Human Adipose Mesenchymal Stromal/Stem Cells Improve Fat Transplantation Performance
Abstract
:1. Introduction
2. Materials and Methods
2.1. Isolation and Expansion of Human AD-MSCs
2.2. Fluorescence-Activated Cell Sorting Analyses (FACS)
2.3. Differentiation Assays
2.4. Prostaglandin E2 (PGE2) Evaluation in AD-MSCs
2.5. Retroviral Transduction of Human AD-MSCs to Express GFP
2.6. Microbiology Test
2.7. Human AD-MSC-Assisted Autologous Fat Transplantation in a Xenogeneic Model
2.8. Hematology and Biochemistry
2.9. Tissue Handling and Histology
2.10. Statistical Analysis
3. Results
3.1. AD-MSCs Could Be Isolated and Genetically Modified from Small Amounts of Adipose Tissue
3.2. Xenotransplantation Was Well Tolerated by NOD/SCID Mice
3.3. AD-MSC-GFP Differentiated into Adipocytes within 60 Days after Transplantation
3.4. Proangiogenic and Pro-Inflammatory Characterization of AFT-AD-MSC-GFP
3.5. Wild-Type AD-MSCs Preserved Their Proangiogenic and Anti-Inflammatory Properties
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Piccinno, M.S.; Petrachi, T.; Pignatti, M.; Murgia, A.; Grisendi, G.; Candini, O.; Resca, E.; Bergamini, V.; Ganzerli, F.; Portone, A.; et al. Human Adipose Mesenchymal Stromal/Stem Cells Improve Fat Transplantation Performance. Cells 2022, 11, 2799. https://doi.org/10.3390/cells11182799
Piccinno MS, Petrachi T, Pignatti M, Murgia A, Grisendi G, Candini O, Resca E, Bergamini V, Ganzerli F, Portone A, et al. Human Adipose Mesenchymal Stromal/Stem Cells Improve Fat Transplantation Performance. Cells. 2022; 11(18):2799. https://doi.org/10.3390/cells11182799
Chicago/Turabian StylePiccinno, Maria Serena, Tiziana Petrachi, Marco Pignatti, Alba Murgia, Giulia Grisendi, Olivia Candini, Elisa Resca, Valentina Bergamini, Francesco Ganzerli, Alberto Portone, and et al. 2022. "Human Adipose Mesenchymal Stromal/Stem Cells Improve Fat Transplantation Performance" Cells 11, no. 18: 2799. https://doi.org/10.3390/cells11182799