Integrated Analysis of Transcriptome, microRNAs, and Chromatin Accessibility Revealed Potential Early B-Cell Factor1-Regulated Transcriptional Networks during the Early Development of Fetal Brown Adipose Tissues in Rabbits
Abstract
:1. Introduction
2. Materials and Methods
2.1. Ethics Approval
2.2. Experimental Animals and Histological Observation
2.3. Library Preparation, Sequencing, and Analysis of RNA-Seq
2.4. Library Preparation, Sequencing, and Analysis of miRNA-Seq
2.5. Library Preparation, Sequencing, and Analysis of ATAC-Seq
2.6. Functional Annotation and Pathway Enrichment
2.7. Quantitative Real-Time PCR (RT-qPCR)
2.8. Statistical Analysis
3. Results
3.1. Changes of Histological Characterization and Genome-Wide Gene Expression during the Early FBATs Development in Rabbits
3.2. Identification of miRNAs during Early FBAT Development
3.3. Chromatin Accessibility Changes during the Early FBAT Development in Rabbits
3.4. Proximal Regulation of Chromatin Accessibility and the miRNA Regulation during the Early Development of FBAT
3.5. Genome-Wide Footprinting Analysis Revealed EBF1 Is Important for the Early Development of FBAT
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Du, K.; Shi, Y.; Bai, X.; Chen, L.; Sun, W.; Chen, S.; Wang, J.; Jia, X.; Lai, S. Integrated Analysis of Transcriptome, microRNAs, and Chromatin Accessibility Revealed Potential Early B-Cell Factor1-Regulated Transcriptional Networks during the Early Development of Fetal Brown Adipose Tissues in Rabbits. Cells 2022, 11, 2675. https://doi.org/10.3390/cells11172675
Du K, Shi Y, Bai X, Chen L, Sun W, Chen S, Wang J, Jia X, Lai S. Integrated Analysis of Transcriptome, microRNAs, and Chromatin Accessibility Revealed Potential Early B-Cell Factor1-Regulated Transcriptional Networks during the Early Development of Fetal Brown Adipose Tissues in Rabbits. Cells. 2022; 11(17):2675. https://doi.org/10.3390/cells11172675
Chicago/Turabian StyleDu, Kun, Yu Shi, Xue Bai, Li Chen, Wenqiang Sun, Shiyi Chen, Jie Wang, Xianbo Jia, and Songjia Lai. 2022. "Integrated Analysis of Transcriptome, microRNAs, and Chromatin Accessibility Revealed Potential Early B-Cell Factor1-Regulated Transcriptional Networks during the Early Development of Fetal Brown Adipose Tissues in Rabbits" Cells 11, no. 17: 2675. https://doi.org/10.3390/cells11172675