Dual-Enzyme-Cascade Catalysis for PET Biodegradation Based on a Variable-Temperature Program
Abstract
:1. Introduction
2. Results and Discussion
2.1. The Properties of MuEst1-Based PET Degradation
2.2. MHETase Selection
2.3. Dual-Enzymatic Combination for PET Degradation
2.4. Catalytic Performance of Fusion Protein
2.5. Optimization of Degradation by Enzymatic Cascade
2.6. Application of the Two-Stage Variable-Temperature Program in a Scaled-Up System
3. Materials and Methods
3.1. Strains, Plasmids, and Reagents
3.2. Plasmid Construction
3.3. Preparation of Enzymes
3.4. Preparation of Substrate of PET Particles
3.5. Enzymatic-Activity Assay
3.6. PET-Degradation Process
4. Conclusions
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Lu, D.; Wu, J.; Jin, S.; Wu, Q.; Wang, F.; Deng, L.; Nie, K. Dual-Enzyme-Cascade Catalysis for PET Biodegradation Based on a Variable-Temperature Program. Catalysts 2024, 14, 543. https://doi.org/10.3390/catal14080543
Lu D, Wu J, Jin S, Wu Q, Wang F, Deng L, Nie K. Dual-Enzyme-Cascade Catalysis for PET Biodegradation Based on a Variable-Temperature Program. Catalysts. 2024; 14(8):543. https://doi.org/10.3390/catal14080543
Chicago/Turabian StyleLu, Dong, Jinglong Wu, Shuming Jin, Qiuyang Wu, Fang Wang, Li Deng, and Kaili Nie. 2024. "Dual-Enzyme-Cascade Catalysis for PET Biodegradation Based on a Variable-Temperature Program" Catalysts 14, no. 8: 543. https://doi.org/10.3390/catal14080543