Self-Incompatibility and Pollination Efficiency in Coffea canephora Using Fluorescence Microscopy
Abstract
:1. Introduction
2. Material and Methods
2.1. Tester Plants and Breeding Population
2.2. In Vitro Pollination
2.3. Fluorescence Microscopy
2.4. Allelic and Genotypic Frequencies
2.5. Pollination Efficiency
3. Results and Discussion
4. Conclusions
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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n | Genotype | Origin | n | Genotype | Origin |
---|---|---|---|---|---|
1 | AR106 | Aldinei Raasch 7 | 24 | LB10 | Laerte Braun 5 |
2 | AS1 | Ademar Schmidt 2 | 25 | LB12 | Laerte Braun 5 |
3 | AS10 | Ademar Schmidt 2 | 26 | LB15 | Laerte Braun 5 |
4 | AS12 | Ademar Schmidt 2 | 27 | LB160 | Laerte Braun 5 |
5 | AS2 | Ademar Schmidt 2 | 28 | LB20 | Laerte Braun 5 |
6 | AS5 | Ademar Schmidt 2 | 29 | LB33 | Laerte Braun 5 |
7 | AS7 | Ademar Schmidt 2 | 30 | LB68 | Laerte Braun 5 |
8 | BAG24 | Embrapa 1 | 31 | LB80 | Laerte Braun 5 |
9 | BAG27 | Embrapa 1 | 32 | LB88 | Laerte Braun 5 |
10 | BAG28 | Embrapa 1 | 33 | N13 | Nivaldo Ferreira 6 |
11 | BG180 | Adilson Berger 3 | 34 | N16 | Nivaldo Ferreira 6 |
12 | CA1 | Carlos Alves Silva 4 | 35 | N32 | Nivaldo Ferreira 6 |
13 | GB1 | Gilberto Boon 2 | 36 | N7 | Nivaldo Ferreira 6 |
14 | GB4 | Gilberto Boon 2 | 37 | N8[G8] | Nivaldo Ferreira 6 |
15 | GB7 | Gilberto Boon 2 | 38 | P50 | Valdecir Piske 2 |
16 | GJ2 | Geraldo Jacomini 5 | 39 | R152 | Ronaldo G Oliveira 2 |
17 | GJ20 | Geraldo Jacomini 5 | 40 | R22 | Ronaldo Vitoriano 2 |
18 | GJ25 | Geraldo Jacomini 5 | 41 | SK244 | Sergio Kalk 6 |
19 | GJ3 | Geraldo Jacomini 5 | 42 | SK41 | Sergio Kalk 6 |
20 | GJ30 | Geraldo Jacomini 5 | 43 | SK80 | Sergio Kalk 6 |
21 | GJ5 | Geraldo Jacomini 5 | 44 | VP156 | Valdecir Piske 2 |
22 | GJ8 | Geraldo Jacomini 5 | 45 | WP6 | Wanderley Peter 6 |
23 | L1 | Alcides Rosa 3 |
n | Genotype | C | NC | FN | Group | p(FN) | n | Genotype | C | NC | FN | Group | p(FN) |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 | AR106 | 5 | 1 | 2 | III | 0.05 | 24 | LB10 | 5 | 2 | 4 | III | <0.01 |
2 | AS1 | 5 | 2 | 1 | VI | <0.01 | 25 | LB12 | 5 | 1 | 3 | II | 0.05 |
3 | AS10 | 5 | 1 | 4 | VI | 0.05 | 26 | LB15 | 5 | 1 | 0 | III | 0.05 |
4 | AS12 | 5 | 1 | 0 | VI | 0.05 | 27 | LB160 | 5 | 1 | 4 | I | 0.05 |
5 | AS2 | 5 | 1 | 0 | III | 0.05 | 28 | LB20 | 5 | 1 | 2 | II | 0.05 |
6 | AS5 | 5 | 1 | 1 | II | 0.05 | 29 | LB33 | 5 | 1 | 1 | III | 0.05 |
7 | AS7 | 5 | 1 | 1 | II | 0.05 | 30 | LB68 | 5 | 1 | 2 | II | 0.05 |
8 | BAG24 | 5 | 1 | 2 | II | 0.05 | 31 | LB80 | 5 | 2 | 0 | II | <0.01 |
9 | BAG27 | 5 | 1 | 1 | I | 0.05 | 32 | LB88 | 5 | 1 | 0 | III | 0.05 |
10 | BAG28 | 5 | 1 | 0 | III | 0.05 | 33 | N13 | 5 | 1 | 1 | III | 0.05 |
11 | BG180 | 5 | 1 | 0 | VI | 0.05 | 34 | N16 | 5 | 1 | 0 | VI | 0.05 |
12 | CA1 | 5 | 3 | 1 | VI | <0.01 | 35 | N32 | 5 | 3 | 0 | I | <0.01 |
13 | GB1 | 5 | 1 | 1 | I | 0.05 | 36 | N7 | 5 | 2 | 1 | I | <0.01 |
14 | GB4 | 5 | 1 | 2 | II | 0.05 | 37 | N8[G8] | 5 | 1 | 0 | II | 0.05 |
15 | GB7 | 5 | 1 | 3 | I | 0.05 | 38 | P50 | 5 | 1 | 1 | II | 0.05 |
16 | GJ2 | 5 | 2 | 1 | I | <0.01 | 39 | R152 | 5 | 1 | 1 | III | 0.05 |
17 | GJ20 | 5 | 1 | 0 | III | 0.05 | 40 | R22 | 5 | 2 | 0 | IV | <0.01 |
18 | GJ25 | 5 | 1 | 0 | II | 0.05 | 41 | SK244 | 5 | 1 | 0 | I | 0.05 |
19 | GJ3 | 5 | 1 | 1 | V | 0.05 | 42 | SK41 | 5 | 1 | 2 | II | 0.05 |
20 | GJ30 | 5 | 2 | 0 | I | <0.01 | 43 | SK80 | 5 | 1 | 3 | II | 0.05 |
21 | GJ5 | 5 | 1 | 1 | II | 0.05 | 44 | VP156 | 5 | 1 | 1 | VI | 0.05 |
22 | GJ8 | 5 | 1 | 2 | I | 0.05 | 45 | WP6 | 5 | 2 | 2 | I | <0.01 |
23 | L1 | 5 | 1 | 1 | III | 0.05 |
n | I | II | III | IV | V | VI |
---|---|---|---|---|---|---|
S1S2 | S1S3 | S2S3 | S(2,3)S4 | S(2,3)S4 | S1S4 | |
1 | BAG27 | BAG24 | AR106 | R22 | GJ3 | AS1 |
2 | GB1 | AS5 | AS2 | AS10 | ||
3 | GB7 | AS7 | BAG28 | AS12 | ||
4 | GJ2 | GB4 | GJ20 | BG180 | ||
5 | GJ8 | GJ5 | L1 | CA1 | ||
6 | GJ30 | GJ25 | LB10 | N16 | ||
7 | LB160 | LB12 | LB15 | VP156 | ||
8 | N7 | LB20 | LB33 | |||
9 | N32 | LB68 | LB88 | |||
10 | SK244 | LB80 | N13 | |||
11 | WP6 | N8[G8] | R152 | |||
12 | P50 | |||||
13 | SK41 | |||||
14 | SK80 | |||||
Total | 11 | 14 | 11 | 1 | 1 | 7 |
% | 24.44 | 31.11 | 24.44 | 2.22 | 2.22 | 15.57 |
Alleles | Allelic Frequency | Groups | Genotypes | Genotypic Frequency | Expected Value |
---|---|---|---|---|---|
S1 | 0.36 | I | P(S1S2) | 0.25 | 11.44 |
S2 | 0.26 | II | P(S1S3) | 0.29 | 12.93 |
S3 | 0.29 | III | P(S2S3) | 0.21 | 9.30 |
S4 | 0.10 | IV | P(S2S4) | 0.07 | 3.22 |
V | P(S3S4) | 0.08 | 3.64 | ||
VI | P(S1S4) | 0.10 | 4.48 | ||
Groups | O | E | O-E | (O-E)2 | (O-E)2/E |
I | 11 | 11.44 | −0.44 | 0.19 | 0.02 |
II | 14 | 12.93 | 1.07 | 1.14 | 0.09 |
III | 11 | 9.30 | 1.70 | 2.91 | 0.31 |
IV | 1 | 3.22 | −2.22 | 4.92 | 1.53 |
V | 1 | 3.64 | −2.64 | 6.96 | 1.91 |
VI | 7 | 4.48 | 2.52 | 6.37 | 1.42 |
5.28 NS |
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Silva, A.N.R.; Rocha, R.B.; Teixeira, A.L.; Espindula, M.C.; Partelli, F.L.; Caixeta, E.T. Self-Incompatibility and Pollination Efficiency in Coffea canephora Using Fluorescence Microscopy. Agronomy 2024, 14, 1564. https://doi.org/10.3390/agronomy14071564
Silva ANR, Rocha RB, Teixeira AL, Espindula MC, Partelli FL, Caixeta ET. Self-Incompatibility and Pollination Efficiency in Coffea canephora Using Fluorescence Microscopy. Agronomy. 2024; 14(7):1564. https://doi.org/10.3390/agronomy14071564
Chicago/Turabian StyleSilva, Adriele Nunes Rodrigues, Rodrigo Barros Rocha, Alexsandro Lara Teixeira, Marcelo Curitiba Espindula, Fábio Luiz Partelli, and Eveline Teixeira Caixeta. 2024. "Self-Incompatibility and Pollination Efficiency in Coffea canephora Using Fluorescence Microscopy" Agronomy 14, no. 7: 1564. https://doi.org/10.3390/agronomy14071564