Charged Residues Flanking the Transmembrane Domain of Two Related Toxin–Antitoxin System Toxins Affect Host Response
Abstract
:1. Introduction
2. Results
2.1. Importance of the Charged C-Terminal Tail to Fst Toxicity
2.2. Identification of the Key Residues for the Differential Response of RS02610
2.3. The Differential Response of RS01655 May Relate to Its Function as an Efflux Pump
3. Discussion
4. Materials and Methods
4.1. Bacterial Strains, Media, and Growth Conditions
4.2. Genetic Manipulations
4.3. RNA Purification, Manipulation, and Statistical Methodology
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Mutant Fst toxin | Sequence a |
---|---|
FstpAD1 | VKDLMSLVIAPIFVGLVLEMISRVLDEEDDSRK |
pAD1ter2 | VKDLMSLVIAPIFVGLVLEMISRVLDEEDDS |
pAD1ter5 | VKDLMSLVIAPIFVGLVLEMISRVLDEE |
pAD1ter7 | VKDLMSLVIAPIFVGLVLEMISRVLD |
FstEF0409 | MYEIVTKILVPIFVGIVLKLVTIWLEKQNEE |
EF0409ter2 | MYEIVTKILVPIFVGIVLKLVTIWLEKQN |
EF0409ter5 | MYEIVTKILVPIFVGIVLKLVTIWLE |
pAD1EF0409:6 | VKDLMSLVIAPIFVGLVLEMISRVLEKQNEE |
EF0409pAD1:8 | MYEIVTKILVPIFVGIVLKLVTIWLDEEDDSRK |
EF0409pAD1:10 | MYEIVTKILVPIFVGIVLKLVTIVLDEEDDSRK |
EF0409pAD1:12 | MYEIVTKILVPIFVGIVLKLVSRVLDEEDDSRK |
EF0409pAD1:14 | MYEIVTKILVPIFVGIVLKMISRVLDEEDDSRK |
EF0409pAD1:15 | MYEIVTKILVPIFVGIVLEMISRVLDEEDDSRK |
EF0409 K19E | MYEIVTKILVPIFVGIVLELVTIWLEKQNEE |
pAD1 E19K | VKDLMSLVIAPIFVGLVLKMISRVLDEEDDSRK |
EF0409pAD1N6-K19E | MKDLMSLILVPIFVGIVLELVTIWLEKQNEE |
EF0409 K7L-K19E | MYEIVTLILVPIFVGIVLELVTIWLEKQNEE |
pAD1 L7K-E19K | VKDLMSKVIAPIFVGLVLKMISRVLDEEDDSRK |
EF0409pAD1FL | MYEIVTKILVPIFVGIVLKLVFLVLDEEDDSRK |
Primer | Sequence |
---|---|
pCIE-EF0409 FWD | GTATACAGTTCATGTATATGTTCCC |
pCIE-EF0409 REV | TGTGATGCACCTCCTTTC |
RS02610 FWD | CAGATGACGGCTCAATTCAAAC |
RS02610 REV | CAGCGGTACTTCCTTCAATCA |
RS01655 FWD | GCACGATGTCTGGTGATGAT |
RS01655 REV | CTTCGCTCCTAAATCCGCTAAG |
mgtA FWD | AAAGGTGCGGTTGAAGAAATG |
mgtA REV | TGACGCAGTGTCTCTGTTAAG |
celA3 FWD | AGAAGATCGTGGCATGGAAG |
celA3 REV | TGAAACGAACTTGTGGACCTAA |
GyrB FWD | ACCAACACCGTGCAAGCC |
GyrB REV | CAAGCCAAAACAGGTCGCC |
Delta01655 FWD | GGGTCCTTCTGTGTGTGTAAATA |
Delta01655 REV | GTCCACTCGGCTAAACGTATAAT |
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Holmes, A.; Sadlon, J.; Weaver, K. Charged Residues Flanking the Transmembrane Domain of Two Related Toxin–Antitoxin System Toxins Affect Host Response. Toxins 2021, 13, 329. https://doi.org/10.3390/toxins13050329
Holmes A, Sadlon J, Weaver K. Charged Residues Flanking the Transmembrane Domain of Two Related Toxin–Antitoxin System Toxins Affect Host Response. Toxins. 2021; 13(5):329. https://doi.org/10.3390/toxins13050329
Chicago/Turabian StyleHolmes, Andrew, Jessie Sadlon, and Keith Weaver. 2021. "Charged Residues Flanking the Transmembrane Domain of Two Related Toxin–Antitoxin System Toxins Affect Host Response" Toxins 13, no. 5: 329. https://doi.org/10.3390/toxins13050329