Evaluation of Somaclonal and Ethyl Methane Sulfonate-Induced Genetic Variation of Mexican Oregano (Lippia graveolens H.B.K.)
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Material
Conditions for In Vitro Germination of Mexican Oregano
2.2. Micropropagation of Mexican Oregano
2.3. Chemical Mutagenesis in Axillary Buds of L. graveolens
2.4. Analysis of the Genetic Variability Using RAMP Markers
2.5. Data Analysis
3. Results
3.1. In Vitro Propagation
3.2. Mutagenesis
3.3. RAMP Analysis
4. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Primer RAPD | Primer ISSR | Combination |
---|---|---|
OPB-07 | ARS-01 | 1 |
OPB-08 | ARS-01 | 2 |
OPB-12 | ARS-01 | 3 |
OPB-07 | ARS-02 | 4 |
OPB-08 | ARS-02 | 5 |
OPB-12 | ARS-02 | 6 |
OPB-07 | ARS-08 | 7 |
OPB-08 | ARS-08 | 8 |
OPB-12 | ARS-08 | 9 |
EMS Concentration (%) | Exposure Time (h) | No. of Shoots per Explant * | Length of the Shoots (cm) * |
---|---|---|---|
0.1 | 1 | 2.9 ± 0.3a | 2.1 ± 0.2x |
0.1 | 2 | 2.0 ± 0.4a | 2.0 ± 0.1x |
0.1 | 3 | 1.2 ± 0.1b | 0.8 ± 0.1y |
0.3 | 1 | 1.5 ± 0.4b | 1.9 ± 0.4x |
0.3 | 2 | 1.0 ± 0.0b | 1.6 ± 0.3x |
0.3 | 3 | 0.0 ± 0.0c | 0.0 ± 0.0z |
0.5 | 1 | 1.3 ± 0.1b | 1.7 ± 0.1x |
0.5 | 2 | 0.2 ± 0.1c | 0.9 ± 0.2y |
0.5 | 3 | 0.0 ± 0.0c | 0.0 ± 0.0z |
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Muñoz-Miranda, L.A.; Rodríguez-Sahagún, A.; Acevedo Hernández, G.J.; Cruz-Martínez, V.O.; Torres-Morán, M.I.; Lépiz-Ildefonso, R.; Aarland, R.C.; Castellanos-Hernández, O.A. Evaluation of Somaclonal and Ethyl Methane Sulfonate-Induced Genetic Variation of Mexican Oregano (Lippia graveolens H.B.K.). Agronomy 2019, 9, 166. https://doi.org/10.3390/agronomy9040166
Muñoz-Miranda LA, Rodríguez-Sahagún A, Acevedo Hernández GJ, Cruz-Martínez VO, Torres-Morán MI, Lépiz-Ildefonso R, Aarland RC, Castellanos-Hernández OA. Evaluation of Somaclonal and Ethyl Methane Sulfonate-Induced Genetic Variation of Mexican Oregano (Lippia graveolens H.B.K.). Agronomy. 2019; 9(4):166. https://doi.org/10.3390/agronomy9040166
Chicago/Turabian StyleMuñoz-Miranda, Luis A., Araceli Rodríguez-Sahagún, Gustavo J. Acevedo Hernández, Victor O. Cruz-Martínez, Martha I. Torres-Morán, Rogelio Lépiz-Ildefonso, Rayn C. Aarland, and Osvaldo A. Castellanos-Hernández. 2019. "Evaluation of Somaclonal and Ethyl Methane Sulfonate-Induced Genetic Variation of Mexican Oregano (Lippia graveolens H.B.K.)" Agronomy 9, no. 4: 166. https://doi.org/10.3390/agronomy9040166