Preparation and Characterization of Molecularly Imprinted Polymer as SPE Sorbent for Melamine Isolation
Abstract
:1. Introduction
2. Experimental Section
2.1. Materials
2.2. Synthesis of Molecularly Imprinted Polymer (MIP)
2.3. Extraction of Melamine from Molecularly Imprinted Polymer (MIP)
2.4. Characterization of the Synthesized MIP
2.5. Adsorption Studies
3. Results and Discussion
3.1. Characterization of MIP
3.1.1. Fourier Transform Infrared Spectroscopy
3.1.2. Thermal Stability for MIP
3.1.3. Field Emission Scanning Electron Microscope (FESEM)
3.2. Adsorption Studies of MIP
3.2.1. pH Study
3.2.2. Effect of Dosage of MIP
3.2.3. Isotherm Study
Adsorption model | qe (exp.) (mg/g) | KL (mg/g) | KF (mg/g) | bL(L/mg) | bF (L/mg) | R2 |
---|---|---|---|---|---|---|
Langmuir constants | 6.5725 | 9.0909 | – | 0.035 | – | 0.802 |
Freundlich constants | – | 5.697 | – | 0.617 | 0.980 |
3.2.4. Kinetic Study
Kinetic model | qe (exp.) (mg/g) | qe (calc.) (mg/g) | k1 (min−1) | k2 (g∙mg−1∙ min−1) | R2 |
---|---|---|---|---|---|
First order | 53.01 | 2.702 | 0.061 | – | 0.239 |
Second order | 43.478 | – | 2.10 × 105 | 0.998 |
3.2.5. Selectivity Study
Compound | Kd MIP (mg/g) | Kd NIP (mg/g) | kMIP | kNIP | k′ |
---|---|---|---|---|---|
Melamine | 0.0061 | 0.0045 | 2.5337 | 0.8005 | 3.1651 |
Cyanuric acid | 0.0024 | 0.0057 |
4. Conclusions
Acknowledgments
Conflicts of Interest
References
- Lutter, P.; Savoy-Perroud, M.; Campos-Gimenez, E.; Meyer, L.; Goldmann, T.; Bertholet, M.; Mottier, P.; Desmarchelier, A.; Monard, E.; Perrin, C.; et al. Screening and confirmatory methods for the determination of melamine in cow’s milk and milk-based powdered infant formula: Validation and proficiency-tests of ELISA, HPLC-UV, GC-MS and LC-MS/MS. J. Food Control 2010, 22, 903–913. [Google Scholar]
- Venkatasami, G.; Sowa, J.R., Jr. A rapid, acetonitrile-free, HPLC method for determination of melamine in infant formula. Anal. Chim. Acta 2010, 665, 227–230. [Google Scholar] [CrossRef]
- Wu, Y.N.; Zu, Y.F.; Li, J.G. Melamine Analysis Group. A survey on occurrence of melamine and its analogues in tainted infant formula in china. Biomed. Environ. Sci. 2009, 22, 95–99. [Google Scholar] [CrossRef]
- Wang, C.; Qin, X.; Bo, H.; Fengqun, H.; Chengming, Z. Hemolysis of human erythrocytes induced by melamine–cyanurate complex. Biochem. Biophys. Res. Commun. 2010, 402, 773–777. [Google Scholar] [CrossRef]
- He, L.; Su, Y.; Zheng, Y.; Hung, X.; Wu, L.; Liu, Y.; Zeng, Z.; Chen, Z. Novel cyromazine imprinted polymer applied to the solid-phase extraction of melamine from feed and milk samples. J. Chromatogr. A 2009, 1216, 6196–6203. [Google Scholar]
- Xu, S.; Li, J.; Chen, L. Molecularly imprinted polymers by reversible addition-fragmentation chain transfer precipitation polymerization for preconcentration of atrazine in food matrices. Talanta 2011, 85, 282–289. [Google Scholar] [CrossRef]
- Song, X.; Li, J.; Wang, J.; Chen, L. Quercetin molecularly imprinted polymers: Preparation, recognition characteristics and properties as sorbent for solid-phase extraction. Talanta 2009, 80, 694–702. [Google Scholar] [CrossRef]
- He, C.; Long, Y.; Pan, J.; Li, K.; Liu, F. Application of molecularly imprinted polymers to solid-phase extraction of analytes from real samples. J. Biochem. Biophys. Methods 2007, 70, 133–150. [Google Scholar] [CrossRef]
- Li, M.; Zhang, L.; Meng, Z.; Wang, Z.; Wu, H. Molecularly-imprinted microspheres for selective extraction and determination of melamine in milk and feed using gas chromatography-mass spectrometry. J. Chromatogr. B 2010, 878, 2333–2338. [Google Scholar] [CrossRef]
- Yang, H.H.; Zhou, W.H.; Guo, X.C.; Chen, H.Q.; Zhao, H.Q. Molecularly imprinted polymer as SPE sorbent for selective extraction of melamine in dairy products. Talanta 2009, 80, 821–825. [Google Scholar] [CrossRef]
- Yan, H.; Row, K.H. Characteristic and synthetic approach of molecularly imprinted polymer. Int. J. Mol. Sci. 2006, 7, 155–178. [Google Scholar] [CrossRef]
- Komiyama, M.; Takeuchi, T.; Mukawa, T.; Asanuma, H. Molecular Imprinting from Fundamentals to Applications; WILEY-VCH verlag GmbH & Co. KGa A: Weinheim, Germany, 2003; pp. 9–17. [Google Scholar]
- Pan, G.; Zu, B.; Guo, X.; Zhang, Y.; Li, C.; Zhang, H. Preparation of molecularly imprinted polymer microspheres via reversible addition-fragmentation chain transfer precipitation polymerization. Polymer 2009, 50, 2819–2825. [Google Scholar]
- Dekhil, A.B.; Hannachi, Y.; Ghorbel, A.; Baubaker, T. Comparative study of the removal of cadmium from aqueos solution by low cost adsorbents. J. Environ. Sci. Technol. 2011, 4, 520–533. [Google Scholar] [CrossRef]
- Umpleby, R.J., II; Baxter, S.C.; Rampey, A.M.; Gregory, T.; Rushton, G.T.; Chen, Y.; Shimizu, K.D. Characterization of the heterogeneous binding site affinity distributions in molecularly imprinted polymers. J. Chromatogr. B 2004, 804, 141–149. [Google Scholar] [CrossRef]
- Ramstron, O.; Yan, M. Molecularly Imprinted Materials: Science and Technology; Marcel Dekker: New York, NY, USA, 2005; pp. 1–11. [Google Scholar]
- Ong, S.T.; Lee, C.K.; Zainal, Z. Removal of basic and reactive dyes using ethylenediamine modified rice hull. Bioresour. Technol. 2007, 98, 2792–2794. [Google Scholar] [CrossRef]
- Liu, Y.T.; Deng, J.; Xiao, X.L.; Ding, L.; Yuan, Y.L.; Li, H.; Li, X.T.; Yan, X.N.; Wang, L.L. Electrochemical sensor based on a poly(para-aminobenzoic acid) film modified glassy carbon electrode for the determination of melamine in milk. Electrochim. Acta 2011, 56, 4595–4602. [Google Scholar] [CrossRef]
- Zakaria, N.D.; Yusof, N.A.; Haron, J.; Abdullah, A.H. Synthesis and evaluation of a molecularly imprinted polymer for 2,4-dinitrophenol. Int. J. Mol. Sci. 2009, 10, 354–365. [Google Scholar] [CrossRef]
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Yusof, N.A.; Rahman, S.K.A.; Hussein, M.Z.; Ibrahim, N.A. Preparation and Characterization of Molecularly Imprinted Polymer as SPE Sorbent for Melamine Isolation. Polymers 2013, 5, 1215-1228. https://doi.org/10.3390/polym5041215
Yusof NA, Rahman SKA, Hussein MZ, Ibrahim NA. Preparation and Characterization of Molecularly Imprinted Polymer as SPE Sorbent for Melamine Isolation. Polymers. 2013; 5(4):1215-1228. https://doi.org/10.3390/polym5041215
Chicago/Turabian StyleYusof, Nor Azah, Siti Khadijah Ab. Rahman, Mohd Zobir Hussein, and Nor Azowa Ibrahim. 2013. "Preparation and Characterization of Molecularly Imprinted Polymer as SPE Sorbent for Melamine Isolation" Polymers 5, no. 4: 1215-1228. https://doi.org/10.3390/polym5041215