogenic Bacteria and Candida sp." Toxins 11, no. 7: 406. https://doi.org/10.3390/toxins11070406