Simplified Synthesis and Stability Assessment of Aflatoxin B1-Lysine and Aflatoxin G1-Lysine
Abstract
:1. Introduction
2. Results
2.1. Conversion of AFB1 to AFB2a and AFG1 to AFG2a
2.2. Coupling of AFB2a or AFG2a to Lysine
2.3. Oxidation of AFB2a-Lys to AFB1-Lys
2.4. Synthesis of Isotopically Labelled Standards
2.5. Quantitative NMR
2.6. Stability
3. Conclusions
4. Materials and Methods
4.1. Materials
4.2. Conversion of AFB1/AFG1 to AFB2a/AFG2a
4.3. Coupling of Lysine to AFB2a
4.4. Oxidation of AFB2a-Lysine to AFB1-Lysine
4.5. Purification by HPLC
4.6. Quantitative NMR
4.7. Stability Assay
Supplementary Materials
Author Contributions
Funding
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Compound | ε (pH 7.4) | Concentration by UV–VIS (mM) | Concentration by qNMR (mM) |
---|---|---|---|
AFB1-Lys | 30,866 | 1.15 | 1.01 |
AFG1-Lys | 27,783 | 0.634 | 1.89 |
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Renaud, J.B.; Walsh, J.P.; Sumarah, M.W. Simplified Synthesis and Stability Assessment of Aflatoxin B1-Lysine and Aflatoxin G1-Lysine. Toxins 2022, 14, 56. https://doi.org/10.3390/toxins14010056
Renaud JB, Walsh JP, Sumarah MW. Simplified Synthesis and Stability Assessment of Aflatoxin B1-Lysine and Aflatoxin G1-Lysine. Toxins. 2022; 14(1):56. https://doi.org/10.3390/toxins14010056
Chicago/Turabian StyleRenaud, Justin B., Jacob P. Walsh, and Mark W. Sumarah. 2022. "Simplified Synthesis and Stability Assessment of Aflatoxin B1-Lysine and Aflatoxin G1-Lysine" Toxins 14, no. 1: 56. https://doi.org/10.3390/toxins14010056