Double-Walled Poly-(D,L-lactide-co-glycolide) (PLGA) and Poly(L-lactide) (PLLA) Nanoparticles for the Sustained Release of Doxorubicin
Abstract
:1. Introduction
2. Experimental
2.1. Materials
2.2. Preparation of PLLA/PLGA Double-Walled Nanoparticles and PLGA Nanoparticles
2.3. Preparation of PLLA/PLGA Double-Walled Microparticles
2.4. Characterization of NP
2.4.1. Surface Morphology
2.4.2. Particles Size and Zeta Potential Analysis
2.4.3. Thermal Analysis by Differential Scanning Calorimetry (DSC)
2.4.4. Drug Encapsulation Efficiency (EE)
2.5. In Vitro DOX Release Studies
2.6. In Vitro Cell Assays
2.6.1. Cell Culture
2.6.2. Cell Viability Assays
- Flow Cytometry
- Absolute number of cells
- Fluorescence microscopy
3. Results and Discussion
3.1. Nanoparticles Characterization
3.2. In Vitro Controlled Drug Release
3.3. Viability Assays
4. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Particle | n | n Error (%) | k (day−1) | k Error (%) | r2 |
---|---|---|---|---|---|
DOX–DWNP 2:1 | 0.58 | 7.6 | 3.28 | 10.6 | 0.99 |
DOX–DWNP 4:1 | 0.49 | 10.1 | 2.98 | 11.3 | 0.99 |
DOX–DWNP 6:1 | 0.43 | 2.1 | 1.32 | 4.9 | 0.99 |
PLGA NP | 0.62 | 1.9 | 6.96 | 1.5 | 0.99 |
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Cardoso, M.M.; Peca, I.N.; Lopes, T.; Gardner, R.; Bicho, A. Double-Walled Poly-(D,L-lactide-co-glycolide) (PLGA) and Poly(L-lactide) (PLLA) Nanoparticles for the Sustained Release of Doxorubicin. Polymers 2021, 13, 3230. https://doi.org/10.3390/polym13193230
Cardoso MM, Peca IN, Lopes T, Gardner R, Bicho A. Double-Walled Poly-(D,L-lactide-co-glycolide) (PLGA) and Poly(L-lactide) (PLLA) Nanoparticles for the Sustained Release of Doxorubicin. Polymers. 2021; 13(19):3230. https://doi.org/10.3390/polym13193230
Chicago/Turabian StyleCardoso, M. Margarida, Inês N. Peca, Telma Lopes, Rui Gardner, and A. Bicho. 2021. "Double-Walled Poly-(D,L-lactide-co-glycolide) (PLGA) and Poly(L-lactide) (PLLA) Nanoparticles for the Sustained Release of Doxorubicin" Polymers 13, no. 19: 3230. https://doi.org/10.3390/polym13193230