Efficiency of Marine Bacteria and Yeasts on the Biocontrol Activity of Pythium ultimum in Ancho-Type Pepper Seedlings
Abstract
:1. Introduction
2. Materials and Methods
2.1. Marine Antagonistic Microorganisms
2.2. Pythium Ultimum
2.3. Pathogenicity Test
2.4. Antagonistic Test
2.5. Detection of Lytic Activity
2.5.1. Phytopathogen Cells and Culture of Marine Microorganisms
2.5.2. β-1,3-glucanase Activity
2.6. Inoculation of Ancho-Type Pepper Plants with Marine Bacteria and Yeasts
2.7. Scanning Electron Microscopy Micrographs
2.8. Root Colonization of Marine Microorganisms
2.9. Statistical Analyses
3. Results
3.1. Pathogenicity of Pythium ultimum
3.2. In Vitro Radial Growth of Pythium Ultimum
3.3. Lytic Activity
3.4. Protection of Ancho-Type Pepper Plants by Marine Bacteria and Yeasts
3.5. Roots Colonized by Marine Microorganisms
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Microorganism | Strain | Lytic Activity | ||
---|---|---|---|---|
β-1,3-glucanase (U/mL) | ||||
12 h | 24 h | |||
Bacterium | S. rhizophila | KM01 | 27 d* | 41 e |
KM02 | 29 d | 47 d | ||
B. subtilis | RBM01 | 7 g | 17 g | |
RBM02 | 9 g | 14 g | ||
B. amyloliquefaciens | 2RLBF | 8 g | 15 g | |
3R4CF | 11 f | 21 fg | ||
Pseudomonas spp. | 2R6BF | 19 e | 40 e | |
2RE9CF | 20 e | 37 e | ||
Yeast | D. hansenii | 1R11AB | 2589 b | 5062 b |
1R11CB | 2105 c | 3562 c | ||
LL01 | 3907 a | 6062 a | ||
C. laurentii | 2R3BF | 21 e | 40 e | |
2R1CB | 19 e | 37 e |
Treatment | Height (cm) | Fresh Root Weight (g) | Dry Foliage Weight (g) | Dry Weight of Root (g) | Leaf Area (cm2) | Radical Volume (cm3) |
---|---|---|---|---|---|---|
Bacterium | ||||||
KM01 | 10.89 b | 3.62 b | 1.19 b | 1.03 b | 110.41 b | 28.37 b |
KM02 | 9.01 d | 1.16 e | 0.58 e | 0.51 d | 82.88 f | 20.59 d |
RBM01 | 10.71 b | 3.56 b | 1.18 b | 1.08 b | 107.75 c | 28.75 b |
RBM02 | 12.68 a | 4.22 a | 1.96 a | 1.51 a | 120.34 a | 35.62 a |
Yeast | ||||||
2R1CB | 9.98 c | 2.86 c | 0.82 c | 0.72 c | 95.33 d | 23.25 c |
LL01 | 9.74 c | 1.78 d | 0.75 d | 0.71 c | 90.81 e | 22.07 c |
1R11CB | 8.97 d | 1.22 e | 0.56 e | 0.52 d | 83.55 f | 17.37 e |
2R3BF | 8.85 d | 1.15 e | 0.56 e | 0.49 d | 80.32 g | 17.62 e |
Fungicide | 7.98 e | 0.81 f | 0.35 f | 0.22 e | 17.90 h | 6.75 f |
P. ultimum | 5.07 f | 0.49 g | 0.19 g | 0.11 f | 4.77 i | 3.11 g |
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Lara-Capistran, L.; Zulueta-Rodriguez, R.; Castellanos-Cervantes, T.; Reyes-Perez, J.J.; Preciado-Rangel, P.; Hernandez-Montiel, L.G. Efficiency of Marine Bacteria and Yeasts on the Biocontrol Activity of Pythium ultimum in Ancho-Type Pepper Seedlings. Agronomy 2020, 10, 408. https://doi.org/10.3390/agronomy10030408
Lara-Capistran L, Zulueta-Rodriguez R, Castellanos-Cervantes T, Reyes-Perez JJ, Preciado-Rangel P, Hernandez-Montiel LG. Efficiency of Marine Bacteria and Yeasts on the Biocontrol Activity of Pythium ultimum in Ancho-Type Pepper Seedlings. Agronomy. 2020; 10(3):408. https://doi.org/10.3390/agronomy10030408
Chicago/Turabian StyleLara-Capistran, Liliana, Ramon Zulueta-Rodriguez, Thelma Castellanos-Cervantes, Juan J. Reyes-Perez, Pablo Preciado-Rangel, and Luis G. Hernandez-Montiel. 2020. "Efficiency of Marine Bacteria and Yeasts on the Biocontrol Activity of Pythium ultimum in Ancho-Type Pepper Seedlings" Agronomy 10, no. 3: 408. https://doi.org/10.3390/agronomy10030408