Environmental Toxin Biliatresone-Induced Biliary Atresia-like Abnormal Cilia and Bile Duct Cell Development of Human Liver Organoids
Abstract
:1. Introduction
2. Results
2.1. Biliatresone-Induced Aberrant Growth of Human Liver Organoids
2.2. Biliatresone-Induced Hepatocytic Differentiation of Human Liver Organoids
2.3. Biliatresone-Induced Apical-Basal Polarity Defect in Human Liver Organoids
2.4. Biliatresone Decreased the Number of Ciliated Cholangiocytes in Human Organoids
3. Discussion
4. Materials and Methods
4.1. Human Ethics
4.2. Human Liver Organoids
4.3. Treatment of Biliatresone
4.4. Measurement of Organoid Growth
4.5. Immunostaining and Confocal Imaging
4.6. Confocal Imaging and Analysis
4.7. Rhodamine 123 Transport Assay
4.8. FITC-Labeled Dextran Diffusion Tests
4.9. Calcium Imaging
4.10. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Hai-Bing, Y.; Sivasankaran, M.S.; Ottakandathil, B.R.; Zhong-Luan, W.; Man-Ting, S.; Ho-Yu, C.; Kak-Yuen, W.; Kwong-Hang, T.; Chi-Hang, L. Environmental Toxin Biliatresone-Induced Biliary Atresia-like Abnormal Cilia and Bile Duct Cell Development of Human Liver Organoids. Toxins 2024, 16, 144. https://doi.org/10.3390/toxins16030144
Hai-Bing Y, Sivasankaran MS, Ottakandathil BR, Zhong-Luan W, Man-Ting S, Ho-Yu C, Kak-Yuen W, Kwong-Hang T, Chi-Hang L. Environmental Toxin Biliatresone-Induced Biliary Atresia-like Abnormal Cilia and Bile Duct Cell Development of Human Liver Organoids. Toxins. 2024; 16(3):144. https://doi.org/10.3390/toxins16030144
Chicago/Turabian StyleHai-Bing, Yue, Menon Sudheer Sivasankaran, Babu Rosana Ottakandathil, Wu Zhong-Luan, So Man-Ting, Chung (Patrick) Ho-Yu, Wong (Kenneth) Kak-Yuen, Tam (Paul) Kwong-Hang, and Lui (Vincent) Chi-Hang. 2024. "Environmental Toxin Biliatresone-Induced Biliary Atresia-like Abnormal Cilia and Bile Duct Cell Development of Human Liver Organoids" Toxins 16, no. 3: 144. https://doi.org/10.3390/toxins16030144