Production of Graft Copolymers of Cod Collagen with Butyl Acrylate and Vinyl Butyl Ether in the Presence of Triethylborane—Prospects for Use in Regenerative Medicine
Abstract
:1. Introduction
- To adapt previously determined conditions for obtaining graft copolymers of collagen with methyl methacrylate (MMA) and acrylamide (AA) [18] using copolymer BA-VBE grafting;
- To characterize the target product using methods of physical and chemical analysis: gel permeation chromatography (GPC), scanning electron microscopy (SEM), elemental analysis (CHNS), and IR spectroscopy;
- To hydrolyze the CC-PBA-PVBE graft copolymer using collagenase, to isolate the grafted synthetic fragment, and to analyze it using GPC and Fourier-transform infrared spectroscopy (FTIR);
- To evaluate the cytotoxicity of the grafted copolymer using the MTT assay method.
2. Materials and Methods
2.1. Materials
2.2. Synthesis of the Graft Copolymer CC-PBA-PVBE
2.3. Enzymatic Hydrolysis
2.4. Gel-Penetrating Chromatography
2.5. FTIR Spectroscopy
2.6. Scanning Electron Microscopy
2.7. Elemental Analysis
2.8. Cytotoxicity Examination via MTT Assay
3. Results and Discussion
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Semenycheva, L.; Chasova, V.O.; Pegeev, N.L.; Uromicheva, M.A.; Mitin, A.V.; Kuznetsova, Y.L.; Farafontova, E.A.; Rubtsova, Y.P.; Linkova, D.D.; Egorikhina, M.N. Production of Graft Copolymers of Cod Collagen with Butyl Acrylate and Vinyl Butyl Ether in the Presence of Triethylborane—Prospects for Use in Regenerative Medicine. Polymers 2023, 15, 3159. https://doi.org/10.3390/polym15153159
Semenycheva L, Chasova VO, Pegeev NL, Uromicheva MA, Mitin AV, Kuznetsova YL, Farafontova EA, Rubtsova YP, Linkova DD, Egorikhina MN. Production of Graft Copolymers of Cod Collagen with Butyl Acrylate and Vinyl Butyl Ether in the Presence of Triethylborane—Prospects for Use in Regenerative Medicine. Polymers. 2023; 15(15):3159. https://doi.org/10.3390/polym15153159
Chicago/Turabian StyleSemenycheva, Lyudmila, Victoria O. Chasova, Nikita L. Pegeev, Marina A. Uromicheva, Alexander V. Mitin, Yulia L. Kuznetsova, Ekaterina A. Farafontova, Yulia P. Rubtsova, Daria D. Linkova, and Marfa N. Egorikhina. 2023. "Production of Graft Copolymers of Cod Collagen with Butyl Acrylate and Vinyl Butyl Ether in the Presence of Triethylborane—Prospects for Use in Regenerative Medicine" Polymers 15, no. 15: 3159. https://doi.org/10.3390/polym15153159