Effect of N Supply Level and N Source Ratio on Cichorium spinosum L. Metabolism
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Material
2.2. Experimental Design
2.3. Gas Chromatography–Electron Impact–Mass Spectrometry (GC/EI/MS) Metabolomics Analysis of Cichorium spinosum L.
2.3.1. Chemicals and Reagents
2.3.2. Sampling and Extraction of Cichorium spinosum L. Leaves and Preparation for Metabolomics Analyses
2.3.3. Gas Chromatography–Electron Impact–Mass Spectrometry (GC/EI/MS) Analyses and Bioinformatics Analysis
3. Results
3.1. Overview of the GC/EI/MS Metabolomics Analysis
3.2. Impact of N Supply Level and N Source Ratio on the Amino Acid (AA) Pool of Stamnagathi
3.3. Impact of N Supply Level and N Source Ratio on Carbohydrate Content
3.4. Impact of N Supply Level and N Source Ratio on Carboxylic Acids, Fatty Acids, and Selected Stamnagathi Metabolites
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Chatzigianni, M.; Aliferis, K.A.; Ntatsi, G.; Savvas, D. Effect of N Supply Level and N Source Ratio on Cichorium spinosum L. Metabolism. Agronomy 2020, 10, 952. https://doi.org/10.3390/agronomy10070952
Chatzigianni M, Aliferis KA, Ntatsi G, Savvas D. Effect of N Supply Level and N Source Ratio on Cichorium spinosum L. Metabolism. Agronomy. 2020; 10(7):952. https://doi.org/10.3390/agronomy10070952
Chicago/Turabian StyleChatzigianni, Martina, Konstantinos A. Aliferis, Georgia Ntatsi, and Dimitrios Savvas. 2020. "Effect of N Supply Level and N Source Ratio on Cichorium spinosum L. Metabolism" Agronomy 10, no. 7: 952. https://doi.org/10.3390/agronomy10070952