Low Temperature Rather Than Nitrogen Application Mainly Modulates the Floral Initiation of Different Ecotypes of Rapeseed (Brassica napus L.)
Abstract
:1. Introduction
2. Materials and Methods
2.1. Experiment 1: Floral Initiation Response to Soluble Sugar and Protein Levels in the Leaf
2.2. Experiment 2: Transcriptional Regulation of Floral Initiation in Response to Vernalization
3. Results
3.1. Floral Initiation Responses to Dynamic Changes of Soluble Sugar and Protein Contents of Leaf
3.2. Floral Initiation Responses to Low Temperature for Spring and Semi-Winter Genotypes
3.3. RNA Sequence Quality and Distribution of Samples under Different Treatments
3.4. Modules Constructed by Gene Co-Expression Network Analysis
3.5. Expression of Genes Related to Regulatory Network for Floral Initiation
4. Discussion
5. Conclusions
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Variety | Treatment | Week 1 | Week 2 | Week 3 | Week 4 | Week 6 | Week 8 |
---|---|---|---|---|---|---|---|
1358 | CK | × | × | √ | √ | ||
LT | × | × | √ | √ | |||
Zhongshuang No. 11 | CK | × | × | × | × | × | |
LT | × | × | × | √ | √ |
Sample Code | Bases | Total Reads | Mapped Reads | Q30 (%) | GC (%) |
---|---|---|---|---|---|
CK-H-1 W | 12,346,439,739 | 42,813,771 | 33,688,652 (78.69%) | 94.83 | 48.23 |
CK-H-2 W | 8,380,248,919 | 29,292,740 | 22,657,801 (77.35%) | 95.17 | 48.00 |
CK-H-3 W | 8,336,941,652 | 28,895,492 | 22,374,397 (77.43%) | 94.14 | 48.06 |
CK-H-4 W | 9,640,592,280 | 33,402,546 | 25,926,724 (77.62%) | 94.68 | 48.00 |
CK-Z-1 W | 7,709,963,913 | 26,710,793 | 20,834,744 (78.00%) | 94.94 | 48.38 |
CK-Z-2 W | 8,908,534,540 | 31,058,959 | 24,147,919 (77.75%) | 94.92 | 47.78 |
CK-Z-4 W | 5,647,528,648 | 19,601,439 | 15,278,467 (77.95%) | 95.16 | 47.98 |
CK-Z-6 W | 6,928,211,354 | 24,020,110 | 18,732,732 (77.99%) | 95.14 | 47.88 |
CK-Z-8 W | 5,512,829,371 | 19,128,138 | 14,550,138 (76.07%) | 95.27 | 48.08 |
LT-H-1 W | 5,948,642,517 | 20,705,540 | 16,156,386 (78.03%) | 94.89 | 48.38 |
LT-H-2 W | 6,052,433,689 | 21,111,307 | 15,589,630 (73.84%) | 94.65 | 48.73 |
LT-H-3 W | 7,863,011,312 | 27,799,414 | 15,443,883 (55.55%) | 94.67 | 49.66 |
LT-H-4 W | 5,625,770,861 | 19,546,507 | 15,369,155 (78.63%) | 94.55 | 48.54 |
LT-Z-1 W | 6,504,657,487 | 22,681,919 | 17,851,101 (78.70%) | 95.01 | 48.65 |
LT-Z-2 W | 5,888,026,230 | 20,475,304 | 16,311,490 (79.66%) | 94.63 | 48.31 |
LT-Z-4 W | 6,179,107,275 | 21,605,985 | 16,938,907 (78.40%) | 94.99 | 48.42 |
LT-Z-6 W | 6,065,775,007 | 21,171,760 | 16,631,248 (78.55%) | 95.04 | 48.24 |
LT-Z-8 W | 6,965,309,530 | 24,339,956 | 19,097,804 (78.46%) | 94.97 | 48.13 |
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Luo, T.; Lin, R.; Cheng, T.; Hu, L. Low Temperature Rather Than Nitrogen Application Mainly Modulates the Floral Initiation of Different Ecotypes of Rapeseed (Brassica napus L.). Agronomy 2022, 12, 1624. https://doi.org/10.3390/agronomy12071624
Luo T, Lin R, Cheng T, Hu L. Low Temperature Rather Than Nitrogen Application Mainly Modulates the Floral Initiation of Different Ecotypes of Rapeseed (Brassica napus L.). Agronomy. 2022; 12(7):1624. https://doi.org/10.3390/agronomy12071624
Chicago/Turabian StyleLuo, Tao, Rongmei Lin, Tai Cheng, and Liyong Hu. 2022. "Low Temperature Rather Than Nitrogen Application Mainly Modulates the Floral Initiation of Different Ecotypes of Rapeseed (Brassica napus L.)" Agronomy 12, no. 7: 1624. https://doi.org/10.3390/agronomy12071624