Estimation of Leaf Water Content of a Fruit Tree by In Situ Vis-NIR Spectroscopy Using Multiple Machine Learning Methods in Southern Xinjiang, China

Water scarcity is one of the most significant environmental factors that inhibits photosynthesis and decreases the growth and productivity of plants. Using the deep learning convolutional neural network (CNN) model, this study evaluates the ability of spectroscopy to estimate leaf water content (LWC) in fruit trees. During midday, spectral data were acquired from leaf samples obtained from three distinct varieties of fruit trees, encompassing the spectral range spanning from 350 to 2500 nm. Then, for spectral preprocessing, the fractional order derivative (FOD) and continuous wavelet transform (CWT) algorithms were used to reduce the effects of scattering and noise on the collected spectra. Finally, the CNN model was developed to predict LWC in different fruit trees. The results showed that: (1) The spectra treated with CWT and FOD could improve the spectrum expression ability by improving the correlation between spectra and LWC. The correlation level of FOD treatment was higher than that of CWT treatment. (2) The CNN model was developed using FOD _1.2, and CWT ₃ performed better than other traditional machine learning methods, such as RFR, SVR, and PLSR. (3) Further validation using additional samples demonstrated that the CNN model had good stability and quantitative prediction capability for the LWC of fruit trees (R² > 0.95, root mean square error (RMSE) < 1.773%, and relative percentage difference (RPD) > 4.26). The results may provide an effective way to predict fruit LWC using a CNN-based model.