Functions of the Thyroid-Stimulating Hormone on Key Developmental Features Revealed in a Series of Zebrafish Dyshormonogenesis Models
Abstract
:1. Introduction
2. Materials and Methods
2.1. Zebrafish Maintenance and Treatment
2.2. Establishment of Mutant Lines
2.3. RNA Extraction and qPCR
2.4. Whole-Mount Immunofluorescence
2.5. Protein Extraction and Western Blot Analysis
2.6. Analyses of Growth and Developmental Timing
2.7. Thyroid Hormone Quantification
2.8. Behavioral Assays
2.9. Micro-CT Scan
2.10. Statistical Analysis
3. Results
3.1. Generation of Tshba Mutant Zebrafish
3.2. Growth Performance of the Tshba Mutant Zebrafish
3.3. Post-Embryonic Development of Tshba Mutant Zebrafish
3.4. Rescue Effects of the Supplemental Exogenous T3 Treatments on Tshba Mutants
3.5. Reproductive Defects Observed in Tshba Mutant Zebrafish
3.6. Generation of tg Mutant Zebrafish
3.7. Generation of slc16a2 Mutant Zebrafish
3.8. General Phenotype Observed in tg and slc16a2 Mutant Zebrafish
3.9. Divergent Effects of Reproduction in Various Zebrafish Hypothyroidism Models
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Song, J.; Lu, Y.; Cheng, X.; Shi, C.; Lou, Q.; Jin, X.; He, J.; Zhai, G.; Yin, Z. Functions of the Thyroid-Stimulating Hormone on Key Developmental Features Revealed in a Series of Zebrafish Dyshormonogenesis Models. Cells 2021, 10, 1984. https://doi.org/10.3390/cells10081984
Song J, Lu Y, Cheng X, Shi C, Lou Q, Jin X, He J, Zhai G, Yin Z. Functions of the Thyroid-Stimulating Hormone on Key Developmental Features Revealed in a Series of Zebrafish Dyshormonogenesis Models. Cells. 2021; 10(8):1984. https://doi.org/10.3390/cells10081984
Chicago/Turabian StyleSong, Jia, Yao Lu, Xiaoxia Cheng, Chuang Shi, Qiyong Lou, Xia Jin, Jiangyan He, Gang Zhai, and Zhan Yin. 2021. "Functions of the Thyroid-Stimulating Hormone on Key Developmental Features Revealed in a Series of Zebrafish Dyshormonogenesis Models" Cells 10, no. 8: 1984. https://doi.org/10.3390/cells10081984