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Polymers
Volume 14
Issue 19
10.3390/polym14193982
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Article

Polyhydroxybutyrate (PHB)-Based Biodegradable Polymer from Agromyces indicus: Enhanced Production, Characterization, and Optimization

by
Mohd Adnan
1,
Arif Jamal Siddiqui
1,
Syed Amir Ashraf
2,
Mejdi Snoussi
1,
Riadh Badraoui
1,
Mousa Alreshidi
1,3,
Abdelbaset Mohamed Elasbali
4,
Waleed Abu Al-Soud
5,
Salem Hussain Alharethi
6,
Manojkumar Sachidanandan
7 and
Mitesh Patel
8,*
1
Department of Biology, College of Science, University of Hail, Hail P.O. Box 2440, Saudi Arabia
2
Department of Clinical Nutrition, College of Applied Medical Sciences, University of Hail, Hail P.O. Box 2440, Saudi Arabia
3
Molecular Diagnostics and Personalized Therapeutics Unit, University of Hail, Hail P.O. Box 2440, Saudi Arabia
4
Department of Clinical Laboratory Science, College of Applied Sciences-Qurayyat, Jouf University, Sakaka P.O. Box 2014, Saudi Arabia
5
Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Jouf University, Sakaka P.O. Box 2014, Saudi Arabia
6
Department of Biological Science, College of Arts and Science, Najran University, Najran P.O. Box 1998, Saudi Arabia
7
Department of Oral Radiology, College of Dentistry, University of Hail, Hail P.O. Box 2440, Saudi Arabia
8
Department of Biotechnology, Parul Institute of Applied Sciences and Centre of Research for Development, Parul University, Vadodara 391760, India
*
Author to whom correspondence should be addressed.
Polymers 2022, 14(19), 3982; https://doi.org/10.3390/polym14193982
Submission received: 28 August 2022 / Revised: 16 September 2022 / Accepted: 18 September 2022 / Published: 23 September 2022
(This article belongs to the Special Issue Advances in Sustainable Polymers: Processing, Modeling, Properties and Applications)
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Abstract

Recently, there has been significant interest in bio-based degradable plastics owing to their potential as a green and sustainable alternative to synthetic plastics due to their biodegradable properties. Polyhydroxybutyrate (PHB) is a biodegradable polymer that is produced by bacteria and archaea as carbon and energy reserves. Due to its rapid degradation in natural environments, it can be considered a biodegradable plastic alternative. In the present study, a dye-based procedure was used to screen PHB-producing bacteria isolated from mangrove soil samples. Among the seven isolates, Agromyces indicus (A. indicus), identified by means of 16S rRNA analysis, accumulated the highest amount of PHB. The extracted polymer was characterized by a UV–Vis spectrophotometer, Fourier-transform infrared (FTIR) spectroscopy, and for the presence of the phbB gene, which confirmed the structure of the polymer as PHB. The maximum PHB production by A. indicus was achieved after 96 h of incubation at a pH of 8.0 and 35 °C in the presence of 2% NaCl, with glucose and peptone as the carbon and nitrogen sources, respectively. The strain was found to be capable of accumulating PHB when various cheap agricultural wastes, such as rice, barley, corn, and wheat bran, were used as the carbon sources. The response surface methodology (RSM) through the central composite design (CCD) for optimizing the PHB synthesis was found to be highly efficient at augmenting the polymer yields. As a result of the optimum conditions obtained from the RSM, this strain can increase the PHB content by approximately 1.4-fold when compared with an unoptimized medium, which would substantially lower the production cost. Therefore, the isolate A. indicus strain B2 may be regarded as one of the best candidates for the industrial production of PHB from agricultural wastes, and it can remove the environmental concerns associated with synthetic plastic.
Keywords: polyhyroxybutyrate; bioplastic; Agromyces indicus; response surface methodology; biodegradable polymer polyhyroxybutyrate; bioplastic; Agromyces indicus; response surface methodology; biodegradable polymer

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MDPI and ACS Style

Adnan, M.; Siddiqui, A.J.; Ashraf, S.A.; Snoussi, M.; Badraoui, R.; Alreshidi, M.; Elasbali, A.M.; Al-Soud, W.A.; Alharethi, S.H.; Sachidanandan, M.; et al. Polyhydroxybutyrate (PHB)-Based Biodegradable Polymer from Agromyces indicus: Enhanced Production, Characterization, and Optimization. Polymers 2022, 14, 3982. https://doi.org/10.3390/polym14193982

AMA Style

Adnan M, Siddiqui AJ, Ashraf SA, Snoussi M, Badraoui R, Alreshidi M, Elasbali AM, Al-Soud WA, Alharethi SH, Sachidanandan M, et al. Polyhydroxybutyrate (PHB)-Based Biodegradable Polymer from Agromyces indicus: Enhanced Production, Characterization, and Optimization. Polymers. 2022; 14(19):3982. https://doi.org/10.3390/polym14193982

Chicago/Turabian Style

Adnan, Mohd, Arif Jamal Siddiqui, Syed Amir Ashraf, Mejdi Snoussi, Riadh Badraoui, Mousa Alreshidi, Abdelbaset Mohamed Elasbali, Waleed Abu Al-Soud, Salem Hussain Alharethi, Manojkumar Sachidanandan, and et al. 2022. "Polyhydroxybutyrate (PHB)-Based Biodegradable Polymer from Agromyces indicus: Enhanced Production, Characterization, and Optimization" Polymers 14, no. 19: 3982. https://doi.org/10.3390/polym14193982

APA Style

Adnan, M., Siddiqui, A. J., Ashraf, S. A., Snoussi, M., Badraoui, R., Alreshidi, M., Elasbali, A. M., Al-Soud, W. A., Alharethi, S. H., Sachidanandan, M., & Patel, M. (2022). Polyhydroxybutyrate (PHB)-Based Biodegradable Polymer from Agromyces indicus: Enhanced Production, Characterization, and Optimization. Polymers, 14(19), 3982. https://doi.org/10.3390/polym14193982

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