Effects of Foliar Ca and Mg Nutrients on the Respiration of ‘Feizixiao’ Litchi Pulp and Identification of Differential Expression Genes Associated with Respiration
Abstract
:1. Introduction
2. Materials and Methods
2.1. Experimental Setup and Treatment
2.2. Procedures of Sample Collection
2.3. Determination of Total Sugar, Total Acids, and Sugar–Acid Ratio
2.4. Measurement of Total Respiration Rate and Rates of TCA, PPP, and EMP
2.5. Detection of Succinate Dehydrogenase (SDH) and Pyruvate Dehydrogenase Complex (PDC)
2.6. Detection of Glucose Phosphate Isomerase (GPI), Pyruvate Kinase (PK)
2.7. Detection of Malate Dehydrogenase (NAD-MDH) Activity
2.8. Detection of Cytochrome Oxidase (CCO)
2.9. Analysis of Differential Gene Screening and Pathway Selection
2.10. Validation of qRT-PCR
2.11. Data Analysis
3. Results
3.1. Changes in Sugar and Acid Contents in Pulp
3.2. Different Changes in Respiration Rate in Pulp
3.3. Changes in Enzyme Activities in Pulp
3.4. Differential Gene Screening and Role of EMP, PPP, and TCA
3.5. Quantitative RT-PCR
4. Discussion
4.1. Impact of Ca+Mg on Sugar Content and Fruit Quality
4.2. Influence of Ca+Mg on Respiration and Metabolic Pathways
4.3. Effect of Ca+Mg Treatment on Enzymatic Activity and Metabolic Processes
5. Conclusions
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Primer Names | Right Primer Sequences (5′ to 3′) | Left Primer Sequences (5′ to 3′) | Amplification Length |
---|---|---|---|
Cluster-6206.72872 | TGGAAAACGGTGGTGTGGAA | AACAGCTTCTCCACCATCGG | 100–200 bp |
Cluster-6206.110990 | TTGTGGCTGAGTGCACTAGG | TCCACCACTTTCCCAACTGG | 100–200 bp |
Cluster-6206.117009 | TCCCCAACAATGAACCAGGG | CACTCGAATTCAAGCGTCGC | 100–200 bp |
β-Actin | AGTTTGGTTGATGTGGGAGAC | TGGCTGAACCCGAGATGAT | 100–200 bp |
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Sajjad, M.; Tahir, H.; Ma, W.; Shaopu, S.; Farooq, M.A.; Zeeshan Ul Haq, M.; Sajad, S.; Zhou, K. Effects of Foliar Ca and Mg Nutrients on the Respiration of ‘Feizixiao’ Litchi Pulp and Identification of Differential Expression Genes Associated with Respiration. Agronomy 2024, 14, 1347. https://doi.org/10.3390/agronomy14071347
Sajjad M, Tahir H, Ma W, Shaopu S, Farooq MA, Zeeshan Ul Haq M, Sajad S, Zhou K. Effects of Foliar Ca and Mg Nutrients on the Respiration of ‘Feizixiao’ Litchi Pulp and Identification of Differential Expression Genes Associated with Respiration. Agronomy. 2024; 14(7):1347. https://doi.org/10.3390/agronomy14071347
Chicago/Turabian StyleSajjad, Muhammad, Hassam Tahir, Wuqiang Ma, Shi Shaopu, Muhammad Aamir Farooq, Muhammad Zeeshan Ul Haq, Shoukat Sajad, and Kaibing Zhou. 2024. "Effects of Foliar Ca and Mg Nutrients on the Respiration of ‘Feizixiao’ Litchi Pulp and Identification of Differential Expression Genes Associated with Respiration" Agronomy 14, no. 7: 1347. https://doi.org/10.3390/agronomy14071347