Restoration of Triticum aestivum Growth under Salt Stress by Phosphate-Solubilizing Bacterium Isolated from Southern Algeria
Abstract
:1. Introduction
2. Material and Methods
2.1. Soil Sampling and Bacterial Isolation
2.2. Halotolerance of the Selected Isolates
2.3. Inorganic Phosphorous Solubilization
2.3.1. On Solid Medium
2.3.2. Quantification of Solubilized Phosphorous in Liquid Medium
2.4. Determination of Phytase Production
2.4.1. On Solid Medium
2.4.2. Quantitative Analysis of Phytase Production
2.5. Molecular Identification of the Selected Isolate
2.6. Wheat Germination and Growth Improvement by the Selected Isolate under Salt Stress
2.6.1. Germination Improvement
2.6.2. Growth Promotion of Wheat Seedlings
2.7. Statistical Analysis
3. Results and Discussion
3.1. Selection of the Isolate N76
3.2. Halotolerance of the Isolate N76
3.3. Molecular Identification of the Selected Isolate
3.4. Phosphorous Solubilization on Solid and Liquid Medium
3.5. Detection and Quantification of Phytase Activity
3.6. Effect of P. azotoformans on Wheat Seed Germination and Wheat Seedling Growth
3.6.1. Effect of P. azotoformans on Wheat Seed Germination
3.6.2. Effect of P. azotoformans on Wheat Seedling Growth
3.7. Quantitative Estimation of Soluble Phosphorous in the Soil
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Strain | NaCl (mM) | 2 Days | 7 Days | ||||
---|---|---|---|---|---|---|---|
SI | Medium’ pH | Soluble—P μg/mL | SI | Medium’ pH | Soluble—P μg/mL | ||
N76 | 0 | 2.87 ± 0.05 | 6.22 ± 0.02 | 136.625 ± 0.01 | 3.5 ± 0.05 | 6.05 ± 0.03 | 142.875 ± 0.23 |
300 | 2.66 ± 0.03 | 6.66 ± 0.02 | 85.375 ± 0.03 | 3 ± 0.05 | 6 ± 0.01 | 141.625 ± 0.1 2 | |
600 | 2.41 ± 0.02 | 6.77 ± 0.04 | 59.225 ± 0.06 | 2.42 ± 0.05 | 6.11 ± 0.01 | 117.875 ± 0.19 | |
900 | 2.2 ± 0.08 | 6.79 ± 0.05 | 54.125 ± 0.02 | 2.33 ± 0.05 | 6.75 ± 0.02 | 59.425 ± 0.08 | |
15 Days | 21 Days | ||||||
NaCl (mM) | SI | Medium’ pH | Soluble—P μg/mL | SI | Medium’ pH | Soluble—P μg/mL | |
0 | 3.87 ± 0.06 | 5.36 ± 0.02 | 242.125 ± 0.25 | 3.5 ± 0.01 | 5.22 ± 0.03 | 242.875 ± 0.34 | |
300 | 4 ± 0.02 | 5.24 ± 0.04 | 255.125 ± 0.13 | 3.5 ± 0.03 | 5.16 ± 0.02 | 255.152 ± 0.11 | |
600 | 3.1 ± 0.03 | 5.74 ± 0.05 | 216.875 ± 0.12 | 3.14 ± 0.02 | 5.58 ± 0.05 | 219.125 ± 0.31 | |
900 | 3.3 ±0.02 | 5.85 ± 0.02 | 201.625 ± 0.32 | 3 ± 0.01 | 5.61 ± 0.02 | 205.375 ± 0.25 |
Strain | NaCl (mM) | 2 Days | 5 Days | 10 Days | |||
---|---|---|---|---|---|---|---|
SI | Soluble—P U/mL | SI | Soluble—P U/mL | SI | Soluble—P U/mL | ||
N76 | 0 | 3.62 ± 0.01 | 0 | 4.33 ± 0.02 | 0.027 ± 0.001 | 5.28 ± 0.04 | 0.168 ± 0.002 |
300 | 3.28 ± 0.03 | 0 | 5.28 ± 0.02 | 0.014 ± 0.004 | 6.5 ± 0.03 | 0.097 ± 0.004 | |
600 | 3.57 ± 0.05 | 0 | 3.9 ± 0.03 | 0.002 ± 0.0001 | 5 ± 0.01 | 0.012 ± 0.001 | |
900 | 3.25 ± 0.02 | 0 | 4.25 ± 0.01 | 0 | 4.85 ± 0.02 | 0.004 ± 0.001 |
0 mM | 100 mM | 200 mM | 300 mM | |
---|---|---|---|---|
Control soil | 10.56 ± 0.33 | 12.6 ± 0.28 | 17.82 ± 0.56 | 17.52 ± 0.62 |
Soil containing N76-inoculated-seeds | 23.3 ± 0.74 | 49.42 ± 0.36 | 19.56 ± 0.45 | 19.5 ± 0.51 |
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Belkebla, N.; Bessai, S.A.; Melo, J.; Caeiro, M.F.; Cruz, C.; Nabti, E.-h. Restoration of Triticum aestivum Growth under Salt Stress by Phosphate-Solubilizing Bacterium Isolated from Southern Algeria. Agronomy 2022, 12, 2050. https://doi.org/10.3390/agronomy12092050
Belkebla N, Bessai SA, Melo J, Caeiro MF, Cruz C, Nabti E-h. Restoration of Triticum aestivum Growth under Salt Stress by Phosphate-Solubilizing Bacterium Isolated from Southern Algeria. Agronomy. 2022; 12(9):2050. https://doi.org/10.3390/agronomy12092050
Chicago/Turabian StyleBelkebla, Nadia, Sylia Ait Bessai, Juliana Melo, Maria Filomena Caeiro, Cristina Cruz, and El-hafid Nabti. 2022. "Restoration of Triticum aestivum Growth under Salt Stress by Phosphate-Solubilizing Bacterium Isolated from Southern Algeria" Agronomy 12, no. 9: 2050. https://doi.org/10.3390/agronomy12092050