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Volume 9
Issue 3
10.3390/catal9030250
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Article

The Synthesis of Mannose-6-Phosphate Using Polyphosphate-Dependent Mannose Kinase

by
Wenlong Zhu
,
Miaomiao Gao
,
Biqiang Chen
,
Tianwei Tan
,
Hui Cao
* and
Luo Liu
*
Beijing Bioprocess Key Laboratory, Beijing University of Chemical Technology, Beijing 100029, China
*
Authors to whom correspondence should be addressed.
Catalysts 2019, 9(3), 250; https://doi.org/10.3390/catal9030250
Submission received: 18 January 2019 / Revised: 5 March 2019 / Accepted: 6 March 2019 / Published: 9 March 2019
(This article belongs to the Special Issue Biocatalysis for Industrial Applications)
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Abstract

Mannose-6-phosphate (M6P) is involved in many metabolic pathways in life, and it has important applications in the treatment of diseases. This study explored a cost-effective enzyme catalytic synthesis method of M6P, using polyphosphate-dependent mannose kinase from Arthrobacter species. This synthesis uses polyphosphate to replace expensive ATP, and it is greener and safer than chemical synthesis. This study investigated the effects of key factors such as metal ions, temperature, and substrate addition on this enzymatic reaction, and improved the conversion efficiency. We moreover take advantage of the response surface method to explore the best catalytic conditions synthetically. The conversion was 99.17% successful under the optimal reaction conditions. After a series of optimizations, we carried out a 200 mL scale-up experiment, which proved that the method has good prospects for industrial applications.
Keywords: mannose-6-phosphate; polyphosphate-dependent mannose kinase; polyphosphate mannose-6-phosphate; polyphosphate-dependent mannose kinase; polyphosphate

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MDPI and ACS Style

Zhu, W.; Gao, M.; Chen, B.; Tan, T.; Cao, H.; Liu, L. The Synthesis of Mannose-6-Phosphate Using Polyphosphate-Dependent Mannose Kinase. Catalysts 2019, 9, 250. https://doi.org/10.3390/catal9030250

AMA Style

Zhu W, Gao M, Chen B, Tan T, Cao H, Liu L. The Synthesis of Mannose-6-Phosphate Using Polyphosphate-Dependent Mannose Kinase. Catalysts. 2019; 9(3):250. https://doi.org/10.3390/catal9030250

Chicago/Turabian Style

Zhu, Wenlong, Miaomiao Gao, Biqiang Chen, Tianwei Tan, Hui Cao, and Luo Liu. 2019. "The Synthesis of Mannose-6-Phosphate Using Polyphosphate-Dependent Mannose Kinase" Catalysts 9, no. 3: 250. https://doi.org/10.3390/catal9030250

APA Style

Zhu, W., Gao, M., Chen, B., Tan, T., Cao, H., & Liu, L. (2019). The Synthesis of Mannose-6-Phosphate Using Polyphosphate-Dependent Mannose Kinase. Catalysts, 9(3), 250. https://doi.org/10.3390/catal9030250

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