Antagonistic Activity of Oroxylin A against Fusarium graminearum and Its Inhibitory Effect on Zearalenone Production
Abstract
:1. Introduction
2. Results
2.1. Qualitative and Quantitative Analyses of Oroxylin A in Piper Sarmentosum Extract (PSE)
2.2. Antifungal Activity of Oroxylin A against F. graminearum
2.3. Anti-Mycotoxin Activity of Oroxylin A against F. graminearum
2.4. Untargeted Metabolomic Analysis of Oroxylin A-Treated F. graminearum
2.5. Correlation Analysis between Differential Metabolites and Expression Changes of ZEB1 and ZEB2 Genes
3. Discussion
4. Conclusions
5. Materials and Methods
5.1. Chemicals and Reagents
5.2. Fungal Culture
5.3. Analysis of Oroxylin A in PSE via UPLC-QTOF-MS/MS
5.3.1. Preparation of Oroxylin A Standard Solution and PSE
5.3.2. UPLC-QTOF-MS/MS Analysis
5.4. Experimental Design
5.4.1. Antifungal Activity of Oroxylin A against F. graminearum
5.4.2. Anti-Mycotoxin Activity of Oroxylin A against F. graminearum
UPLC Analysis of ZEA
mRNA Expression Analysis of ZEA Biosynthesis Genes via RT-PCR
Metabolomic Analysis
5.5. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Zhou, L.; Hou, G.; Zhou, H.; Abouelezz, K.; Ye, Y.; Rao, J.; Guan, S.; Wang, D. Antagonistic Activity of Oroxylin A against Fusarium graminearum and Its Inhibitory Effect on Zearalenone Production. Toxins 2023, 15, 535. https://doi.org/10.3390/toxins15090535
Zhou L, Hou G, Zhou H, Abouelezz K, Ye Y, Rao J, Guan S, Wang D. Antagonistic Activity of Oroxylin A against Fusarium graminearum and Its Inhibitory Effect on Zearalenone Production. Toxins. 2023; 15(9):535. https://doi.org/10.3390/toxins15090535
Chicago/Turabian StyleZhou, Luli, Guanyu Hou, Hanlin Zhou, Khaled Abouelezz, Yuxiu Ye, Jun Rao, Song Guan, and Dingfa Wang. 2023. "Antagonistic Activity of Oroxylin A against Fusarium graminearum and Its Inhibitory Effect on Zearalenone Production" Toxins 15, no. 9: 535. https://doi.org/10.3390/toxins15090535