Reply to Comments on Proteomic Investigations of Two Pakistani Naja Snake Venom Species Unravel the Venom Complexity, Posttranslational Modifications, and Presence of Extracellular Vesicles. Toxins 2020, 12, 669
- Every separation step underlies the law of mass action, thereby multiplying the error with the number of fractionation steps. Because of this problem, in analytical chemistry internal standards are used;
- A single Coomassie stained band does contain much more than one protein. With modern methods of proteomics, we usually detect dozens, very often hundreds of proteins in one single SDS-PAGE band. Even in spots of two-dimensional electrophoresis, we identify on average more than 100 proteins.
- (1)
- The absolute pharmacological action of a single venom depends not only on the absolute quantity, but also on its potency (with respect to its enzymatic activity);
- (2)
- The potency of a protein in the venom depends on its exact composition of atoms, and of course the three-dimensional fold. Already, small changes in the composition of atoms and the sequence and location of amino acids can drastically change the action and alter the potency of the function of a protein (please refer to the respective publications about protein species, proteoforms (synonyms), e.g., references [2,3,4,5].
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References
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Munawar, A.; Dreyer, B.; Schlüter, H.; Betzel, C. Reply to Comments on Proteomic Investigations of Two Pakistani Naja Snake Venom Species Unravel the Venom Complexity, Posttranslational Modifications, and Presence of Extracellular Vesicles. Toxins 2020, 12, 669. Toxins 2020, 12, 781. https://doi.org/10.3390/toxins12120781
Munawar A, Dreyer B, Schlüter H, Betzel C. Reply to Comments on Proteomic Investigations of Two Pakistani Naja Snake Venom Species Unravel the Venom Complexity, Posttranslational Modifications, and Presence of Extracellular Vesicles. Toxins 2020, 12, 669. Toxins. 2020; 12(12):781. https://doi.org/10.3390/toxins12120781
Chicago/Turabian StyleMunawar, Aisha, Benjamin Dreyer, Hartmut Schlüter, and Christian Betzel. 2020. "Reply to Comments on Proteomic Investigations of Two Pakistani Naja Snake Venom Species Unravel the Venom Complexity, Posttranslational Modifications, and Presence of Extracellular Vesicles. Toxins 2020, 12, 669" Toxins 12, no. 12: 781. https://doi.org/10.3390/toxins12120781