Evaluation of Electrospun PCL-PIBMD Meshes Modified with Plasmid Complexes in Vitro and in Vivo
Abstract
:1. Introduction
2. Experimental Section
2.1. Materials
2.2. Preparation of MPs and MPs-pZNF580 Complexes Suspension
2.3. Electrospinning Process
2.4. Characterization of Electrospun Fibrous Meshes
2.4.1. Analysis of the Surface Morphology of Electrospun Fibrous Meshes
2.4.2. FT-IR Measurements
2.4.3. Mechanical Properties Tests
2.4.4. Static Water Contact Angle
2.5. Biological Evaluation
2.5.1. Culture of EA.hy926 Cells
2.5.2. Cell Proliferation Assay
2.5.3. Fluorescence Assay
2.5.4. Platelet Adhesion Assay
2.5.5. Subcutaneous Implantation
2.6. Statistical Analysis
3. Results and Discussion
3.1. Morphology and Characterizations of Fibrous Meshes
3.2. ATR-FTIR Analysis
3.3. Mechanical Properties of Fibrous Meshes
3.4. WCA of Fibrous Meshes
3.5. Morphology of EA.hy926 Cells on PCL-PIBMD/MPs Meshes
3.6. Proliferation of EA.hy926 on PCL-PIBMD/MPs Meshes
3.7. Platelet Adhesion Examination
3.8. Tissue Response of Meshes in Subcutaneous Implantation
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Feng, Y.; Liu, W.; Ren, X.; Lu, W.; Guo, M.; Behl, M.; Lendlein, A.; Zhang, W. Evaluation of Electrospun PCL-PIBMD Meshes Modified with Plasmid Complexes in Vitro and in Vivo. Polymers 2016, 8, 58. https://doi.org/10.3390/polym8030058
Feng Y, Liu W, Ren X, Lu W, Guo M, Behl M, Lendlein A, Zhang W. Evaluation of Electrospun PCL-PIBMD Meshes Modified with Plasmid Complexes in Vitro and in Vivo. Polymers. 2016; 8(3):58. https://doi.org/10.3390/polym8030058
Chicago/Turabian StyleFeng, Yakai, Wen Liu, Xiangkui Ren, Wei Lu, Mengyang Guo, Marc Behl, Andreas Lendlein, and Wencheng Zhang. 2016. "Evaluation of Electrospun PCL-PIBMD Meshes Modified with Plasmid Complexes in Vitro and in Vivo" Polymers 8, no. 3: 58. https://doi.org/10.3390/polym8030058