The Utilization of Phosphogypsum as a Sustainable Phosphate-Based Fertilizer by Aspergillus niger

Abstract

Phosphogypsum (PG) is a by-product of the phosphorus (P) chemical industry, and usually contains ~2% residual phosphate. The P in PG would cause P pollution in the environment by the way of long-term stacking. Phosphate-solubilizing fungi (PSF) can effectively promote the release of P from insoluble phosphate. The aim of this study was to investigate the P release capacity between tricalcium phosphate (TCP) and PG by Aspergillus niger (ANG) via flask experiment. At the same time, the application of PG fertilizer (PGF) in soil was also performed. The dry biomass of ANG between TCP and PG treatment reached the maximum value of 0.71 and 0.38 g, respectively, after five days of incubation. The maximum P concentrations released from PG and TCP by ANG is five and seven days, i.e., 128 and 1103 mg/L, respectively. PG has a similar P release ratio compared with TCP by ANG, i.e., 52% vs. 55%. In the soil incubation experiment, PGF can significantly increase the soil available P content and continuously maintain the high concentration (17.2 mg/kg) after four weeks of incubation. The utilization of PG by ANG via the PGF is a feasible pathway to increase the soil P content, especially for the sustainable use of phosphate resources.

1. Introduction

Phosphate ore (PO) is the primary resource for the production of phosphoric acid, pesticides, and other phosphorus-related chemicals [1,2,3]. PO is critical to producing chemical phosphate fertilizer (CPF), especially in agricultural production [4]. The supply of CPF can provide phosphorus (P) for crop growth, which is important for food security and human development [4]. Every year, more than a million tons of PO is consumed in the effort of CPF production [5]. However, as a non-renewable resource, the huge consumption of PO would deplete current global reserves in the future [6].

Phosphogypsum (PG) is a by-product of CPF production via the wet-process phosphoric acid technology [7]. The current global production of PG is estimated to be 100~280 Mt per year [8]. PG is usually acidic (pH value 2–4) and could contain toxic elements (heavy metal cations, fluorine, etc.), which are hardly reused (less than 40%) [9]. Commonly PG is treated as a solid waste by way of land stockpiling [9,10]. However, the P in PG can be useful for crops [11]. The reuse of PG as a phosphate fertilizer can slow down the exploitation of PO with positive benefits for the environment [12,13]. The content of total P in PG is about 2.5–7.5% [14]. Besides soluble P and co-crystallized P, there is also insoluble P (IP) in PG due to the incompletely decomposed PO [15]. As with geological apatite, the IP in PG also has a low solubility (Ksp < 10⁻⁷⁰), which limits the reuse of P in PG [16].

Phosphate-solubilizing fungi (PSF) can secrete large amounts of organic acid and promote the release of P from IP via acidification and chelation [17,18]. Compared with phosphate-solubilizing bacteria, the P released from phosphate rocks by PSF is higher than 10 times [19]. Compared with chemical strong acids (e.g., sulfuric acid), the organic acid would be more effective in the release of P from phosphate rocks [20]. For example, oxalic acid has a higher P release ratio compared with sulfuric acid [20]. As a dicarboxylic acid, oxalic acid has the highest acidity constant (pK_a1 = 1.25 and pK_a2 = 4.27), which is effective in pH reduction and IP dissolution, especially for IP [18,21]. In addition, oxalic acid can also release P from phosphate rocks by chelating with metal cations, such as Ca²⁺, Al³⁺, Fe³⁺, etc. [11,17]. Therefore, PSF is usually considered as the primary candidate in the dissolution of phosphate rocks [21].

PSF inoculation with phosphate rocks has been applied for the production of phosphate-based bio-fertilizer [22]. In P deficient soil, the inoculation of PSF and phosphate rocks significantly increased the soil soluble P content and promoted plant growth [23,24,25]. Oxalic acid secreted by Aspergillus niger dominates the P dissolution process from phosphate rock in soil [23]. However, the secretion of oxalic acid by PSF can be influenced by different environmental factors, such as pH, heavy metal toxicity, IP types, etc. [21,26,27]. For example, the high Pb concentrations (1000 mg/L) limit the secretion of oxalic acid by Penicillium oxalicum (P. oxalicum) [28]. Hence, it is necessary to choose a suitable PSF to dissolve IP, especially for PG.

The PSF Aspergillus niger (A. niger) has a strong ability to secrete oxalic acid, even in a considerably stressed environment [28]. In a low pH (~2) and high Pb toxicity (500–1500 mg/L) environment, A. niger also maintains the high oxalic acid secretion ability [28,29]. In addition, A. niger can also resist the toxicity of fluorine (F) (~90 mg/L) and maintain the secretion of oxalic acid [30]. More importantly, the existence of calcium phosphate can also stimulate A. niger to secrete more oxalic acid [21]. Therefore, the application of A. niger to achieve the reuse of P in PG could be a feasible pathway.

This study aims to investigate the utilization of PG as a phosphate fertilizer with the combination of A. niger in a P deficient soil. The capacity of P released from tricalcium phosphate (TCP) and PG by A. niger is also investigated. High-performance liquid chromatography (HPLC) was used to analyze the secretion of organic acids. Inductively coupled plasma optical emission spectroscopy (ICP-OES) was used to analyze the release of P and Pb. Scanning electron microscopy (SEM) and X-ray diffraction (XRD) were used to identify mineral morphology in the process of PG utilization by A. niger.

2. Materials and Methods

2.1. Preparation of Fungal Strains

A. niger (NJDL-12, CGMCC No. 11544) was isolated from the soybean rhizosphere in Nanjing, China. Before incubation, A. niger was inoculated into a potato dextrose agar medium (PDA) in a sterilized environment. After incubating for five days at 28 °C, the formed spores were collected into an Erlenmeyer flask with sterile water and filtered through three layers of sterile gauze to obtain a spore suspension. Then, a hemocytometer was used to measure the concentration of spores to 10⁷ CFU/mL.

2.2. Medium Preparation

Pikovskaya (PVK) liquid medium was used in this study to investigate the P-releasing ability of A. niger. The PVK medium contained 10 g glucose, 0.2 g NaCl, 0.25 g MgSO₄·7H₂O, 0.03 g FeSO₄·7H₂O, 0.03 g MnSO₄·4H₂O, 0.2 g KCl, 0.5 g (NH₄)₂SO₄, and 1 L deionized water (China National Pharmaceutical Group Chemical Reagent Co., Ltd., Shanghai, China). Two phosphates were used as the initial P resources in the PVK medium, i.e., TCP and PG [21].

2.3. TCP, PG and Soil Collection

Tricalcium phosphate (Ca₃(PO₄)₂) was supplied by Shanghai Macklin Biochemical Co., Shanghai, China. PG was collected from the phosphorus chemical industry in Tongling city, Anhui Province, China. The total P content in TCP and PG was 2 g/kg 24.81 mg/kg. The collected PG was air-dried and passed through a 20-mesh sieve. The soil was collected from the North Anhui comprehensive experimental station in Suzhou city, Anhui Province, China. The physical and chemical properties of the soil are shown in Table S3. Before incubation, plant roots and gravel were removed from the soil. All soil samples were ventilated and dried at room temperature, then ground and passed through a 20-mesh sieve. After that, the soil samples were sterilized for 20 min in a 121 °C steam sterilization pot.

2.4. PG Fertilizer Preparation

The generation of PG fertilizer (PGF) for A. niger was performed by shallow disk fermentation. Before the fermentation, A. niger was cultured on potato dextrose agar (PDA) medium for five days at 28 °C to form spores. Then, the block of A. niger containing spores was inoculated to potato dextrose broth (PDB) at 28 °C and 175 rpm for three days to form a fungal solution. The PGF materials consisted of corn stalk, wheat stalk, PG, rice stalk, soybean powder in a ratio of 8:2:8:1:1. After the sterilization (121 °C, 20 min), 2.5 kg materials and 3.0 kg fungal solution (ratio 1:1.2) were uniformly mixed by glass rod in a steel pallet. Lastly, the mixture materials were covered with two layers of gauze to continue incubation at 28 °C for one week, during which the following plot experiment was prepared.

2.5. Flask Experiment

The experiment was performed with two treatments, i.e., ANG + TCP (A. niger + Ca₃(PO₄)₂) and ANG + PG (A. niger + PG). Before incubation, 100 mL PVK medium was added into a 250 mL conical flask and sterilized at 121 °C for 20 min [21]. Then, 1.0 g Ca₃(PO₄)₂ was added to one flask and 1.0 g PG was added to the second flask. Subsequently, 1 mL of ANG spore suspension was added to each conical flask [21]. Finally, the conical flasks were sealed with a parafilm (BS-QM-003, Biosharp), which contained a 0.22 μm air breathable membrane in the middle [21]. All operations were performed under aseptic conditions. After being incubated for three, five, and seven days (28 °C, 180 rpm), the PVK medium was filtered using a 0.22 μm polyethersulfone (PES) membrane [21]. The filtrate was used to determine pH, organic acids, and soluble P. After being dried at 65 °C for 24 h, the filter was analyzed by XRD and SEM to determine the minerals [21]. The fungi obtained by filtration was dried at 65 °C for 24 h to determine biomass and was analyzed by XRD and SEM [21]. All treatments were replicated three times.

2.6. Soil Incubation Experiment

There are five treatments in this experiment, i.e., SS (sterilized soil only), SS + ANG (sterilized soil + A. niger), SS + PG (sterilized soil + PG), SS + ANG + PG (sterilized soil + A. niger + PG) and SS + PGF (sterilized soil + PG fertilizer). The soil was sterilized three times at 121 °C for 20 min before incubation. In each treatment, 20 g sterilized soil was added into a 150 mL white bottle before incubation. The inoculation amount of PG in soil was 1 g, and the amount of PGF was 2 g. Before incubation, the PG and PGF were completely mixed with the soil. The inoculation of A. niger was 2 mL. All bottles were incubated in an incubator at 30 °C. After being incubated for one, two, and four weeks, the soil in each treatment was collected and measured for pH and water-soluble P. In the soil pH test, the weight ratio of soil and water was 1:2.5. Before the water-soluble P test, 2 g soil was mixed with 40 mL deionized water and shaken at 180 rpm and 25 °C for 18 h. Then, the mixture liquid was centrifuged at 4000 rpm for 10 min and then stranded for 30 min. Finally, the supernatant was filtered via a 0.22 μm filter for the water-soluble P test [23,31]. The morphology of PGF in soil was also analyzed by SEM. All treatments were replicated three times.

2.7. Instrumentation

The pH value was measured by pH meter (FE20, Mettler Toledo, Columbus, OH, USA). The concentration of P was analyzed by ICP-OES (iCAP 7000 series, Thermo Fisher Scientific, Waltham, MA, USA). Calibration curves (1, 5, 10, 20, and 50 mg/L) were prepared using phosphorus standards (non-ferrous metals and electronic materials testing).

HPLC (Agilent 1200, Agilent Technologies, Santa Clara, CA, USA) was used to determine the content of organic acids. The HPLC column temperature was 30 °C. The mobile phase included 2.5 wt‰ potassium dihydrogen phosphate (KH₂PO₄) and methanol (CH₃OH) in a ratio of 99:1. Phosphoric acid was used to adjust the pH of KH₂PO₄ to 2.8 at a rate of 1 mL/min. Oxalic acid and citric acid standard solutions were diluted to 2000, 1000, 500, 200, 100, 50, and 0 mg/L [28].

D/Max-2500 X-ray diffraction (XRD, Rigaku Corporation, Tokyo, Japan) (Cu-Kα; 36 kV; 20 mA; scanning from 5° to 60° at a speed of 4°/sec) was used to analyze the precipitates. Before XRD analysis, the filtered thalli were dried in an oven, and then dry precipitated in a planetary ball mill (Mitr YXQM, Changsha Mitrcn Instrument Equipment Co., Ltd. (MITR), Changsha, China) for ball grinding and passing through a 100-mesh sieve [21]. MDI Jade 6.5 software was used to detect the results of XRD precipitation for phase identification. Scanning electron microscopy (SEM, S4800 Hitachi, Hitachi High-Technologies Corporation, Tokyo, Japan) was used to study the surface morphology of the samples at 7 kv accelerating voltage. Before the test, the sample was coated with a layer of gold in Hitachi E-1010 Sputter for one minute. The semi-quantitative analysis was performed by X-Max N 150 energy dispersive spectrometer (EDS, Oxford Instruments, Abingdon-on-Thames, UK) at 10 kv accelerating voltage.

2.8. Statistical Analysis

The flask and soil incubation experiments were performed in triplicate. The means and standard deviations in each treatment were calculated and presented. The significant differences among the treatments were identified by Tukey’s honestly significant difference test (p < 0.05) via one-way ANOVA. XRD and SEM analyses were carried out on a range of samples, and the typical results are presented in this study.

3. Results

3.1. Fungal Biomass, pH, and P Content Released by A. niger in Flask Experiment

The dry biomass of ANG in ANG + TCP treatment was significantly higher than in ANG + PG treatment (Figure 1A). After three days of incubation, the dry biomass in ANG + TCP treatment was 0.58 g (Figure 1A). The dry biomass of ANG in ANG + TCP treatment then increased to 0.70 and 0.71 g on days five and seven (Figure 1A). In ANG + PG treatment, the dry biomass of ANG was 0.34, 0.38, and 0.37 g after three, five, and seven days of incubation (Figure 1A).

The initial pH of the PVK medium was 6.5. In ANG + TCP treatment, the pH value was 3.02 on day three, significantly higher than the value of 2.52 in ANG + PG treatment (Figure 1B). However, the pH value had no significant difference between ANG + TCP and ANG + PG treatments on day five, i.e., 2.30 and 2.41 (Figure 1B). After seven days of incubation, the pH value in ANG + PG treatment was 2.72, significantly higher than the value of 2.41 in ANG + TCP treatment (Figure 1B).

The P concentration in ANG + TCP treatment was ~10 times higher than ANG + PG treatment during the incubation time (Figure 2A). In ANG + TCP treatment, the P concentration was 779.7, 801.6, and 1103.1 mg/L after three, five, and seven days of incubation (Figure 2A). In ANG + PG treatments, the P concentration was 66.5, 128.2, and 118.2 mg/L after three, five, and seven days of incubation (Figure 2A). The maximum P release ratio has a similar value (~55%) between ANG + TCP and ANG + PG treatments (Figure 2B). In ANG + TCP treatment, the release ratio of P continuously increased to 39.2%, 40.2%, and 55.3% after three, five, and seven days of incubation (Figure 2B). In ANG + PG treatments, the release ratio of P was 27.3%, 52.1%, and 48.1% after three, five, and seven days of incubation, and reached the maximum on day three (Figure 2B).

3.2. Secretion of Organic Acids by A. niger in Flask Experiment

Oxalic and citric acids were the two primary organic acids secreted by A. niger (Figure 3). The amount of oxalic acid secreted by A. niger in ANG + TCP treatment was significantly higher than in ANG + PG treatment during the whole incubation time (Figure 3A). In ANG + TCP treatment, the concentration of oxalic acid was 252.5 mg/L on day three and then decreased sharply to 65.2 and 74.5 mg/L on days five and seven (Figure 3A). In ANG + PG treatment, the oxalic acid secreted by A. niger was gradually increased from 11.9 to 14.5 to 23.9 mg/L after three, five, and seven days of incubation, respectively (Figure 3A). In contrast, the secretion of citric acid by A. niger in ANG + TCP treatment was significantly higher than ANG + PG treatment (Figure 3). In ANG + TCP treatment, the secretion of citric acid by A. niger decreased from 46.5 to 4.4 to 2.0 mg/L after three, five, and seven days of incubation, respectively (Figure 3B). In ANG + PG treatment, the secretion of citric acid was 11.6, 59.2, and 57.2 mg/L after three, five, and seven days of incubation, respectively (Figure 3B).

3.3. XRD Analysis

XRD patterns show the minerals present in different treatments after seven days of incubation (Figure 4). In ANG + TCP treatment, the peaks at 29.4 and 31.0° represented tricalcium phosphate (Ca₃(PO₄)₂) (Figure 4). The new peaks located at 14.3, 23.7 and 37.4° in ANG + TCP treatment indicated the existence of calcium oxalate (CaC₂O₄) (Figure 4). In ANG+PG treatment, the Ca₃(PO₄)₂ peaks were detected at 25.8, 28.3 and 31.0°. The new CaC₂O₄ peaks were detected in ANG+PG treatment at 14.3 and 23.7°. The new mineral calcium sulfate (CaSO₄) appeared in ANG+PG treatment at a peak of 10.9°. In addition, the new lead oxalate (PbC₂O₄) peak was observed at 32.4° in ANG+PG treatment.

3.4. Soil pH and Soil Soluble P Content

The initial pH value in soil was 7.8 (Figure 5A). After one, two, and four weeks of incubation, the pH values in SS treatment were 7.8, 7.8, and 7.6, respectively (Figure 5A). SS + ANG treatment decreased the pH value in the soil, and the pH values were 7.4, 7.4, 7.4 on weeks one, two, and four, respectively (Figure 5A). The pH value in SS+PG, SS+ANG+PG, and SS+PGF treatments decreased significantly (Figure 5A). After one and two weeks of incubation, the pH values in SS + PG treatment (6.9 and 6.9) were lower than SS + ANG + PG treatment (7.1 and 7.0) (Figure 5A). After four weeks of incubation, the pH value in SS + PG treatment (6.9) was higher than SS + ANG + PG treatment (6.6) (Figure 5A). The pH value in SS + PGF treatment decreased mostly (Figure 5A). In SS + PGF treatment, the pH values were 6.7, 6.6, and 6.6 after one, two, and four weeks of incubation, respectively (Figure 5A).

The initial water-soluble P in soil was 0.9 mg/kg. The P concentrations in SS + PG, SS + ANG + PG, and SS + PGF treatments were significantly higher than SS and SS + ANG treatments (Figure 5B). In SS and SS + ANG treatments, the P concentrations were 1.0, 0.2, 0.8, and 2.2, 0.5, and 1.3 mg/kg after one, two, and four weeks of incubation, respectively (Figure 5B). After one week of incubation, the P concentration in SS + PG and SS + ANG + PG treatments were 22.2 and 21.9 mg/kg, which was higher than SS + PGF treatment (15.6 mg/kg) (Figure 5B). After two weeks of incubation, the P concentration in SS + PG, SS + ANG + PG, and SS + PGF treatments showed no significant difference but decreased to 10.3, 14.2, and 10.0 mg/kg, respectively (Figure 5B). After four weeks of incubation, the P concentrations in SS + PG and SS + ANG + PG treatments were significantly lower than SS + PGF treatment, which were 11.3, 12.5, and 17.2 mg/kg, respectively (Figure 5B). Compared with PG, the inoculation of PGF increased 52.2% water-soluble P content in soil after the incubation (Figure 5B). PG fertilizer can continuously increase the content of available P in soil compared with PG and PG + ANG treatment.

3.5. SEM-EDS Analysis

The SEM images show mineral and fungal morphology between ANG + TCP and ANG + PG treatments after seven days of incubation in the flask experiment (Figure 6). A. niger hypha can be observed in these two treatments (Figure 6). The mineral calcium oxalate, which has a prismatic crystal shape, was identified in ANG + TCP treatment (Figure 6A). In ANG + PG treatment, both tricalcium phosphate and calcium oxalate minerals were also observed (Figure 6B).

The SEM images showed the morphology of minerals and fungi in PGF and SS + PGF treatment after four weeks of incubation in the soil incubation experiment (Figure 7). In PG fertilizer (PGF), both A. niger (hyphae and globular spores) and minerals (calcium oxalate and calcium sulfate) were observed (Figure 7A). In SS + PGF treatment, the morphology of soil block, crop straw, fungal hypha and spores were also clearly observed (Figure 7B).

4. Discussion

PG is a by-product of wet-process phosphoric acid production [7], with a low pH (2–4) and P content (~2%) [9]. Although PG has a low P content compared with TCP (24.8 mg/g in PG vs. 200 mg/g in TCP) (Table S1), the P in PG is more easily released by A. niger (Figure 2). More importantly, the maximum P release ratio in PG is similar to TCP (~55%), but the time to achieve this ratio is shortened to five days (Figure 2B). On the one hand, PG is digested by strong chemical acid during phosphoric production and, hence, contains part of P with high solubility, which is easy to release [32]. Our results indicated that the water-soluble P content in PG accounts for 14.7% of total P (Table S1). On the other hand, P in PG also exists in the form of eutectic P (CaHPO₄·H₂O and CaSO₄·2H₂O), which is also easier to release than Ca₃(PO₄)₂ (the solubility of eutectic P is greater than Ca₃(PO₄)₂, i.e., 0.32 g/L [CaHPO₄] vs. 0.02 g/L [Ca₃(PO₄)₂]) [33,34,35].

Untreated PG also could contain toxic elements, such as heavy metal and fluorine, depending on the grade of the PO being processed [8]. These toxic elements could limit the growth of microbes and cause environmental pollution. However, A. niger still survives in PG treatment (Figure 1). Compared with other fungi (e.g., Penicillium oxalicum), A. niger has a higher tolerance to Pb (1500 mg/L) and fluorine (90 mg/L) toxicity [28,30]. A. niger also maintains a high secretion ability of organic acids, especially in low pH (~2) and high Pb toxicity (500–1500 mg/L) environments [28,30]. Our results also confirmed that A. niger can secrete oxalic and citric acids in PG treatment. This organic acid secreted by A. niger contributes to P release and Pb remediation. Although the amount of oxalic acid secreted by A. niger is less than citric acid (20 vs. 60 mg/L), the formed calcium oxalate in ANG + PG treatment indicated that oxalic acid dominated the release of P from PG. With no A. niger treatment, both TCP and PG release P content is much lower than that with A. niger treatment (Table S2). In addition, the common mineral of lead oxalate was also detected in ANG + PG treatment (Figure 4), which also indicated that the release of Pb from PG can be easily immobilized by oxalic acid [36].

Organic acids secreted by A. niger can promote the release of P through chelation and acidification [17,18], especially oxalic acid, which has a higher chelating ability and acidity constant, and usually dominates the dissolution of IPs [20]. Our XRD and SEM results also showed that oxalic acid dominated the dissolution of TCP and PG via the formation of calcium oxalate (Figure 4 and Figure 6). The existence of Ca²⁺ can stimulate A. niger to secrete more oxalic acid [21]. However, the main component of calcium sulfate (CaSO₄) in PG has a lower solubility compared with TCP (less than 0.002 g/L) [37]. Hence, the secretion of oxalic acid by A. niger in TCP is higher than PG (Figure 3A). However, after five and seven days of incubation, oxalic acid in ANG + PG treatment showed an increasing trend (Figure 3). PG can stimulate A. niger to continuously increase the secretion of oxalic acid, which is important for the decomposition of IPs. Therefore, although TCP releases more P, it does not promote P release efficiency compared with PG (Figure 2).

A. niger promoted the release of P in PG under liquid culture conditions. PG could only release 28 mg/L P without A. niger, but it increased to 128 mg/L after adding A. niger (Table S2 and Figure 2). PG in soil can also rapidly increase soil P content (22.2 mg/kg), but released P tended to be fixed (down to 10.3 mg/kg in the second week) (Figure 5B). Although PG has a higher P content in soil compared with PG fertilizer (PGF) at beginning of the soil incubation, PGF can continuously increase soil P content (Figure 5B). Unlike PG, the component of PGF is more complex, and the existence of straw can also adsorb part of P. In addition, A. niger also has cellulase activity, which can promote the degradation of crop straw cellulose and thus increase the source of P in PGF [38,39]. Therefore, with the increased time of soil incubation, PGF shows a higher P content in soil (Figure 5B). In addition, A. niger has strong environmental resistance, and the mycelia extended by spores can be seen by SEM (Figure 7). The application of PGF reduces the pH of soil (Figure 5A), and can be used in saline-alkali land areas [40]. Ca²⁺ in PGF can replace Na⁺ in soil colloid to achieve a desalination effect [41]. The use of PGF can also increase the content of some trace elements in soil, thus promoting the growth of plants [40]. Importantly, PGF application has been tested for the risk of lead-free contamination of soil. Although PG partly contended lead cations, the secretion of oxalic acid by A. niger can also immobilize Pb, which has a low environmental risk.

5. Conclusions

PG is strongly acidic and could contain harmful elements such as Pb and F, which carry environmental risk. A. niger can survive in a PG environment, not only promoting the release of phosphorus from PG, but also reducing environmental risk via the formation of lead oxalate. Compared with high phosphorus content phosphate (TCP), the phosphorus is easily and quickly released from PG by A. niger. Both PG and PG fertilizer can significantly increase the P concentration in soil without heavy metal pollution. However, the use of PG fertilizer can continuously improve the available phosphorus content in soil. In addition, the oxalic acid secreted by A. niger dominated the release of phosphorus from PG. The dissolution of PG in soil by A. niger in the future should be accompanied by increased oxalic acid secretion. In conclusion, the combination of A. niger and PG for PG fertilizer is a feasible pathway for the reuse of PG, which can reduce the consumption of phosphate ore resources.