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Article

Fixed-Target Pink-Beam Serial Synchrotron Crystallography at Pohang Light Source II

1
Pohang Accelerator Laboratory, Pohang University of Science and Technology, Pohang 37673, Republic of Korea
2
College of General Education, Kookmin University, Seoul 02707, Republic of Korea
*
Author to whom correspondence should be addressed.
Crystals 2023, 13(11), 1544; https://doi.org/10.3390/cryst13111544
Submission received: 19 September 2023 / Revised: 9 October 2023 / Accepted: 26 October 2023 / Published: 27 October 2023
(This article belongs to the Special Issue X-Ray Protein Crystallography)

Abstract

Serial crystallography (SX) enables the determination of the structure of macromolecules or small molecules with minimal radiation damage. In particular, biomolecule structures determined using the SX technique have the advantage of providing room-temperature crystal structures with high biological relevance. The SX technique requires numerous crystals to be collected to complete three-dimensional structural information. To minimize crystal sample consumption, we introduced SX data collection with fixed-target (FT) pink-beam serial synchrotron crystallography (SSX) at the 1C beamline of Pohang Light Source II. A new sample holder consisting of a magnetic frame with a nylon mesh was developed for easy sample handling. The FT-pink-SSX diffraction data were collected by continuously scanning X-rays using a stepping motor. The room-temperature structures of glucose isomerase and lysozyme were successfully determined at a resolution of 1.7 and 2.2 Å, respectively. The use of pink-beam FT-SSX in experimental applications and data acquisition for large beam sizes is discussed. Our results provide useful information for future pink-beam SSX and SX data collection using large X-ray beams.
Keywords: serial crystallography; pink-beam; fixed-target scanning; room-temperature; synchrotron serial crystallography; pink-beam; fixed-target scanning; room-temperature; synchrotron

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MDPI and ACS Style

Kim, Y.; Nam, K.H. Fixed-Target Pink-Beam Serial Synchrotron Crystallography at Pohang Light Source II. Crystals 2023, 13, 1544. https://doi.org/10.3390/cryst13111544

AMA Style

Kim Y, Nam KH. Fixed-Target Pink-Beam Serial Synchrotron Crystallography at Pohang Light Source II. Crystals. 2023; 13(11):1544. https://doi.org/10.3390/cryst13111544

Chicago/Turabian Style

Kim, Yongsam, and Ki Hyun Nam. 2023. "Fixed-Target Pink-Beam Serial Synchrotron Crystallography at Pohang Light Source II" Crystals 13, no. 11: 1544. https://doi.org/10.3390/cryst13111544

APA Style

Kim, Y., & Nam, K. H. (2023). Fixed-Target Pink-Beam Serial Synchrotron Crystallography at Pohang Light Source II. Crystals, 13(11), 1544. https://doi.org/10.3390/cryst13111544

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