Synthesis of Mannosylated Polyethylenimine and Its Potential Application as Cell-Targeting Non-Viral Vector for Gene Therapy
Abstract
:1. Introduction
2. Experimental Section
2.1. Materials and Reagents
2.2. Synthesis of Man-PEI25k
2.3. Characterization of Copolymers
2.4. Characterization of the PEI/DNA Complexes
2.5. Gel Retardation Assay
2.6. Cytotoxicity Assay
2.7. Cell Uptake Study
2.8. In Vitro Transfection
2.9. Statistical Analysis
3. Results and Discussion
3.1. Synthesis and Characterization of Man-PEI25k
3.2. Characterization of Complexes
3.3. Gel Retardation Assays
3.4. Cytotoxicity Assay
3.5. Cell Uptake and Intracellular Distribution
3.6. Cell Transfection Assay
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Hu, Y.; Xu, B.-H.; Xu, J.-J.; Shou, D.; Gao, J.-Q. Synthesis of Mannosylated Polyethylenimine and Its Potential Application as Cell-Targeting Non-Viral Vector for Gene Therapy. Polymers 2014, 6, 2573-2587. https://doi.org/10.3390/polym6102573
Hu Y, Xu B-H, Xu J-J, Shou D, Gao J-Q. Synthesis of Mannosylated Polyethylenimine and Its Potential Application as Cell-Targeting Non-Viral Vector for Gene Therapy. Polymers. 2014; 6(10):2573-2587. https://doi.org/10.3390/polym6102573
Chicago/Turabian StyleHu, Ying, Bei-Hua Xu, Jiao-Jiao Xu, Dan Shou, and Jian-Qing Gao. 2014. "Synthesis of Mannosylated Polyethylenimine and Its Potential Application as Cell-Targeting Non-Viral Vector for Gene Therapy" Polymers 6, no. 10: 2573-2587. https://doi.org/10.3390/polym6102573