Comparative Physiological Analysis of Lignification, Anthocyanin Metabolism and Correlated Gene Expression in Red Toona sinensis Buds during Cold Storage

Round 1

Reviewer 1 Report

The authors provided information on changes in the expression levels of lignin-related and anthocyanins-related genes, as well as changes in the activities of important enzymes and tissue rearrangement during the storage of Toona sinensis, which could provide insight into the molecular mechanism of its senescence.

The following corrections are recommeded.

Line 21: Give the full meaning of PAL, 4CL, CAD and POD; first-time usage.

Line 34: TsCHS and TsANS genes appear not to be found in any database. Can you provide any reference for these genes?

Line 46-48: An incomplete sentence,  please modify; "The buds of red T. sinensisis one of the most popular vegetables in China due to their unique aromatic flavor, more oil, and abundance of proteins, amino acids, vitamins, soluble sugar and minerals"

Line 66: change "coumaroyl-CoA,.Subsequently" to "coumaroyl-CoA. Subsequently, "

Line 75: change "serious" to "series"

Line 79: I suggest you change "As our knowledge," to "To the best of our knowledge,"

Line 97: change "putted" to "put"

Line 102: italicize "T. sinensis"

Line 109 & 116: remove ", respectively"

Line 124-125: remove "amount of plant material and "

Line 126a: replace "should be" with "was".

Line 126b:  change "according to the plant material." to "according to the quantity of the plant material."

Line 132: Did you mean to write "safranin staining solution" instead of "sarranine staining solution"?

Line 142-144: kindly provide link to the reference database or published work containing the 18 (11 and 7) listed genes of interest.

Line 147: change "designed" to "design"

Line 150: insert "contains" between "set" and "three"

Line 160-161: change "T. sinensis buds become dehydration and wilting and the color gradually become dull" to "T. sinensis buds became dehydrated and wilted and the color gradually became dull"

Line 162: replace "change" with "changed"

Line 172: Please, rephrase the following sentence, it is not clear; "The anthocyanins content represented increased firstly after decline during red T. sinensis buds storage."

Line 181: Seperate "Colorchanges"

Line 182-183 & 227-233: I suggest changing "store" to "stored"

Line 251: The statement is not clear.

Line 296:  change "Anthocyanins content red T. sinensis" to ""Anthocyanins content of red T. sinensis"

Line 304: Delete the second "synthesis"

 

Best regards!

Author Response

20 Dec, 2022

Manuscript ID: agronomy-2064623

Title:Comparative Physiological Analysis of Lignification and Anthocyains Metabolism and Correlated Genes Expression in Red Toona sinensis Buds during Cold Storage

Journal name: Agronomy

Manuscript ID: agronomy-2064623

 

Journal: Agronomy

Author:  Qian Zhao, Xiu-Lai Zhong, Xia Cai, Shun-Hua Zhu, Ping-Hong Meng *,

Jian Zhang *, Guo-Fei Tan *

 

Dear Editors and Reviewers,

Thank you and the reviewers very much for revising our manuscript ‘Comparative Physiological Analysis of Lignification and Anthocyains Metabolism and Correlated Genes Expression in Red Toona sinensis Buds during Cold Storage’ (MANUSCRIPT ID: agronomy-2064623). Your effort and time spent on our manuscript are greatly appreciated by all of us. We are delighted to all suggestion and review comments, which you and the reviewers made. Your revisions/suggestions have definitely improved the quality of our manuscript.

This manuscript has been extensively edited according to your and reviewers’ comments. The manuscript has been improved by a professional English language editing service (http://essaystar.com/user/reviewcertificate.html?id=P-202212130846ojz). The manuscript was also critical read by one professional colleague in Canada whose native language is English.Please find the revised manuscript in Agronomy’s manuscript center. The changes were made directly in the text with Trace or RED marked. The responses to the reviewers are highlighted below.

Thank you again for your kind help and excellent suggestions for our manuscript. We hope these revisions will be satisfactory. We are looking forward to hearing from you soon.

 

Yours sincerely

 

Dr. Guo-Fei Tan, [email protected]

Institute of Horticulture, Guizhou Academy of Agricultural Sciences

 

 

 

 

Comments from the editors and reviewers:

 

Reviewer #1:

 

1. The authors provided information on changes in the expression levels of lignin-related and anthocyanins-related genes, as well as changes in the activities of important enzymes and tissue rearrangement during the storage of Toona sinensis, which could provide insight into the molecular mechanism of its senescence.

Response:

-- We thank the reviewer for this observation and suggestion.

 

2. Line 21: Give the full meaning of PAL, 4CL, CAD and POD; first-time usage.

Response:

-- We thank the reviewer for this observation and suggestion.

-- We agree. We appreciate your comments, the full meaning of PAL, 4CL, CAD and POD as follows:

3. Line 34:TsCHS and TsANS genes appear not to be found in any database. Can you provide any reference for these genes?

Response:

-- We thank the reviewer for this observation and suggestion.

-- We agree. We appreciate your comments,We have uploaded the document of the anthocyanin-related genes, including the TsCHS and TsANS genes. The gene sequence of TsCHS and TsANS as follows:

 

 

4. Line 46-48: An incomplete sentence,  please modify; "The buds of red T. sinensisis one of the most popular vegetables in China due to their unique aromatic flavor, more oil, and abundance of proteins, amino acids, vitamins, soluble sugar and minerals"

Response:

-- We thank the reviewer for this observation and suggestion.

-- We agree. We appreciate your comments,we have modified the sentence as follows:

 

5. Line 66: change "coumaroyl-CoA. Subsequently" to "coumaroyl-CoA. Subsequently, "

Response:

-- We thank the reviewer for this observation and suggestion.

-- We agree. We appreciate your comments, we have changed "coumaroyl-CoA. Subsequently" to "coumaroyl-CoA. Subsequently, ". As follows:

 

6. Line 75: change "serious" to "series"

Response:

-- We thank the reviewer for this observation and suggestion.

-- We agree. We appreciate your comments,we have changed "serious" to "series", as follows:

 

7. Line 79: I suggest you change "As our knowledge," to "To the best of our knowledge,".

Response:

-- We thank the reviewer for this observation and suggestion.

-- We agree. We appreciate your comments,we have changed "As our knowledge," to "To the best of our knowledge," ,as follows:

 

8. Line 97: change "putted" to "put"

Response:

-- We thank the reviewer for this observation and suggestion.

-- We agree. We appreciate your comments,we have changed "putted" to "put". as follows:

 

9. Line 102: italicize "T. sinensis"

Response:

-- We thank the reviewer for this observation and suggestion.

-- We agree. We appreciate your comments,we have revised this sentence. As follows:

 

10. Line 109 & 116: remove ", respectively"

Response:

-- We thank the reviewer for this observation and suggestion.

-- We agree. We appreciate your comments,we have removed ", respectively". As follows:

11. Line 124-125: remove "amount of plant material and "

Response:

-- We thank the reviewer for this observation and suggestion.

-- We agree. We appreciate your comments,we have removed "amount of plant material and". As follows:

 

12. Line 126a: replace "should be" with "was".

Response:

-- We thank the reviewer for this observation and suggestion.

-- We agree. We appreciate your comments,we have replace "should be" with "was". As follows:

13. Line 126b:  change "according to the plant material." to "according to the quantity of the plant material."

Response:

-- We thank the reviewer for this observation and suggestion.

-- We agree. We appreciate your comments,we have changed "according to the plant material." to "according to the quantity of the plant material." As follow

14. Line 132: Did you mean to write "safranin staining solution" instead of "sarraninestaining solution"?

Response:

-- We thank the reviewer for this observation and suggestion.

-- We agree. We appreciate your comments,we have changed "sarranine staining solution" to "safranin staining solution" As follow:

 

15. Line 142-144: kindly provide link to the reference database or published work containing the 18 (11 and 7) listed genes of interest.

Response:

-- We thank the reviewer for this observation and suggestion.

-- We agree. We appreciate your comments,we have uploaded the document of the lignin-related and anthocyanin-related genes.The 18 genes sequences as follows:

 

 

 

 

 

 

 

 

16. Line 147: change "designed" to "design"

Response:

-- We thank the reviewer for this observation and suggestion.

-- We agree. We appreciate your comments,we have changed "designed" to "design". As follows:

 

17. Line 150: insert "contains" between "set" and "three"

Response:

-- We thank the reviewer for this observation and suggestion.

-- We agree. We appreciate your comments,we have modified the sentence. As follows:

 

18. Line 160-161: change "T. sinensis buds become dehydration and wilting and the color gradually become dull" to "T. sinensis buds became dehydrated and wilted and the color gradually became dull"

Response:

-- We thank the reviewer for this observation and suggestion.

-- We agree. We appreciate your comments,we have modified the sentence: the leaves of the red T. sinensis buds became dehydrated and wilted,and the color gradually became dull.

 

19. Line 162: replace "change" with "changed"

Response:

-- We thank the reviewer for this observation and suggestion.

-- We agree. We appreciate your comments,we have replaced "change" with "changed". As follows:

 

20. Line 172: Please, rephrase the following sentence, it is not clear; "The anthocyanins content represented increased firstly after decline during red T. sinensisbuds storage."

Response:

-- We thank the reviewer for this observation and suggestion.

-- We agree. We appreciate your comments,we have rephrased the sentence. As follows:

 

21. Line 181: Seperate "Colorchanges"

Response:

-- We thank the reviewer for this observation and suggestion.

-- We agree. We appreciate your comments, we have separated ”Color changes”. As follows:

 

22. Line 182-183 & 227-233: I suggest changing "store" to "stored"

Response:

-- We thank the reviewer for this observation and suggestion.

-- We agree. We appreciate your comments,we have changed “store” to “stored”.As follow:

 

 

23. Line 251: The statement is not clear.

Response:

-- We thank the reviewer for this observation and suggestion.

-- We agree. We appreciate your comments,we have modified the sentence. As follow:

24. Line 296: change "Anthocyanins content red T. sinensis" to "Anthocyanins content of red T. sinensis"

Response:

-- We thank the reviewer for this observation and suggestion.

-- We agree. We appreciate your comments,we have change "Anthocyanins content red T. sinensis" to ""Anthocyanins content of red T. sinensis".As follows:

 

25. Line 304: Delete the second "synthesis"

Response:

-- We thank the reviewer for this observation and suggestion.

-- We agree. We appreciate your comments,we have deleted the second “synthesis”. As follows:

 

In addition, the manuscript has been improved by a professional English language editing service (http://essaystar.com/user/reviewcertificate.html?id=P-202212130846ojz). The manuscript was also critical read by one professional colleague in Canada whose native language is English.

Author Response File: Author Response.docx

Reviewer 2 Report

In this work the anthocyanin and lignin content and the expression of some genes involved in the metabolism of these compounds were evaluated, and some of the evaluated genes related to the observed changes in accumulation of anthocyanins and lignin, during cold storage post-harvesting. Also, some anatomical changes associated to the post-harvesting cold storage are described.

Minor comments to the manuscript:

I suggest some changes in the English writing, as I indicate in the uploaded document.

The results are interesting, and well structured, but I detect some errors in the description, or some need to be clarified in order to be consistent with the graphic data.

My suggestions and comments are mentioned in the article review file.

Comments for author File: Comments.pdf

Author Response

20 Dec, 2022

Manuscript ID: agronomy-2064623

Title:Comparative Physiological Analysis of Lignification and Anthocyains Metabolism and Correlated Genes Expression in Red Toona sinensis Buds during Cold Storage

Journal name: Agronomy

Manuscript ID: agronomy-2064623

 

Journal: Agronomy

Author:  Qian Zhao, Xiu-Lai Zhong, Xia Cai, Shun-Hua Zhu, Ping-Hong Meng *,

Jian Zhang *, Guo-Fei Tan *

 

Dear Editors and Reviewers,

Thank you and the reviewers very much for revising our manuscript ‘Comparative Physiological Analysis of Lignification and Anthocyains Metabolism and Correlated Genes Expression in Red Toona sinensis Buds during Cold Storage’ (MANUSCRIPT ID: agronomy-2064623). Your effort and time spent on our manuscript are greatly appreciated by all of us. We are delighted to all suggestion and review comments, which you and the reviewers made. Your revisions/suggestions have definitely improved the quality of our manuscript.

This manuscript has been extensively edited according to your and reviewers’ comments. The manuscript has been improved by a professional English language editing service (http://essaystar.com/user/reviewcertificate.html?id=P-202212130846ojz). The manuscript was also critical read by one professional colleague in Canada whose native language is English.Please find the revised manuscript in Agronomy’s manuscript center. The changes were made directly in the text with Trace or RED marked. The responses to the reviewers are highlighted below.

Thank you again for your kind help and excellent suggestions for our manuscript. We hope these revisions will be satisfactory. We are looking forward to hearing from you soon.

 

Yours sincerely

 

Dr. Guo-Fei Tan, [email protected]

Institute of Horticulture, Guizhou Academy of Agricultural Sciences

 

 

 

Comments from the editors and reviewers:

 

Reviewer #2:

1. In this work the anthocyanin and lignin content and the expression of some genes involved in the metabolism of these compounds were evaluated, and some of the evaluated genes related to the observed changes in accumulation of anthocyanins and lignin, during cold storage post-harvesting. Also, some anatomical changes associated to the post-harvesting cold storage are described.

Response:

-- We thank the reviewer for this observation and affirmation.

 

2. I suggest some changes in the English writing, as I indicate in the uploaded document.

Response:

-- We thank the reviewer for this observation and affirmation.

-- the manuscript has been improved by a professional English language editing service (http://essaystar.com/user/reviewcertificate.html?id=P-202212130846ojz). The manuscript was also critical read by one professional colleague in Canada whose native language is English.

 

3. The results are interesting, and well structured, but I detect some errors in the description, or some need to be clarified inorder to be consistent with the graphic data.

Response:

-- We thank the reviewer for this observation and affirmation.

-- We agree. We appreciate your comments,we have modified the errors in the document to make the description consistent with the graphical data.

 

4. My suggestions and comments are mentioned in the article review file.

Response:

-- We thank the reviewer for this observation and affirmation.

-- We agree. We appreciate your comments, we have revised these problems as you mentioned in the article review file, and uploaded the revised manuscript.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Author Response File: Author Response.pdf

Reviewer 3 Report

The manuscript entitled "Comparative Physiological Analysis of Lignification and Anthocyains Metabolism and Correlated Genes Expression in Red Toona sinensis Buds during Cold Storage" authored by Zhao et al. is interesting, but is very poorly written. Many statements are unclear and confusing. It is not clear which leaf, the leaflets, and petiole were sampled for the analysis. The expression pattern of the genes studied in leaves and petiole on cold treatment is not at all clear and needs thorough rewriting. I think that the manuscript have the following several drawbacks about the description, data presentations, and some details and cannot be considered for publication. 

General comments

One of the major claims of authors in this manuscript is the recoloration of the red T. sinensis buds while storage. It is not clear from the provided pictures of Figure 1A the decolaration from day 0-3. Even in Fig. 1A-1 (day 0), we can see the green color that is not significantly changed visually in Fig. 1A-IV. It is better to provide a clear picture of decoloration. 

Major comments

Line 99-101: A portion of the samples were frozen in liquid nitrogen ............. constant weight for lignin content determination. As mentioned above, the red and green color parts are clearly visible in Fig 1A-1 (0 d) and 1A-IV (3 d). Always the results obtained would depend on the sample collection. It is better to explain clearly which leaf was selected and the leaflets and petiole were sampled for the analysis.

Line 161-164: The figure numbers need to be precise, including the sub-numbers. 

Line 170: Anthocyanins and lignin contents are important indexes that influence the aging of red T. sinensis buds. This statement may be shifted to the beginning of Line 165.

Line 171-172: The anthocyanins content of the leaves was higher than that of petioles, and the anthocyanins content of the leaves was 5 times that of the petioles. Which day? Kindly specify.

Line 173: The anthocyanins content represented increased firstly after a decline during red T. sinensis buds storage. Need to rewrite, not clear.

Line 237: ........... TsCAD, and TsCCoAOMT decreased. Figure 4E and 4J, it is not. Need to reconsider the writing style and explain the expression of genes. Similarly, for the genes that explain the increased expression. A few of them are not matching with the claim.

Line 242-244: In the petioles of red T. sinensis buds, the expressive of TsPAL showed a trend of first rising and declining, and the expressive levels of TsPAL stored for 3 d were not significant compared with 0 d. The statement is confusing. At 0 d (control), the expression of PAL is at maximum and then declined at 1 d of refrigeration and constantly increased at 2 and 3 d. 

The authors need to carefully examine the expression pattern of the different genes studied in leaf and petiole and explain it properly, especially considering the control (0 d). I think up to line 256, the authors need to rewrite the statements to give a better picture of the expression pattern of the genes.

Line 261: The relative expressive level of leaves and petioles of red T. sinensis buds were 1 at stored for 0 d. Don't know why this statement needs to be here since earlier, also authors explain the expression data as relative levels. 

Line 263: ..........the expressive levels of TsCHS and TsCHI...... From Fig. 5A, I could not see any difference in expression for TsCHS for 1 and 2 d for leaves. The authors claim there is a slight increase in expression, explain.

Line 260-277: Need thorough rewriting of the gene expression for leaves and petiole for better understanding.

Figure 6 needs to be modified if needs to be incorporated into the manuscript. It is not informative and clear how it will give an overall representation of the senescence mechanism.

Author Response

20 Dec, 2022

Manuscript ID: agronomy-2064623

Title:Comparative Physiological Analysis of Lignification and Anthocyains Metabolism and Correlated Genes Expression in Red Toona sinensis Buds during Cold Storage

Journal name: Agronomy

Manuscript ID: agronomy-2064623

 

Journal: Agronomy

Author:  Qian Zhao, Xiu-Lai Zhong, Xia Cai, Shun-Hua Zhu, Ping-Hong Meng *,

Jian Zhang *, Guo-Fei Tan *

 

Dear Editors and Reviewers,

Thank you and the reviewers very much for revising our manuscript ‘Comparative Physiological Analysis of Lignification and Anthocyains Metabolism and Correlated Genes Expression in Red Toona sinensis Buds during Cold Storage’ (MANUSCRIPT ID: agronomy-2064623). Your effort and time spent on our manuscript are greatly appreciated by all of us. We are delighted to all suggestion and review comments, which you and the reviewers made. Your revisions/suggestions have definitely improved the quality of our manuscript.

This manuscript has been extensively edited according to your and reviewers’ comments. The manuscript has been improved by a professional English language editing service (http://essaystar.com/user/reviewcertificate.html?id=P-202212130846ojz). The manuscript was also critical read by one professional colleague in Canada whose native language is English.Please find the revised manuscript in Agronomy’s manuscript center. The changes were made directly in the text with Trace or RED marked. The responses to the reviewers are highlighted below.

Thank you again for your kind help and excellent suggestions for our manuscript. We hope these revisions will be satisfactory. We are looking forward to hearing from you soon.

 

Yours sincerely

 

Dr. Guo-Fei Tan, [email protected]

Institute of Horticulture, Guizhou Academy of Agricultural Sciences

 

 

 

 

Comments from the editors and reviewers:

 

Reviewer #3:

1. The manuscript entitled "Comparative Physiological Analysis of Lignification and Anthocyains Metabolism and Correlated Genes Expression in Red Toona sinensis Buds during Cold Storage" authored by Zhao et al. is interesting, but is very poorly written. Many statements are unclear and confusing. It is not clear which leaf, the leaflets, and petiole were sampled for the analysis. The expression pattern of the genes studied in leaves and petiole on cold treatment is not at all clear and needs thorough rewriting. I think that the manuscript have the following several drawbacks about the description, data presentations, and some details and cannot be considered for publication. 

Response:

-- We thank the reviewer for this observation and affirmation.

-- We agree. We appreciate your comments, we have marked the location of the sample in the figure 1A, and revised the language and description of the manuscript. Other experiments took the whole red T. sinensis buds as experimental material.

 

2. One of the major claims of authors in this manuscript is the recoloration of the red T. sinensis buds while storage. It is not clear from the provided pictures of Figure 1A the decolaration from day 0-3. Even in Fig. 1A-1 (day 0), we can see the green color that is not significantly changed visually in Fig. 1A-IV. It is better to provide a clear picture of decoloration. 

Response:

-- We thank the reviewer for this observation and affirmation.

-- The leaves of red T. sinensis buds did not dislodged at 3 d of storage, indicating that the preservation effect of red T. sinensis buds when refrigerated was better than that at room temperature.

 

3. Line 99-101: A portion of the samples were frozen in liquid nitrogen ............. constant weight for lignin content determination. As mentioned above, the red and green color parts are clearly visible in Fig 1A-1 (0 d) and 1A-IV (3 d). Always the results obtained would depend on the sample collection. It is better to explain clearly which leaf was selected and the leaflets and petiole were sampled for the analysis.

Response:

-- We thank the reviewer for this observation and affirmation.

-- We agree. We appreciate your comments,we have marked the location of the sample inthe figure 1A, and revised the description in Line 99-101. As follows:

4. Line 161-164: The figure numbers need to be precise, including the sub-numbers.

Response:

-- We thank the reviewer for this observation and affirmation.

-- We agree. We appreciate your comments, we have checked and revised the figure numbers and the sub-numbers.

5. Line 170: Anthocyanins and lignin contents are important indexes that influence the aging of red T. sinensis buds. This statement may be shifted to the beginning of Line 165.

Response:

-- We thank the reviewer for this observation and affirmation.

-- We agree. We appreciate your comments,we have changed “Anthocyanins and lignin contents are important indexes that influence the aging of red T. sinensis buds” to the beginning of Line 165. As follows:

 

6. Line 171-172: The anthocyanins content of the leaves was higher than that of petioles, and the anthocyanins content of the leaves was 5 times that of the petioles. Which day? Kindly specify.

Response:

-- We thank the reviewer for this observation and affirmation.

-- We agree. We appreciate your comments,we have revised the sentence. As follows:

 

7. Line 173: The anthocyanins content represented increased firstly after a decline during red T. sinensisbuds storage. Need to rewrite, not clear.

Response:

-- We thank the reviewer for this observation and affirmation.

-- We agree. We appreciate your comments, we have rewritten line 173. As follows:

 

8. Line 237: ........... TsCAD, and TsCCoAOMT decreased. Figure 4E and 4J, it is not. Need to reconsider the writing style and explain the expression of genes. Similarly, for the genes that explain the increased expression. A few of them are not matching with the claim.

Response:

-- We thank the reviewer for this observation and affirmation.

-- We agree. We appreciate your comments,we have checked and rewritten line 237. As follows:

 

9. Line 242-244: In the petioles of red T. sinensis buds, the expressive of TsPAL showed a trend of first rising and declining, and the expressive levels of TsPAL stored for 3 d were not significant compared with 0 d. The statement is confusing. At 0 d (control), the expression of PAL is at maximum and then declined at 1 d of refrigeration and constantly increased at 2 and 3 d.

Response:

-- We thank the reviewer for this observation and affirmation.

-- We agree. We appreciate your comments,we have checked and revised line 242-244. As follows:

 

10. The authors need to carefully examine the expression pattern of the different genes studied in leaf and petiole and explain it properly, especially considering the control (0 d). I think up to line 256, the authors need to rewrite the statements to give a better picture of the expression pattern of the genes.

Response:

-- We thank the reviewer for this observation and affirmation.

-- We agree. We appreciate your comments,we have checked and rewrited the contents of the gene section. As follows:

11. Line 261: The relative expressive level of leaves and petioles of red T. sinensis buds were 1 at stored for 0 d. Don't know why this statement needs to be here since earlier, also authors explain the expression data as relative levels.

Response:

-- We thank the reviewer for this observation and affirmation.

-- We agree. We appreciate your comments,we have checked and revised the contents. As follows:

12. Line 263: ..........the expressive levels of TsCHS andTsCHI...... From Fig. 5A, I could not see any difference in expression forTsCHS for 1 and 2 d for leaves. The authors claim there is a slight increase in expression, explain.

Response:

-- We thank the reviewer for this observation and affirmation.

-- We agree. We appreciate your comments,we have checked and rewrited the contents of the gene section. As follows:

13. Line 260-277: Need thorough rewriting of the gene expression for leaves and petiole for better understanding.

Response:

-- We thank the reviewer for this observation and affirmation.

-- We agree. We appreciate your comments, we have checked and rewrited the contents of the gene section for better understanding. As follows:

 

In addition, the manuscript has been improved by a professional English language editing service (http://essaystar.com/user/reviewcertificate.html?id=P-202212130846ojz). The manuscript was also critical read by one professional colleague in Canada whose native language is English.

 

Author Response File: Author Response.pdf

Reviewer 4 Report

I have read the manuscript, and suggest some minor revisions before to accept the paper for publication in Agronomy.

1.      The authors need to improve the quality of the figures.

2.      Check the abbreviations throughout the manuscript and introduce the abbreviation when the full word appears the first time in the text and then use only the abbreviation (For example, POD etc. in the abstract). And it should be in both abstract as well as in the remaining part of the manuscript. Make a word abbreviated in the article that is repeated at least three times in the text, not all words need to be abbreviated.

3.      The paper needs moderate language revisions  

 

Comments for author File: Comments.docx

Author Response

20 Dec, 2022

Manuscript ID: agronomy-2064623

Title:Comparative Physiological Analysis of Lignification and Anthocyains Metabolism and Correlated Genes Expression in Red Toona sinensis Buds during Cold Storage

Journal name: Agronomy

Manuscript ID: agronomy-2064623

 

Journal: Agronomy

Author:  Qian Zhao, Xiu-Lai Zhong, Xia Cai, Shun-Hua Zhu, Ping-Hong Meng *,

Jian Zhang *, Guo-Fei Tan *

 

Dear Editors and Reviewers,

Thank you and the reviewers very much for revising our manuscript ‘Comparative Physiological Analysis of Lignification and Anthocyains Metabolism and Correlated Genes Expression in Red Toona sinensis Buds during Cold Storage’ (MANUSCRIPT ID: agronomy-2064623). Your effort and time spent on our manuscript are greatly appreciated by all of us. We are delighted to all suggestion and review comments, which you and the reviewers made. Your revisions/suggestions have definitely improved the quality of our manuscript.

This manuscript has been extensively edited according to your and reviewers’ comments. The manuscript has been improved by a professional English language editing service (http://essaystar.com/user/reviewcertificate.html?id=P-202212130846ojz). The manuscript was also critical read by one professional colleague in Canada whose native language is English.Please find the revised manuscript in Agronomy’s manuscript center. The changes were made directly in the text with Trace or RED marked. The responses to the reviewers are highlighted below.

Thank you again for your kind help and excellent suggestions for our manuscript. We hope these revisions will be satisfactory. We are looking forward to hearing from you soon.

 

Yours sincerely

 

Dr. Guo-Fei Tan, [email protected]

Institute of Horticulture, Guizhou Academy of Agricultural Sciences

 

 

 

 

 

Comments from the editors and reviewers:

 

Reviewer #4:

1. I have read the manuscript, and suggest some minor revisions before to accept the paper for publication in Agronomy.

Response:

-- We thank the reviewer for this observation and suggestion.

 

2. Check the abbreviations throughout the manuscript and introduce the abbreviation when the full word appears the first time in the text and then use only the abbreviation (For example, POD etc. in the abstract). And it should be in both abstract as well as in the remaining part of the manuscript. Make a word abbreviated in the article that is repeated at least three times in the text, not all words need to be abbreviated.

Response:

-- We thank the reviewer for this observation and affirmation.

-- We agree. We appreciate your comments,we have checked and revised the abbreviations. As follows:

 

3. The paper needs moderate language revisions.

Response:

-- We thank the reviewer for this observation and affirmation.

-- the manuscript has been improved by a professional English language editing service (http://essaystar.com/user/reviewcertificate.html?id=P-202212130846ojz). The manuscript was also critical read by one professional colleague in Canada whose native language is English.

Author Response File: Author Response.docx

Round 2

Reviewer 3 Report

The manuscript entitled "Comparative Physiological Analysis of Lignification, Anthocyanin Metabolism and Correlated Gene Expression in Red Toona sinensis Buds during Cold Storage" was authored by Zhao et al. improved significantly after thorough corrections and language edits. A few spellchecks and typos need to address, and the corrections are mentioned in the attached file. The manuscript may be now accepted for publication after minor corrections.

Comments for author File: Comments.pdf

Author Response

Please see the uploaded file about comment response!

Author Response File: Author Response.docx