The Promotion of Dark Septate Endophytes on the Performance and Active Ingredients Accumulation of Astragalus mongholicus under Cadmium Stress
Abstract
:1. Introduction
2. Materials and Methods
2.1. Biological Materials and Culture Substrate
2.2. Inoculation Assay
2.3. DSE Colonization of Plant Roots
2.4. Plant Growth Parameters and Cd Contents
2.5. IAA and Active Ingredient Content
2.6. Soil Physicochemical Properties
2.7. Statistical Analyses
3. Results
3.1. Quantification of DSE Colonization
3.2. Plant Growth Parameters
3.3. IAA, Active Ingredients and Cd Content
3.4. Soil Physicochemical Properties
3.5. Factors Affecting the Growth of the Host Plant
4. Discussion
4.1. Effect of DSE Strains on the Growth of the Host Plant
4.2. Effect of DSE Inoculation on Active Ingredients
4.3. Effect of DSE Strains on Cd Absorption in Medicinal Plants
4.4. Effect of DSE Strains on Soil Factors
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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DSE | Cd | DSE × Cd | ||||
---|---|---|---|---|---|---|
F | ηp2 | F | ηp2 | F | ηp2 | |
Height | 60.34 *** | 0.85 | 31.21 *** | 0.75 | 6.44 *** | 0.64 |
Leaf number | 151.10 *** | 0.93 | 35.11 *** | 0.77 | 21.15 *** | 0.86 |
Branch | 61.46 *** | 0.85 | 13.46 *** | 0.56 | 3.05 *** | 0.46 |
SPAD | 56.29 *** | 0.84 | 48.74 *** | 0.82 | 22.85 *** | 0.87 |
Shoot biomass | 126.73 *** | 0.92 | 143.78 *** | 0.93 | 17.10 *** | 0.83 |
Root biomass | 93.06 *** | 0.90 | 144.15 *** | 0.93 | 22.38 *** | 0.86 |
Total biomass | 146.31 *** | 0.93 | 221.79 *** | 0.95 | 15.45 *** | 0.81 |
Root–shoot ratio | 45.73 *** | 0.81 | 17.03 *** | 0.61 | 20.54 *** | 0.85 |
IAA | 56.32 *** | 0.84 | 3.97 * | 0.27 | 13.45 *** | 0.79 |
Root length | 270.15 *** | 0.96 | 91.23 *** | 0.90 | 85.37 *** | 0.96 |
Root volume | 80.27 *** | 0.88 | 83.87 *** | 0.89 | 39.49 *** | 0.92 |
Calycosin-7-O-glucoside | 304.85 *** | 0.97 | 139.96 *** | 0.93 | 19.96 *** | 0.85 |
Formononetin | 614.60 *** | 0.98 | 955.70 *** | 0.99 | 156.82 *** | 0.98 |
Shoot Cd | 277.98 *** | 0.96 | 1037.17 *** | 0.99 | 28.91 *** | 0.89 |
Root Cd | 271.20 *** | 0.96 | 1782.58 *** | 0.99 | 51.45 *** | 0.94 |
Translocation factor | 282.29 *** | 0.96 | 17.59 *** | 0.62 | 19.25 *** | 0.84 |
SOC | 205.64 *** | 0.95 | 203.76 *** | 0.95 | 13.72 *** | 0.79 |
SAN | 142.78 *** | 0.93 | 37.64 *** | 0.78 | 11.95 *** | 0.77 |
SAP | 147.34 *** | 0.93 | 1185.99 *** | 0.99 | 83.49 *** | 0.96 |
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Li, M.; Han, L.; He, C.; Li, X.; He, X. The Promotion of Dark Septate Endophytes on the Performance and Active Ingredients Accumulation of Astragalus mongholicus under Cadmium Stress. Agronomy 2024, 14, 1801. https://doi.org/10.3390/agronomy14081801
Li M, Han L, He C, Li X, He X. The Promotion of Dark Septate Endophytes on the Performance and Active Ingredients Accumulation of Astragalus mongholicus under Cadmium Stress. Agronomy. 2024; 14(8):1801. https://doi.org/10.3390/agronomy14081801
Chicago/Turabian StyleLi, Min, Li Han, Chao He, Xia Li, and Xueli He. 2024. "The Promotion of Dark Septate Endophytes on the Performance and Active Ingredients Accumulation of Astragalus mongholicus under Cadmium Stress" Agronomy 14, no. 8: 1801. https://doi.org/10.3390/agronomy14081801