On-Chip Construction of Multilayered Hydrogel Microtubes for Engineered Vascular-Like Microstructures
Abstract
:1. Introduction
2. Materials and Methods
2.1. A Four-Layer Microfluidic Device With Two Inlets for Introducing Multiple Solutions
2.2. Prepolymer Solution of Photo-Crosslinkable Hydrogels
2.3. Assembly of Alternating-Layered Microtubes
2.4. Assembly of Double-Layered Microtubes
2.5. Viability Evaluation of Cells Encapsulated Inside Microstructures
2.6. Data Acquisition and Analysis
3. Results and Discussion
3.1. Alternating-Layered Microtubes are Assembled by Controlling the Fabrication, Flow, and Assembling Sequences On-Chip
3.2. Optimized Diameter of Inner Layer Structures was Obtained for Assembling Double-Layered Microtubes
3.3. Square-Shaped Outer Layer Microstructures were UtiLized to Modify the Flow Conditions for Improving the Assembly of Double-Layered Microtubes
3.4. High-Efficient Assembly of Double-Layered Microtubes as Engineered Vascular-Like Microstructures
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Yue, T.; Liu, N.; Liu, Y.; Peng, Y.; Xie, S.; Luo, J.; Huang, Q.; Takeuchi, M.; Fukuda, T. On-Chip Construction of Multilayered Hydrogel Microtubes for Engineered Vascular-Like Microstructures. Micromachines 2019, 10, 840. https://doi.org/10.3390/mi10120840
Yue T, Liu N, Liu Y, Peng Y, Xie S, Luo J, Huang Q, Takeuchi M, Fukuda T. On-Chip Construction of Multilayered Hydrogel Microtubes for Engineered Vascular-Like Microstructures. Micromachines. 2019; 10(12):840. https://doi.org/10.3390/mi10120840
Chicago/Turabian StyleYue, Tao, Na Liu, Yuanyuan Liu, Yan Peng, Shaorong Xie, Jun Luo, Qiang Huang, Masaru Takeuchi, and Toshio Fukuda. 2019. "On-Chip Construction of Multilayered Hydrogel Microtubes for Engineered Vascular-Like Microstructures" Micromachines 10, no. 12: 840. https://doi.org/10.3390/mi10120840