Comparative Transcriptome-Based Analysis of the Regulation of Leaf Senescence in the Upper and Middle Canopy of Different Soybean Cultivars
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials and Growth Conditions
2.2. Determination of Samples, Location and Period of Sampling
2.3. Determination of Chlorophyll and Leaf SPAD
2.4. Determination of Antioxidant Reductase
2.5. Leaf gas Exchange and Chlorophyll Fluorescence Analysis
2.6. RNA-Seq Analysis
2.7. Quantitative Real-Time PCR (qRT-PCR) Analysis
2.8. Data Analysis
3. Results
3.1. Leaf Phenotypes and Physiological Indices of Soybean
3.2. Transcriptome Differences and Enrichment Analysis
3.3. Transcriptional Analysis Reveals Enrichment Pathways Involved in Upper and Middle Leaf Senescence in the Soybean Canopy at Soybean Grain-Filling Stage
3.4. Analysis of Senescence Regulatory Pathways in the Upper and Middle Leaves of Soybean Canopy at Soybean Grain-Filling Stage
3.5. Differentially Expressed Genes in Photosynthesis-Related Pathways Involved in the Regulation of Senescence of Upper and Middle Leaves in the Soybean Canopy at Soybean Grain-Filling Stage
3.6. Transcriptional Analysis Reveals Differentially Expressed Genes Involved in Leaf Senescence in the Middle of the Soybean Canopy at Soybean Grain-Filling Stage
3.7. Validation of DEGs by qRT-PCR Analysis
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Sample Code | Representative Meaning |
---|---|
LD32-T1-UP (S1) | LD32 upper canopy leaves sampled for the first time during the seed filling |
LD32-T1-MID (S2) | LD32 middle canopy leaves sampled for the first time during the seed filling |
LD32-T2-UP (S3) | LD32 upper canopy leaves sampled for the second time during the grain filling |
LD32-T2-MID (S4) | LD32 middle canopy leaves sampled for the second time during the grain filling |
LD32-T3-UP (S5) | LD32 upper canopy leaves sampled for the third time during the grain filling |
LD32-T3-MID (S6) | LD32 middle canopy leaves sampled for the third time during the grain filling |
SND28-T1-UP (S7) | SND28 upper canopy leaves sampled for the first time during the seed filling |
SND28-T1-MID (S8) | SND28 middle canopy leaves sampled for the first time during the seed filling |
SND28-T2-UP (S9) | SND28 upper canopy leaves sampled for the second time during the grain filling |
SND28-T2-MID (S10) | SND28 middle canopy leaves sampled for the second time during the grain filling |
SND28-T3-UP (S11 | SND28 upper canopy leaves sampled for the third time during the grain filling |
SND28-T3-MID (S12) | SND28 middle canopy leaves sampled for the third time during the grain filling |
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Wang, N.; Zhang, Z.; Li, J.; Li, R.; Zhang, X.; Yao, X.; Xie, F. Comparative Transcriptome-Based Analysis of the Regulation of Leaf Senescence in the Upper and Middle Canopy of Different Soybean Cultivars. Agronomy 2024, 14, 1250. https://doi.org/10.3390/agronomy14061250
Wang N, Zhang Z, Li J, Li R, Zhang X, Yao X, Xie F. Comparative Transcriptome-Based Analysis of the Regulation of Leaf Senescence in the Upper and Middle Canopy of Different Soybean Cultivars. Agronomy. 2024; 14(6):1250. https://doi.org/10.3390/agronomy14061250
Chicago/Turabian StyleWang, Nan, Zhenghao Zhang, Jiayi Li, Ruoning Li, Xuejing Zhang, Xingdong Yao, and Futi Xie. 2024. "Comparative Transcriptome-Based Analysis of the Regulation of Leaf Senescence in the Upper and Middle Canopy of Different Soybean Cultivars" Agronomy 14, no. 6: 1250. https://doi.org/10.3390/agronomy14061250