In Vivo and In Vitro Interactions between Exopolysaccharides from Bacillus thuringensis HD270 and Vip3Aa11 Protein
Abstract
:1. Introduction
2. Results
2.1. Purification of EPS-HD270 and Vip3Aa11 Protein
2.2. Localization of Vip3Aa11 Protoxin and EPS-HD270 in the Midgut of Spodoptera frugiperda
2.3. Analysis of Vip3Aa11 Protoxin and Binding to Monosaccharides Contained in EPS-HD270
2.4. Effect of EPS-HD270 on the Insecticidal Activity of the Activated Vip3Aa11 Toxin
2.5. The Interaction of EPS-HD270 with Vip3Aa11 Protoxin and the Activated Toxin
2.6. Analysis of the Effect of EPS-HD270 on the Association of Activated Vip3Aa11 Protein with the BBMV of S. frugiperda
3. Discussion
4. Conclusions
5. Materials and Methods
5.1. Strains and Insect Culture
5.2. Expression and Purification of Insecticidal Protein
5.3. Extraction and Purification of EPS-HD270
5.4. Monosaccharides Bind to Vip3Aa11 Protoxin
5.5. Localization of Vip3Aa11 and EPS-HD270 in Spodoptera frugiperda
5.6. Preparation of BBMVs
5.7. Bioassay
5.8. ELISA Assay of the Binding Ability of Vip3Aa11 Protoxin and Activated Toxin to EPS-HD270
5.9. Western Blot Analysis of the Binding of Activated Vip3Aa11 toxin with EPS-HD270 to BBMVs of S. frugiperda
5.10. Data Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Ma, T.; Huang, J.; Xu, P.; Shu, C.; Wang, Z.; Geng, L.; Zhang, J. In Vivo and In Vitro Interactions between Exopolysaccharides from Bacillus thuringensis HD270 and Vip3Aa11 Protein. Toxins 2024, 16, 215. https://doi.org/10.3390/toxins16050215
Ma T, Huang J, Xu P, Shu C, Wang Z, Geng L, Zhang J. In Vivo and In Vitro Interactions between Exopolysaccharides from Bacillus thuringensis HD270 and Vip3Aa11 Protein. Toxins. 2024; 16(5):215. https://doi.org/10.3390/toxins16050215
Chicago/Turabian StyleMa, Tianjiao, Jinqiu Huang, Pengdan Xu, Changlong Shu, Zeyu Wang, Lili Geng, and Jie Zhang. 2024. "In Vivo and In Vitro Interactions between Exopolysaccharides from Bacillus thuringensis HD270 and Vip3Aa11 Protein" Toxins 16, no. 5: 215. https://doi.org/10.3390/toxins16050215