A Sea Anemone Lebrunia neglecta Venom Fraction Decreases Boar Sperm Cells Capacitation: Possible Involvement of HVA Calcium Channels
Abstract
:1. Introduction
2. Results and Discussion
2.1. Venom Extraction and Fractionation
2.2. Sperm Viability
2.3. Sperm Motility
2.4. Capacitation
2.5. Effect of F3 on Calcium Current Amplitude
2.6. F3 Inhibits Calcium Currents without Changes in Voltage Dependence
2.7. F3 Does Not Inhibit L-Type Calcium Channels Only
3. Materials and Methods
3.1. Venom Extraction and Fractionation
3.2. Protein Quantification
3.3. Mass Spectrometry Analysis
3.4. Collection and Semen Sample Preparation
3.5. Capacitation
3.6. Toxin Exposure
3.7. Chromaffin Cells Culture
3.8. Electrophysiological Recording
3.9. Statistical Analysis
4. Ethical Statement and Animal Care
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Parameter | F3 (μg Protein/mL) | |||
---|---|---|---|---|
0.0 | 10 | 20 | 50 | |
Motility | +++++ | ++++ | ++ | + |
Agglutination | ++ | ++ | ++++ | +++++ |
F3 (μg Protein/mL) | Spermatozoa Status (%) | |
---|---|---|
Non-Capacitated | Capacitated | |
0.0 | 3.54 | 6.36 |
10 | 1.41 | 9.19 |
20 | 1.41 | 3.54 |
50 | 5.66 | 2.12 |
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Lazcano-Pérez, F.; Bermeo, K.; Castro, H.; Salazar Campos, Z.; Arenas, I.; Zavala-Moreno, A.; Chávez-Villela, S.N.; Jiménez, I.; Arreguín-Espinosa, R.; Fierro, R.; et al. A Sea Anemone Lebrunia neglecta Venom Fraction Decreases Boar Sperm Cells Capacitation: Possible Involvement of HVA Calcium Channels. Toxins 2022, 14, 261. https://doi.org/10.3390/toxins14040261
Lazcano-Pérez F, Bermeo K, Castro H, Salazar Campos Z, Arenas I, Zavala-Moreno A, Chávez-Villela SN, Jiménez I, Arreguín-Espinosa R, Fierro R, et al. A Sea Anemone Lebrunia neglecta Venom Fraction Decreases Boar Sperm Cells Capacitation: Possible Involvement of HVA Calcium Channels. Toxins. 2022; 14(4):261. https://doi.org/10.3390/toxins14040261
Chicago/Turabian StyleLazcano-Pérez, Fernando, Karina Bermeo, Héctor Castro, Zayil Salazar Campos, Isabel Arenas, Ariana Zavala-Moreno, Sheila Narayán Chávez-Villela, Irma Jiménez, Roberto Arreguín-Espinosa, Reyna Fierro, and et al. 2022. "A Sea Anemone Lebrunia neglecta Venom Fraction Decreases Boar Sperm Cells Capacitation: Possible Involvement of HVA Calcium Channels" Toxins 14, no. 4: 261. https://doi.org/10.3390/toxins14040261