Seaweed Oligosaccharide Synergistic Silicate Improves the Resistance of Rice Plants to Lodging Stress under High Nitrogen Level
Round 1
Reviewer 1 Report
The article “Seaweed Oligosaccharide Synergistic Silicate Improves the 2 Resistance of Rice Plants to Lodging Stress under High 3 Nitrogen Level” described the effect of Si-based biostimulator of rice .
The introduction is available only literature citation about absent effect of Si fertilizers on rice lodging. But it is available also lot literature about positive effect of Si fertilization of rice logging.
Gong, D., Zhang, X., Yao, J., Dai, G., Yu, G., Zhu, Q., ... & Zheng, W. (2021). Synergistic effects of bast fiber seedling film and nano-silicon fertilizer to increase the lodging resistance and yield of rice. Scientific Reports, 11(1), 1-8.
Zhang, S., Yang, Y., Zhai, W., Tong, Z., Shen, T., Li, Y. C., ... & Ding, F. (2019). Controlled‐release nitrogen fertilizer improved lodging resistance and potassium and silicon uptake of direct‐seeded rice. Crop Science, 59(6), 2733-2740.
Liu, X., Huang, Z., Li, Y., Xie, W., Li, W., Tang, X., ... & Mo, Z. (2020). Selenium-silicon (Se-Si) induced modulations in physio-biochemical responses, grain yield, quality, aroma formation and lodging in fragrant rice. Ecotoxicology and Environmental Safety, 196, 110525.
Salman, D., Morteza, S., Dariush, Z., Abbas, G. M., Reza, Y., Ehsan, G. D., & Reza, N. A. (2012). Application of nitrogen and silicon rates on morphological and chemical lodging related characteristics in rice (Oryza sativa L.) at North of Iran. Journal of agricultural science, 4(6), 12.
In discussion is available literature data about positive effect of Si on rice logging.
Please put more information about effect of Si on rice logging in the introduction.
Please also give some suggestion about mechanisms.
Material and results are good.
Author Response
Dear Reviewer,
We are very grateful to your comments for the manuscrip. Based on your comment and request , we have made modification on the original manuscript. Here, Some of your questions were answered below. And we attached revised manuscript in the formats for your approval .A revised manuscript with the correction sections red marked was atached as the supplemental material and for easy check purpose . Should you have any questions , please contact us without hesitate .
Point 1: The article “Seaweed Oligosaccharide Synergistic Silicate Improves the Resistance of Rice Plants to Lodging Stress under High Nitrogen Level” described the effect of Si-based biostimulator of rice. The introduction is available only literature citation about absent effect of Si fertilizers on rice lodging. But it is available also lot literature about positive effect of Si fertilization of rice lodging.
Gong, D.; Zhang, X.; Yao, J.; Dai, G.; Yu, G.; Zhu, Q.; Gao, Q.; Zheng, W. (2021). Synergistic effects of bast fiber seedling film and nano-silicon fertilizer to increase the lodging resistance and yield of rice. Scientific Reports. 11(1), 12788.
Zhang, S.; Yang, Y.; Zhai, W.; Tong, Z.; Shen, T.; Li, Y.C.; Zhang, M.; Sigua, G.; Chen, J.; Ding, F. (2019). Controlled-release nitrogen fertilizer improved lodging resistance and potassium and silicon uptake of direct-seeded rice. Crop Science, 59(6), 2733-2740.
Liu, X., Huang, Z., Li, Y., Xie, W., Li, W., Tang, X., Ashraf, U., Kong, L., Wu, L., Wang, S., … & Mo, Z. (2020). Selenium-silicon (Se-Si) induced modulations in physio-biochemical responses, grain yield, quality, aroma formation and lodging in fragrant rice. Ecotoxicology and Environmental Safety, 196, 110525
Salman, D., Morteza, S., Dariush, Z., Abbas, G.M., Reza, Y., Ehsan, G.D., & Reza, N.A. (2012). Application of nitrogen and silicon rates on morphological and chemical lodging related characteristics in rice (Oryza sativa L.) at North of Iran. Journal of Agricultural Science, 4(6), 12.
Response 1: Thanks for your comments. We have added Si fertilizer literature and rewritten the introduction. Several new Refs, including Gong et al. (2021). Synergistic effects of bast fiber seedling film and nano-silicon fertilizer to increase the lodging resistance and yield of rice in Scientific Reports; Zhang et al. (2019). Controlled-release nitrogen fertilizer improved lodging resistance and potassium and silicon uptake of direct-seeded rice in Crop Science; Liu et al. (2020). Selenium-silicon (Se-Si) induced modulations in physio-biochemical responses, grain yield, quality, aroma formation and lodging in fragrant rice in Ecotoxicology and Environmental Safety; Salman et al. (2012). Application of nitrogen and silicon rates on morphological and chemical lodging related characteristics in rice (Oryza sativa L.) at North of Iran in Journal of Agricultural Science, have been added in the introduction section. Please see line 26 to 55 in the revised manuscript.
Point 2: In discussion is available literature data about positive effect of Si on rice lodging. Please put more information about effect of Si on rice lodging in the introduction.
Response 2: Thanks for your comment. We have added more information on effect of Si on rice lodging in the introduction section. Please see line 39 to 51 in the revised manuscript.
Point 3: Please also give some suggestion about mechanisms.
Response 3: Thanks for your comment. We have added some suggestion about mechanisms. Please see line 274 to 328 in the revised manuscript.
Point 4: Material and results are good.
Response 4: Thanks for your comment and approval.
Author Response File: 
Author Response.docx
Reviewer 2 Report
The manuscript contains quite interesting research results. However, it requires significant improvement, especially the methodology chapter.
Comments:
Citation: Lastname, F.; Lastname, F.; Lastname, F. Title?
Line 62, please include a figure with the location of the test site
Line 62-64, the characteristics of soil and weather conditions, please provide in the tables. Please complete the description of weather conditions with data from many years
Line 67, please specify soil type
Line 67, pH in KCl or H2O?
Line 68, please provide the characteristics of the variety of rice
Line 83, please provide the composition of the fertilizer in the elemental form
Line 110, please provide full details of the manufacturer of the statistical software
Line 160, correct 45 kg/ha
Line 220, (Salman et al., 2012)?
References please remove publications from before 2010 (1, 3, 14, 22, 23, 27, 31, 32)
Line 396, correct seeded rice (Oryza sativaL.). Front. Plant Sci. 2017, 8, 1431.
Author Response
Dear Reviewer,
We are very grateful to your comments for the manuscrip. Based on your comment and request , we have made modification on the original manuscript. Here, Some of your questions were answered below. And we attached revised manuscript in the formats for your approval .A revised manuscript with the correction sections red marked was atached as the supplemental material and for easy check purpose . Should you have any questions , please contact us without hesitate .
Point 1: The manuscript contains quite interesting research results. However, it requires significant improvement, especially the methodology chapter.
Citation: Lastname, F.; Lastname, F.; Lastname, F. Title?
Response 1: Thank you very much for your suggestions on our manuscript. We have revised the whole manuscript, especially the materials and methods. Please see lines 62 to 97 in the revised paper.
Citation (Liu, G.; Ouyang, X.; Li, Z.; Fu, Y.; Shen, H) has been revised.
Point 2: Line 62, please include a figure with the location of the test site
Response 2: Thanks for your comment. We added a figure with the location of the test site. Please see lines 86 to 88 in the revised manuscript.
Point 3: Line 62-64, the characteristics of soil and weather conditions, please provide in the tables. Please complete the description of weather conditions with data from many years.
Response 3: Thanks for your comment. We have included the introduction of soil and weather conditions, please see line 63 to 70 in the revised manuscript.
Point 4: Line 67, please specify soil types
Response 4: Thanks for your comment. We have indicated the soil type, please see line 68 in the revised manuscript.
Point 5: Line 67, pH in KCl or H2O ?
Response 5: Thanks for your comment. We have indicated pH using KCl extraction, please see line 70 in the revised manuscript.
Point 6: Line 68, please provide the characteristics of the variety of rice
Response 6: Thanks for your comment. We have provided the characteristics of rice variety. Please see line 71 to 78 in the revised manuscript.
Point 7: Line 83, please provide the composition of the fertilizer in the elemental form
Response 7: Thanks for your comment. The composition of fertilizer used were shown in line 79 to 85 in the revised manuscript.
Point 8: Line 110, please provide full details of the manufacturer of the statistical software
Response 8: Thanks for your comment. We have indicated the manufacturer of the statistical software, please line 130 in the revised manuscript.
Point 9: References please remove publications from before 2010 (1, 3, 14, 22, 23, 27, 31, 32)
Response: Thanks for your comment. We have removed the publications before 2010. Please see the references section in the revised manuscript.
Point 10: Line 396, correct seeded rice (Oryza sativaL.). Front. Plant Scie. 2017, 8, 1431.
Response 10: Thanks for your comment. We have corrected the mistakes. Please see line 360 to 361 in the revised manuscript.
Author Response File: Author Response.docx
