Next Article in Journal
The Soil Ecological Stoichiometry Characteristics of the Highest Latitude Areas in the Main Tea-Producing Regions of China
Previous Article in Journal
Effects of Fungicides on Fungal Development, Conidiophore Morphology, and Conidial Releases from Single Strawberry Powdery Mildew Colonies Assessed Using an Electrostatic Technique
 
 
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Article

Identification of miRNAs Interacting with Abscisic Acid to Regulate Fatty Acid Metabolism

by
Zhijun Xu
1,
Zhenxie Yi
1 and
Jing Zhao
2,*
1
College of Agronomy, Hunan Agricultural University, Changsha 410128, China
2
College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China
*
Author to whom correspondence should be addressed.
Agronomy 2024, 14(7), 1358; https://doi.org/10.3390/agronomy14071358
Submission received: 16 May 2024 / Revised: 8 June 2024 / Accepted: 20 June 2024 / Published: 23 June 2024
(This article belongs to the Section Plant-Crop Biology and Biochemistry)

Abstract

Fatty acids are synthesized and stored in seeds during development in Brassica napus. Understanding the molecular mechanism behind fatty acid biosynthesis during seed development is a crucial research objective. In this study, we proved that exogenous application of abscisic acid (ABA) to the siliques can efficiently improve unsaturated fatty acid content in rapeseeds. Then we identified a total of 97 novel microRNAs (miRNAs) and 211 known miRNAs in the seeds of B. napus by high-throughput sequencing. Among them, a total of 23 differentially expressed miRNAs were observed between siliques treated with ABA and the control group. These 23 miRNAs regulated target genes that were involved in lipid metabolism through the integration of gene ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) annotations. Moreover, we validated selected members from these miRNAs and their predicted targets through quantitative RT-PCR. Among these, miR172a, miR395a, and novel13 were significantly down-regulated after ABA treatment, while novel3 was significantly up-regulated. Notably, the predicted target genes of miR172a and miR395a, namely, KAS I and DGAT, play crucial roles in fatty acid synthesis and exhibit up-regulated expression in response to ABA. Our findings suggest that a variety of miRNAs interact with ABA to regulate fatty acid biosynthesis, highlighting the important roles played by miRNAs in the process of fatty acid biosynthesis during seed development.
Keywords: fatty acids; abscisic acid; microRNA; seed development fatty acids; abscisic acid; microRNA; seed development

Share and Cite

MDPI and ACS Style

Xu, Z.; Yi, Z.; Zhao, J. Identification of miRNAs Interacting with Abscisic Acid to Regulate Fatty Acid Metabolism. Agronomy 2024, 14, 1358. https://doi.org/10.3390/agronomy14071358

AMA Style

Xu Z, Yi Z, Zhao J. Identification of miRNAs Interacting with Abscisic Acid to Regulate Fatty Acid Metabolism. Agronomy. 2024; 14(7):1358. https://doi.org/10.3390/agronomy14071358

Chicago/Turabian Style

Xu, Zhijun, Zhenxie Yi, and Jing Zhao. 2024. "Identification of miRNAs Interacting with Abscisic Acid to Regulate Fatty Acid Metabolism" Agronomy 14, no. 7: 1358. https://doi.org/10.3390/agronomy14071358

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop