An Optically Induced Dielectrophoresis (ODEP)-Based Microfluidic System for the Isolation of High-Purity CD45neg/EpCAMneg Cells from the Blood Samples of Cancer Patients—Demonstration and Initial Exploration of the Clinical Significance of These Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. Design of the ODEP Microfluidic System for High-Purity Isolation of CD45neg/EpCAMneg Cells
2.2. Microfabrication and Experimental Setup
2.3. The Working Scheme for the Isolation and Purification of CD45neg/EpCAMneg Cells
2.4. Working Mechanism and the Optimal Operating Conditions for the ODEP Force-Based Cell Manipulation in This Work
2.5. Evaluation of the CD45neg/EpCAMneg Cell Isolation Performance-Cancer Cell Line Model
2.6. Comparison of the Cancer-Related Gene Expression of the CD45neg/EpCAMneg Cell Populations Isolated from the Blood Samples of Healthy Donors and Head-and-Neck Cancer Patients
2.7. Statistical Analysis
3. Results and Discussions
3.1. Characteristic Features of the Proposed ODEP-Based Microfluidic System for the Isolation and Purification of CD45neg/EpCAMneg Cells
3.2. Optimal Operating Conditions for ODEP Force-Based Cell Manipulation
3.3. Performance Evaluation of CD45neg/EpCAMneg Cell Isolation
3.4. Comparison of the Cancer-Related Gene Expression of the CD45neg/EpCAMneg Cells Isolated from the Blood Samples of Healthy Donors and Head-and-Neck Cancer Patients
4. Conclusions
Supplementary Materials
Author Contributions
Acknowledgments
Conflicts of Interest
References
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Biological Function | Gene Name | Healthy Donor | Head-and-Neck Cancer Patient |
---|---|---|---|
Positive/Total | |||
EMT-related | EpCAM | 0/5 | 0/8 |
CK19 | 0/5 | 1/8 | |
Vimentin | 1/5 | 6/8 | |
SNAIL1 | 0/5 | 1/8 | |
MDR-related | MRP1 | 0/5 | 3/8 |
MRP2 | 0/5 | 0/8 | |
MRP4 | 0/5 | 0/8 | |
MRP5 | 0/5 | 2/8 | |
MRP7 | 0/5 | 0/8 | |
CSC-related | NANOG | 1/5 | 2/8 |
OCT4 | 1/5 | 4/8 |
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Liao, C.-J.; Hsieh, C.-H.; Chiu, T.-K.; Zhu, Y.-X.; Wang, H.-M.; Hung, F.-C.; Chou, W.-P.; Wu, M.-H. An Optically Induced Dielectrophoresis (ODEP)-Based Microfluidic System for the Isolation of High-Purity CD45neg/EpCAMneg Cells from the Blood Samples of Cancer Patients—Demonstration and Initial Exploration of the Clinical Significance of These Cells. Micromachines 2018, 9, 563. https://doi.org/10.3390/mi9110563
Liao C-J, Hsieh C-H, Chiu T-K, Zhu Y-X, Wang H-M, Hung F-C, Chou W-P, Wu M-H. An Optically Induced Dielectrophoresis (ODEP)-Based Microfluidic System for the Isolation of High-Purity CD45neg/EpCAMneg Cells from the Blood Samples of Cancer Patients—Demonstration and Initial Exploration of the Clinical Significance of These Cells. Micromachines. 2018; 9(11):563. https://doi.org/10.3390/mi9110563
Chicago/Turabian StyleLiao, Chia-Jung, Chia-Hsun Hsieh, Tzu-Keng Chiu, Yu-Xian Zhu, Hung-Ming Wang, Feng-Chun Hung, Wen-Pin Chou, and Min-Hsien Wu. 2018. "An Optically Induced Dielectrophoresis (ODEP)-Based Microfluidic System for the Isolation of High-Purity CD45neg/EpCAMneg Cells from the Blood Samples of Cancer Patients—Demonstration and Initial Exploration of the Clinical Significance of These Cells" Micromachines 9, no. 11: 563. https://doi.org/10.3390/mi9110563