Isolation of the Binding Protein of Periplocoside E from BBMVs in Midgut of the Oriental Amyworm Mythimna separata Walker (Lepidoptera: Noctuidae) through Affinity Chromatography
Abstract
:1. Introduction
2. Results and Discussion
2.1. Identification of the Ligand (Periplocoside E-Semi-Succinic Acid Ester)
2.2. Isolation of Binding Proteins
3. Conclusions
4. Materials and Methods
4.1. Insects
4.2. Chemicals
4.3. Preparation of Brush Border Membrane Vesicles (BBMV)
4.4. Synthesis of Ligand
4.5. Preparation of the Medium and Coupling of the Ligand
4.6. Affinity Chromatography
4.7. SDS-PAGE and LC/Q-TOF-MS Analysis
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Number | Protein ID | Protein Mass | Protein |
---|---|---|---|
1 | gi|512918093 | 63095 | Luciferin 4-monooxygenase |
2 | gi|15212557 | 114719 | Aminopeptidase N |
3 | gi|21327773 | 107153 | Aminopeptidase N3 |
4 | gi|253807662 | 66764 | Nicotinamide adenine dinucleotide health (NADH) dehydrogenase subunit 5 |
5 | gi|520836887 | 78123 | Phosphatidylinositol 3-phosphate 3-phosphatase myotubularin |
6 | gi|323435326 | 31100 | Actin |
7 | gi|512901127 | 73062 | Uncharacterized family 31 glucosidase KIAA1161 |
8 | gi|399942986 | 86025 | 2OG-Fe(2) oxygenase superfamily protein |
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Feng, M.; He, Z.; Wang, Y.; Yan, X.; Zhang, J.; Hu, Z.; Wu, W. Isolation of the Binding Protein of Periplocoside E from BBMVs in Midgut of the Oriental Amyworm Mythimna separata Walker (Lepidoptera: Noctuidae) through Affinity Chromatography. Toxins 2016, 8, 139. https://doi.org/10.3390/toxins8050139
Feng M, He Z, Wang Y, Yan X, Zhang J, Hu Z, Wu W. Isolation of the Binding Protein of Periplocoside E from BBMVs in Midgut of the Oriental Amyworm Mythimna separata Walker (Lepidoptera: Noctuidae) through Affinity Chromatography. Toxins. 2016; 8(5):139. https://doi.org/10.3390/toxins8050139
Chicago/Turabian StyleFeng, Mingxing, Zhenyu He, Yuanyuan Wang, Xiufang Yan, Jiwen Zhang, Zhaonong Hu, and Wenjun Wu. 2016. "Isolation of the Binding Protein of Periplocoside E from BBMVs in Midgut of the Oriental Amyworm Mythimna separata Walker (Lepidoptera: Noctuidae) through Affinity Chromatography" Toxins 8, no. 5: 139. https://doi.org/10.3390/toxins8050139