Synthesis of Hybrid-Polypeptides m-PEO-b-poly(His-co-Gly) and m-PEO-b-poly(His-co-Ala) and Study of Their Structure and Aggregation. Influence of Hydrophobic Copolypeptides on the Properties of Poly(L-histidine)
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Synthesis of Nim-Trityl Protected N-Carboxyanhydride of L-Histidine (Trt-His-NCA)
2.3. Synthesis of N-Carboxy Anhydride of Glycine (Gly-NCA)
2.4. Synthesis of N-Carboxy Anhydride of L-Alanine (Ala-NCA)
2.5. Polymer Synthesis
2.6. Characterization
3. Results and Discussion
3.1. Synthesis
3.2. Secondary Structure
3.3. Aggregation Properties
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Polymer | Mn 1st Block × 10−3 a | Mn 2nd Block × 10−3 b | Total Mn × 10−3 c | I d | Composition % (w/w) e |
---|---|---|---|---|---|
m-PEO-b-poly(His-co-Gly) 10 | 9.95 | 6.20 | 16.1 | 1.13 | PEO/His/Gly: 62/36/2 |
m-PEO-b-poly(His-co-Gly) 20 | 9.95 | 5.75 | 15.6 | 1.11 | PEO/His/Gly: 62.1/34/3.9 |
m-PEO-b-poly(His-co-Gly) 40 | 9.95 | 6.10 | 15.9 | 1.15 | PEO/His/Gly: 62/30/8 |
m-PEO-b-poly(His-co-Ala) 10 | 9.95 | 6.30 | 16.1 | 1.10 | PEO/His/Ala: 62.8/35/2.2 |
m-PEO-b-poly(His-co-Ala) 20 | 9.95 | 6.0 | 16.1 | 1.09 | PEO/His/Ala: 62.6/33/4.4 |
m-PEO-b-poly (His-co-Ala) 40 | 9.95 | 6.30 | 16.2 | 1.14 | PEO/His/Ala: 62.7/27.5/9.8 |
Sample | pH = 7.4 (Filtration) | pH = 6.5 | pH = 5.0 | ||||||
---|---|---|---|---|---|---|---|---|---|
RH (nm) | Poly | KCounts | RH (nm) | Poly | KCounts | RH (nm) | Poly | KCounts | |
m-PEO-b-poly(His-co-Gly) 10 | 92.6 | 0.34 | 812 | 112.4 | 0.252 | 624 | 161.2 | 0.40 | 521 |
m-PEO-b-poly(His-co-Gly) 20 | 113.1 | 0.24 | 873 | 128.1 | 0.21 | 723 | 223 | 0.44 | 432 |
m-PEO-b-poly(His-co-Gly) 40 | 120.6 | 0.293 | 908 | 144.0 | 0.452 | 827 | 673.9 | 0.505 | 567 |
m-PEO-b-poly(His-co-Ala) 10 | 96.5 | 0.309 | 582 | 132 | 0.462 | 430 | 143.3 | 0.394 | 307 |
m-PEO-b-poly(His-co-Ala) 20 | 118.2 | 0.237 | 1013 | 163.7 | 0.416 | 908 | 165.3 | 0.36 | 497 |
m-PEO-b-poly(His-co-Ala) 40 | 111.5 | 0.23 | 761 | 121 | 0.309 | 714 | (1) 175.5 (2) 1005 | 0.467 | 647 |
Sample | pH = 7.4 (Filtration) | pH = 6.5 | pH = 5.0 | ||||||
---|---|---|---|---|---|---|---|---|---|
RG a | RH(0) b | RG/RH(0) | RG a | RH(0) b | RG/RH(0) | RG a | RH(0) b | RG/RH(0) | |
m-PEO-b-poly(His-co-Gly) 10 | 126.1 | 180.7 | 0.69 | 142.6 | 288.4 | 0.49 | 197.1 | 898.6 | 0.22 |
m-PEO-b-poly(His-co-Gly) 20 | 137.3 | 222.2 | 0.62 | 194.1 | 886.7 | 0.22 | 197.8 | 479.1 | 0.42 |
m-PEO-b-poly(His-co-Gly) 40 | 152.6 | 191.1 | 0.95 | 184.5 | 347.7 | 0.53 | 221.7 | 743.7 | 0.30 |
m-PEO-b-poly(His-co-Ala) 10 | 119.9 | 181.9 | 0.65 | 186.2 | 883.1 | 0.21 | 199 | 400.4 | 0.49 |
m-PEO-b-poly(His-co-Ala) 20 | 123.1 | 153.9 | 0.80 | 188.6 | 860.6 | 0.21 | 194.4 | 483.6 | 0.40 |
m-PEO-b-poly(His-co-Ala) 40 | 114.3 | 154.4 | 0.74 | 158.5 | 326.2 | 0.48 | 211.7 | 825.8 | 0.25 |
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Skoulas, D.; Stavroulaki, D.; Santorinaios, K.; Iatrou, H. Synthesis of Hybrid-Polypeptides m-PEO-b-poly(His-co-Gly) and m-PEO-b-poly(His-co-Ala) and Study of Their Structure and Aggregation. Influence of Hydrophobic Copolypeptides on the Properties of Poly(L-histidine). Polymers 2017, 9, 564. https://doi.org/10.3390/polym9110564
Skoulas D, Stavroulaki D, Santorinaios K, Iatrou H. Synthesis of Hybrid-Polypeptides m-PEO-b-poly(His-co-Gly) and m-PEO-b-poly(His-co-Ala) and Study of Their Structure and Aggregation. Influence of Hydrophobic Copolypeptides on the Properties of Poly(L-histidine). Polymers. 2017; 9(11):564. https://doi.org/10.3390/polym9110564
Chicago/Turabian StyleSkoulas, Dimitrios, Dimitra Stavroulaki, Konstantinos Santorinaios, and Hermis Iatrou. 2017. "Synthesis of Hybrid-Polypeptides m-PEO-b-poly(His-co-Gly) and m-PEO-b-poly(His-co-Ala) and Study of Their Structure and Aggregation. Influence of Hydrophobic Copolypeptides on the Properties of Poly(L-histidine)" Polymers 9, no. 11: 564. https://doi.org/10.3390/polym9110564