One-Pot Synthesis of Double-Network PEG/Collagen Hydrogel for Enhanced Adipogenic Differentiation and Retrieval of Adipose-Derived Stem Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Synthesis of Single-Crosslinked Hydrogels
2.2.1. PEG (NHS) Hydrogel
2.2.2. PEG (SMPT) Hydrogel
2.2.3. Collagen Hydrogel
2.3. One-Pot Synthesis of Double-Network Hydrogel
2.3.1. PEG (NHS)-Collagen Hydrogel
2.3.2. PEG (SMPT)-Collagen Hydrogel
2.4. Rheological Characterization of Hydrogel
2.5. Cell Culture and Encapsulation
2.6. Analysis of Cell Morphology and Cell Viability
2.7. Oil Red O Staining
2.8. QPCR Analysis
2.9. Cell Retrieval
2.10. Statistical Analysis
3. Results and Discussion
3.1. Fabrication of Hydrogels
3.2. Rheological Properties of Hydrogels
3.3. ADSC Culture within the Hydrogels
3.4. Adipogenic Differentiation in the Hydrogel
3.5. Cell Retrieval
4. Further Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene | Primer Sequences | |
---|---|---|
Β-actin | Sense | ACTACCTTCAACTCCATC |
Antisense | TGATCTTGATCTTCATTGTG | |
Oct4 | Sense | ACATCAAAGCTCTGCAGAAA |
Antisense | CTGAATACCTTCCCAAATAGAAC | |
Sox2 | Sense | TGCGAGCGCTGCACAT |
Antisense | GCAGCGTGTACTTATCCTTCTTCA | |
SSEA4 | Sense | TGGACGGGCACAACTTCATC |
Antisense | GGGCAGGTTCTTGGCACTCT | |
Adiponectin | Sense | TGGTGAGAAGGGTGAGAA |
Antisense | AGATCTTGGTAAAGCGAATG | |
aP2 | Sense | TGCAGCTTCCTTCTCACCTTGA |
Antisense | TCCTGGCCCAGTATGAAGGAAATC | |
PPAR-γ | Sense | ATGACAGCGACTTGGCAA |
Antisense | AATGTTGGCAGTGGCTCA |
Non-Degradable | Degradable | ||
---|---|---|---|
Single crosslink | Natural | Collagen | |
Synthetic | PEG (NHS) | PEG (SMPT) | |
Double crosslink | Hybrid | PEG (NHS)-Collagen | PEG (SMPT)-Collagen |
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Lee, H.; Hong, H.J.; Ahn, S.; Kim, D.; Kang, S.H.; Cho, K.; Koh, W.-G. One-Pot Synthesis of Double-Network PEG/Collagen Hydrogel for Enhanced Adipogenic Differentiation and Retrieval of Adipose-Derived Stem Cells. Polymers 2023, 15, 1777. https://doi.org/10.3390/polym15071777
Lee H, Hong HJ, Ahn S, Kim D, Kang SH, Cho K, Koh W-G. One-Pot Synthesis of Double-Network PEG/Collagen Hydrogel for Enhanced Adipogenic Differentiation and Retrieval of Adipose-Derived Stem Cells. Polymers. 2023; 15(7):1777. https://doi.org/10.3390/polym15071777
Chicago/Turabian StyleLee, Hwajung, Hye Jin Hong, Sujeong Ahn, Dohyun Kim, Shin Hyuk Kang, Kanghee Cho, and Won-Gun Koh. 2023. "One-Pot Synthesis of Double-Network PEG/Collagen Hydrogel for Enhanced Adipogenic Differentiation and Retrieval of Adipose-Derived Stem Cells" Polymers 15, no. 7: 1777. https://doi.org/10.3390/polym15071777