An Approach for Obtaining Stable, Reproducible, and Accurate Fibrogram Measurements from High Volume Instruments

Round 1

Reviewer 1 Report

The authors present a manuscript detailing a procedure to make fibrogram measurement reproducible.The manuscript is well written and readily understood. The language used is very good. Overall the presented research could be valuable to other researchers in the field of fiber measurements.  The researchers presented a good introduction of the problem and previous work by other researchers. The researchers were very careful in describing the experimental part. Therefore, these experiments could be replicated by other researchers familiar with the area. The conclusions in this manuscript are supported by the experimental results. Thus I support the publication of this manuscript.

Author Response

Dear reviewer

We appreciate you for the time in reviewing the manuscript and thank you for the comments. Please kindly see our response in the attachment.

Thanks!

Reviewer 2 Report

I have made my comments in the body of the manuscript. It needs a bit of tightening up prior to publication. Reference section needs to be improved as does some of the figures.

Comments for author File: Comments.pdf

Author Response

Dear reviewer

We appreciate you for the time in reviewing the manuscript and thank you for the comments. We have been able to address and incorporate most of your comments and edits point-by-point. Changes made in the revised manuscript are marked using track changes. Please kindly see our response in the attachment.

Thanks!

Author Response File: Author Response.pdf

Reviewer 3 Report

The authers investigated the stability of the fibrogram and the reproducibility across multiple instruments. The results indicated that for a given HVI, the entire fibrogram is stable over both short-term and long-term. There were differences among HVIs and the proposed correction procedure can effectively reduce the differences among HVIs. This result could help identify important span lengths extracted from a fibrogram that better capture the variability within the sample than the length parameters currently reported. I think that this manuscript is acceptable. I have only one advice. It should be better for more reader to compare the fiber length from HVI (especially after correction by proposed method) and combed fiber by hand in the part of result or discussion. 

Author Response

Dear reviewer

We appreciate you for the time in reviewing the manuscript and thank you for the comments. Please kindly see our response in the attachment.

Thanks!

Author Response File: Author Response.pdf