1. Introduction
Peripheral nerve injury triggers the spontaneous reprogramming of Schwann cells to a reparative phenotype that scavenges myelin debris, supports axonal survival, and directs axonal regrowth across lesion gaps small enough for spontaneous recovery from injury [
1]. Spontaneous repair becomes limited [
2] when disruptive damage to the nerve exceeds the critical gap. Such cases can be remedied by bridging the stumps with nerve autografts or Schwann-cell-seeded axon guidance conduits that support the regeneration of axon–Schwann cell units across the severed tissue not only in the peripheral nervous system (PNS) [
3,
4,
5], but also in the central nervous system (CNS) [
6,
7].
Given the lack of accessible Schwann cells from native tissues and the fate instability of Schwann cells derived in vitro, the clinical use of Schwann cell transplantation for tissue repair and remyelination has been limited. Primary Schwann cells that can be harvested from native tissues are limited by donor site morbidity, whereas Schwann-cell-like cells (SCLCs) derived from non-neural tissues [
8,
9] often display phenotypic instability and spontaneous dedifferentiation into myofibroblasts [
10,
11,
12] unless steps are taken to promote commitment.
To this end, we derived a protocol whereby Schwann cells were derived from rats (pilot studies) and, subsequently, human bone marrow stromal cells (hBMSCs) [
11,
13,
14], without the use of transforming viral vectors. A culture of hBMSCs in non-adherent conditions facilitated the expansion of neural stem/progenitor cells that were positive for nestin and glial fibrillary acid protein (GFAP) [
15]. These cells were directed to differentiate into SCLCs with supplementation of glia-inducing factors (GIFs) in the culture medium [
11,
13,
14]. Via the co-culture of SCLCs and embryonic rat dorsal root ganglia (DRG) neurons, contact-mediated Notch and ErbB signaling was found to direct the commitment of SCLCs to the Schwann cell fate [
12]. However, the use of cross-species cells to generate SCLCs invites the risk of immune hypersensitivity and deters the use of such SCLC derivatives for transplantation therapy.
To replace rat DRG neurons with a human counterpart for inducing the lineage commitment of SCLCs, we made use of a rapid single-step protocol to generate sensory neurons (hiSN) from human induced pluripotent stem cells (hiPSCs) [
14]. In the current work, the co-culture of hSCLCs with hiSNs was found to mediate fate commitment of hBMSC-derived SCLCs to phenotypically stable Schwann cells (hBMSC-dSCs). The effectiveness of hBMSC-dSC transplants for remyelination therapy after neurotrauma was demonstrated in rat models of sciatic nerve injury (SNI) and spinal cord contusion injury (SCI) (
Figure 1).
In the SNI model, rats treated with nerve conduits that had been seeded with hBMSC-dSCs showed significant improvement in gait 12 weeks post-injury compared to null in the vehicle control. In the SCI model, co-treatment with hBMSC-dSCs and chondroitinase ABC (ChABC, which digest axon-restrictive chondroitin sulphate (CS) enriched in the glial scar of injured CNS tissue [
7]) also resulted in significant improvement in motor function. The improvement in motor function was accompanied by robust axonal regrowth and remyelination, as observed in animals treated with hBMSC-dSCs. These results reinforce the use of hiSN in co-culture with hBMSC-derived SCLCs to provide for contact-mediated signaling towards fate-committed Schwann cells that promise translation into post-traumatic remyelination therapy.
2. Materials and Methods
2.1. Isolation and Culture of hBMSCs
Human bone marrow samples were collected from the iliac spine of 5 healthy donors with use of syringe aspiration under sterile conditions. The study protocol was approved by the Institutional Review Board of The University of Hong Kong/Hospital Authority Hong Kong West Cluster (Study No. UW 10-157).
Stock samples of hBMSC were established with slight modification of the method of Wolfe et al. [
16]. Briefly, bone marrow concentrate of each donor was diluted 5–10 times (as per sample quality) with BMSC medium, consisting of Minimum Essential Medium Eagle—Alpha Modification (MEM-alpha, Invitrogen, Waltham, MA, USA) with 15% (
v/
v) fetal bovine serum (FBS, French origin, Biosera, Nuaillé, France) and then plated onto a culture plate for maintenance in culture at 37 °C, 5% CO
2, and 95% humidity for 10–14 days with medium changed on alternate days to remove suspending hematopoietic cells. Human BMSCs were harvested using TrypLE™ Express (Gibco, Roskilde, Denmark) and plated onto low-adherence 6-well culture plates (Greiner, Frickenhausen, Germany) at 50,000 cells/cm
2 in BMSC medium. The cells were passaged at 1/3 density when they reached 80% confluence. Human BMSCs at passages 3 to 8 were sampled for immunocytochemical (ICC) assessment of markers CD73, CD90, CD105, and STRO-1.
2.2. Derivation of SCLCs from hBMSCs [5]
Briefly, hBMSCs in passages 3–6 were transferred to ultra-low-attachment 6-well culture plates (Corning, Corning, NY, USA) and cultured in sphere-forming medium (Neurobasal medium (NBM, Invitrogen, Carlsbad, CA, USA) supplemented with 2% B27 (Invitrogen, Carlsbad, CA,USA), 20 ng/mL epidermal growth factor (EGF, Peprotech, Rehovot, Israel), and 20 ng/mL basic fibroblast growth factor (bFGF, Peprotech, Rehovot, Israel). Neurosphere-like clusters emerged within 48 h and the cultures were maintained for 14 days. One third of the medium was replaced every 3 days with fresh sphere-forming medium that contained a triple concentration of supplements.
The hBMSC-derived neurospheres were transferred to 6-well culture plates (coated with laminin (Roche, Basel, Switzerland) and Poly-D-lysine (PDL, Sigma Aldrich, Saint Louis, MO, USA)) at 8–10 spheres/cm2, and cultured for 14 days in glia-induction medium (MEM-alpha medium supplemented with 10% FBS (South American origin, Biosera, France) and GIFs (5 μM forskolin (FSK, Sigma, St. Louis, MO, USA), 200 ng/mL β-heregulin (β-HRG, Peprotech, Rehovot, Israel), 5 ng/mL platelet-derived growth factor (PDGF)-AA (Peprotech, Rehovot, Israel), and 10 ng/mL bFGF)).
2.3. Derivation of Sensory Neurons (hiSNs) from hiPSCs [14]
Human iPSCs (IMR90) clone (#1) (WiCell Research Institute) was a gift from Prof. Elly S.W. Ngan (Department of Surgery, The University of Hong Kong). Briefly, hiPSCs were plated at a density of 1 × 105 cells/well on Matrigel-coated 6-well plates and cultured for 8 days in SN differentiation medium (DMEM/F12 medium supplemented with 10% KnockOut Serum Replacement (KSR, Gibco), 1% penicillin/streptomycin (P/S, Thermo Fisher), 0.3 μM Retinoic acid (Sigma), and small-molecule inhibitors cocktail (SMIs: 0.3 μM LDN-193189 (Cellagen Technology, San Diego, CA, USA), 2 μM A83-01 (Cellagen Technology, San Diego, CA, USA), 6 μM CHIR99021 (BioVision, CA, USA), 2 μM RO4929097 (Cellagen Technology, San Diego, CA, USA), and 3 μM SU5402 (Tocris, Bristol, UK)). The medium was refreshed on alternate days. Neuron morphology was checked 6–8 days after induction. At this point, the medium was changed to SN maintenance medium (NBM supplemented with 10 ng/mL neurotrophin 3 (NT-3, R&D Systems, Wiesbaden, Germany), 20 ng/mL brain-derived neurotrophic factor (BDNF, R&D Systems, Minneapolis, MN, USA), 20 ng/mL nerve growth factor (NGF, R&D Systems, Minneapolis, MN, USA), 20 ng/mL glial cell line-derived growth factor (GDNF, R&D Systems, Minneapolis, MN, USA), and 1% P/S. The cells were further cultured for 14 days.
2.4. Derivation of hBMSC-dSCs
The hBMSC-dSCLCs obtained were seeded (3000 cells/cm2) onto the hiSNs in culture and maintained in co-culture for 14 days in medium (MEM-alpha and NBM (1:1, v/v), supplemented with 5% FBS (South America origin), 1% (v/v) B27, 1% (v/v) P/S, 2.5 μM FSK, 2.5 ng/mL PDGF-AA, 5 ng/mL bFGF, 100 ng/mL β-HRG, and 5 ng/mL NGF). Half of the medium was replaced with fresh medium on alternate days. After 14 days, the cells were passaged to remove hiSNs. The culture was then maintained for at least 7 days in FBS-supplemented (10%) DMEM/F12 medium.
2.5. In Vitro Myelination Assay of hBMSC-dSC [11]
To test the myelinating power of the derived cells on primary neurons other than iSNs, DRG neurons were extracted from Sprague–Dawley (SD) rat embryos of day 14.5–15.5. Cells were dissociated from the DRGs by digestion with TrypLE Express for 15 min at 37 °C. The dissociated DRGs were seeded at a density of 2–3 DRGs/cm2 on PDL/laminin-coated round coverslips (14-mm diameter, Thermo Scientific) and maintained in DRG medium (NBM supplemented with 2% B27 (
v/
v) and 20 ng/mL NGF). After 48 h, the DRG medium was further supplemented with 10 μM fluorodeoxyuridine (Sigma, Saint Louis, MO, USA) and 10 μM uridine (Sigma, Saint Louis, MO, USA) for removing highly proliferative endogenous glial cells and fibroblasts until pure neuron culture was obtained. The purified DRG neuron culture was maintained for another 7 days in DRG medium. We then seeded hBMSC-dSCs at a density of 8000 cells/cm
2 onto the DRG neuronal network (
Figure 2A) and maintained it with y co-culture medium (MEM-alpha and NBM (1:1,
v/
v), supplemented with 10% FBS (South American origin), 2% B27, and 10 ng/mL NGF). The medium was refreshed on alternate days. Until the alignment of hBMSC-dSCs with neurites was observed, L-ascorbic acid (50 μg/mL, Sigma, Heidelberg. Germany) was added to induce myelination. Myelination was assessed after 14 days of co-culture.
2.6. Rat Model of Sciatic Nerve Injury
All surgical procedures in this study were in strict accordance with the National Institute of Health Guidelines for Laboratory Animal Care and Safety, and with approved protocols by the Committee on the Use of Live Animals and Teaching and Research, the University of Hong Kong. Adult male SD rats (220–250 g) were anaesthetized with ketamine/xylazine mixture (Alfasan, Woerden, Netherlands, intraperitoneal injection). The skin on the left thigh was incised to expose the left sciatic nerve. At the femur level, a 10 mm segment of nerve was excised. At the two ends, 2 mm of the stumps was telescoped into a 16 mm long chitosan conduit so that a 12 mm gap was kept between the stumps. The conduit was filled with (i) hBMSC-dSCs (2 × 105 cells in Matrigel) or (ii) Matrigel only (n = 4 each). Another group received reverse ligation with the excised fragment as a positive control (autograft). The conduit or autograft was secured with 7-O polydioxanone (PDS-II) monofilament suture (Ethicon). The wound was closed and the animals, after reviving in intensive care unit (Thermocare, CA), were returned to individual cages with food and water ad libitum for weeks with body weight monitored daily. Meloxicam (Boehringer Ingelheim, daily, in drinking water, one week after surgery), buprenorphine (Temgesic, Hull, UK, twice a day, subcutaneous injection, four days after surgery), oxytetracycline (Norbrook, Corby, UK, once every three days, subcutaneous injection, first week after surgery), and cyclosporin A (Selleckchem, immunosuppressant, 10 mg/kg, once per day, subcutaneous injection, throughout the holding period) were administered to the operated animals to suppress rejection.
2.7. Sciatic Functional Index (SFI)
The sciatic nerve provides motor input to the lower limbs, including the feet. One of the most measurable movements is the spread of toes, as controlled by the peroneal nerve, a distal branch of the sciatic nerve. Sciatic functional index, one of the gold standards for assessing functional recovery of sciatic nerve [
17,
18], was used. Functional recovery was monitored based on footprint analysis conducted every four weeks after surgery. An infrared walkway system was used for collecting the footprint data. The construction of the system was based on Fricker et al. [
19] with modifications (
Figure 3A). The footprints revealed on the acrylic board surface due to frustrated total internal reflection were recorded with infra-red camera with a resolution of 1920 × 1080 at 30 frames/second. For each animal, 3 footprints that were made during steady walking were assessed using ImageJ software (NIH).
Three parameters were measured (
Figure 3B): (i) the print length (PL, distance from the third toe to the heel), (ii) the toe spread (TS, distance from the 1st to the 5th toe), and (iii) the intermediate toe spread (ITS, distance from the 2nd to the 4th toe). The data obtained were used to compute the following three factors:
The SFI was calculated by adapting the above factors into the following formula [
17,
18]:
2.8. Electromyography
For measuring electromyography, the rats were anaesthetized with inhalation of isoflurane (1–2%) and kept warm at 37 °C. The sciatic nerve and gastrocnemius muscle on both sides were exposed. The intact nerves of the contralateral side were taken as the control. Double-hooked stainless steel stimulating electrode was used to suspend and stimulate the sciatic nerve at the proximal end (the end close to the spinal cord) or the distal end (the end close to gastrocnemius muscle), at 2 mm each from the graft. Two stainless-steel needle electrodes were inserted to gastrocnemius muscle for recording the compound muscle action potentials (CMAPs). The nerves were stimulated with single electrical pulses at supramaximal intensity. Similar procedures and measurements were made on the contralateral, intact side of each rat.
2.9. Rat Model of Spinal Cord Contusion
Adult male SD rats (220–250 g) were anesthetized by intraperitoneal injection of ketamine/xylazine cocktail (Alfasan). The spinal cord was exposed by dorsal laminectomy of the T9–T10 vertebrae. The contusion injury was induced by a weight-drop impactor device (W.M. Keck Center for Collaborative Neuroscience, Rutgers, State University of New Jersey). The animals were randomly assigned to two groups for contusive spinal cord injury, either moderate injury (dropping 10 g weight from 25 mm height) or severe injury (dropping 25 g weight from 50 mm height) [
20,
21]. To assess the success of contusion, we checked if (i) hemorrhage at the lesion site of the exposed spinal cord was observed immediately after contusion, and (ii) the BBB score at week one post-surgery was ≤2.
One of the following five treatments was administered to the operated rats: (i) Chondroitinase ABC (ChABC) injection followed by hBMSC-dSCs transplantation, (ii) ChABC injection followed by transplantation of human Schwann cells (hSCs, ScienCell, Catalogue #1700, passage 5 to 6), (iii) hBMSC-dSCs transplantation only, (iv) ChABC injection only, and (v) PBS injection only. ChABC was injected to the rostral and caudal edges of the lesion site. To each site, 50 mU ChABC (in 2 μL PBS) was injected. The grafted cells (hBMSC-dSCs or hSCs) were injected to 1 mm anterior and 1 mm posterior to the lesion site (for each injection, 2 × 105 cells in 2 μL MEM-alpha). The muscle and skin were sutured with 4-0 PDS-II suture and 4-0 nylon sutures, respectively. Post-operative care was similar to that for the sciatic-nerve-injured rat. Manual expression of urinary bladders was performed at least twice a day until recovery of the micturition reflex.
2.10. Basso, Beattie, and Bresnahan (BBB) Score Test
The BBB locomotor rating scale [
22] was used for the evaluation of locomotor recovery of rats with spinal cord injury. Hindlimb movement was scored from 0, indicating no observable spontaneous movement, to 21 points, indicating normal locomotor movements. Assessments were made weekly for up to 12 weeks post-operation by placing the rats individually onto a circular open field with a 100 cm diameter. The rats were encouraged to explore the open field for 4 min and the process was videotaped. The average score from two independent experimenters blinded to the experimental groups was considered the final score.
2.11. Ladder Walk Test
Before operation, the rats were exposed to the testing environment for training. At 12 weeks post-operation, the animals were encouraged to cross the ladder from a neutral cage to their home cage. The ladder was elevated at an angle of 55°. The whole process throughout the test was videotaped. The evaluation of hindlimb placement was performed according to the 7-category scaled foot fault scoring by Metz and Whishaw [
23].
2.12. Immunocytochemistry (ICC)/Immunohistochemistry (IHC)
For ICC, the cultures were fixed with 4% formaldehyde. hBMSC-derived neurosphere-like clusters were pelleted and resuspended in PBS and cytospun onto Superfrost slides (Thermo Scientific), followed by fixation with 4% formaldehyde. The cultures and cell clusters were then permeabilized and blocked in PBS with 0.1% Triton-X 100 and 3% bovine serum albumin (BSA).
For IHC, the rats were sacrificed at 12 weeks post-surgery and perfused with 0.9% saline followed by 4% formaldehyde. The sciatic nerves and spinal cords were extracted and post-fixed in formaldehyde for 2 h at room temperature. Fixed sciatic nerves and spinal cords were incubated in 30% sucrose at 4 °C for 4 days and then sectioned to 20 μm using CryoStar™ NX50 Cryostat (Thermo Scientific). The fixed sections were washed with PBS and then permeabilized and blocked in PBS with 0.7% Triton-X 100 and 3% BSA.
The samples were probed for CD73 (mouse, BD Pharmingen, 1:400), CD90 (mouse, BD Pharmingen, 1:500), CD105 (mouse, BD Pharmingen, 1:400), STRO-1 (mouse, R&D Systems, 1:400), nestin (mouse, R&D Systems, 1:400), CD45 (mouse, Millipore, 1:400), SOX2 (mouse, R&D Systems, 1:200), SOX10 (mouse, R&D Systems, 1:200), SSEA4 (mouse, DSHB, 1:400), Tuj1 (mouse, Biolegend, 1:400 (ICC) 1:200 (IHC); rabbit, Biolegend, 1:400 (ICC) 1:200 (IHC)), NF200 (NF200, rabbit, Sigma, 1:100), p75NTR (rabbit, Abcam, 1:500 (ICC) 1:200 (IHC)), myelin basic protein (MBP) (rabbit, Abcam, 1:200 (ICC); mouse, Millipore, 1:100 (IHC)), S100β (rabbit, Dako, ready-to-use), and human nucleus (mouse, Millipore, 1:50). Immunopositive signals were revealed with anti-mouse IgG Alex488/Alexa594 and anti-rabbit IgG Alex 488/Alexa 594 (Goat, Life Technologies, 1:400). The cell nuclei were counterstained with Hoechst 33,258 (Sigma). Fluorescent images were captured with LSM 800 Confocal microscope (Carl Zeiss).
2.13. Transmission Electron Microscopy
Formaldehyde-fixed tissues were trimmed into 1 mm cubes and sent to the Electron Microscope Unit, the University of Hong Kong, for further tissue processing. In brief, the tissue blocks were treated with cacodylate buffer with 0.1 M sucrose, then post-fixed in 1% osmium tetroxide for 1 h at room temperature. The tissue blocks were dehydrated in ethanol stepwise from 50% to 100% and then embedded in epoxy resin. The samples were sectioned to 75–90 nm thick with glass/diamond knife using ultra-microtome and then mounted on 150 mesh hexagonal copper grids. After staining with 2% aqueous uranyl acetate for 20 min, images were captured under a Philips CM100 transmission electron microscope.
2.14. Statistical Analysis
One-way analysis of variance (ANOVA) was used for the evaluation of significant differences between endpoint scores followed by Tukey’s post hoc test. Two-way ANOVA followed by Tukey’s post hoc test was used to evaluate the significant differences based on dpi and treatment group. Poisson regression (log–linear model) was used to evaluate the difference in foot fault scores. Differences were considered statistically significant with * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.
4. Discussion
In this study, we made use of hiSNs to mediate the commitment of hBMSC-SCLCs to the Schwann cell fate. The resultant hBMSC-dSCs were fate stable for multiple passages even after the withdrawal of GIFs and neurons. These hBMSC-dSCs were capable of myelinating axons both in vitro and in vivo. We further demonstrated the efficacy of promoting functional recovery following the transplantation of hBMSC-dSCs into sites of traumatic neural tissue injury as exemplified by sciatic nerve injury and spinal cord injury. Histological results revealed that these cells enwrapped regrowing axons with compact myelin in both PNS and CNS. Transplantation of hBMSC-dSCs led to significant improvement in motor function in the sciatic nerve injury model, reaching scores that approached those of autografts, the gold standard for bridging critical nerve gaps. When the restrictive CS moiety in the glial scar around the spinal cord lesions [
24,
26] was removed via ChABC activity, hBMSC-dSCs further demonstrated the capability of mediating axonal regrowth in the contused spinal cord; as a result, improvement in motor performance similar to that due to the transplantation of primary human Schwann cells was observed. Results open avenues for generating fate-committed Schwann cells using autologous cells that are effective in promoting functional recovery after trauma.
SCLCs can be derived from adult non-neural tissue [
4,
8] by enhancing signaling pathways such as integrin and bFGF signaling [
27]. However, such SCLCs suffer from phenotypic instability since the sequential activation of ErbB and Notch signaling pathways is required for the maturation of Schwann cells, and the stabilization of the Schwann cell phenotype is seldom addressed [
11,
12]. The direct addition of ErbB and Notch ligands to the medium is insufficient since the mechanical strain transmitted through the cell membrane also contributes to their activation. For this reason, contact with axons is the most efficient means to activate the signaling pathways that cement Schwann cell fate. In small animal models, rapid contact between transplanted SCLCs and axons is achieved due to small lesion size. In human patients with larger lesions, the regrowing axon fibers require time to reach the transplanted cells. This provides a higher chance for uncommitted SCLCs to spontaneously dedifferentiate into myofibroblasts. This not only reduces the number of functional Schwann cells to support therapeutic effects, but also causes fibrosis of the lesion site, further limiting rehabilitation.
In our pilot studies, rat DRG neurons were required to mediate the fate commitment of SCLCs obtained from hBMSC-derived neurospheres [
11]. However, the risk of animal cell contamination of human cell products renders such protocols unacceptable for clinical use. In this study, we present a rapid single-step protocol to obtain highly pure cultures of hiSN from iPSCs to present the suitable signaling ligands to hBMSC-derived SCLCs for their commitment to Schwann cell fate. The purity and functional capacity of such Schwann cells was not different from those generated via co-culture with rat DRG neurons [
12]. This was supported by immunocytochemical characterization with Schwann cell markers and demonstration of Schwann cell function by in vitro myelination (
Figure 2). Results open avenues for a completely autologous route towards obtaining fate-committed Schwann cells.
In sciatic nerve injury over a critical gap length, the inadequate recolonization of the lesion by supporting myelin-forming glial cells prevents spontaneous recovery [
6,
28]. Autograft and allograft, providing both physical guidance and Schwann cells to support regrowing axons, results in a high level of recovery. However, the clinical use of autografts is limited by donor site morbidity, and allografts are limited by availability and the need for immunosuppression to prevent host graft rejection. While immunosuppressants confer regenerative effect by limiting secondary injury caused by inflammatory response [
29], they cause numerous side effects such as muscle weakness [
30,
31] and increased risk of opportunistic infections. Using Schwann cells derived from autologous non-neuronal cells combined with synthetic scaffolds to provide physical guidance for the regrowth of axons across the lesion [
32] avoids these issues.
While endogenous Schwann cells can myelinate regrowing axons, their limited proliferative capacity and the time required for these cells to migrate into the scaffold results in insufficient recolonization of the gap within a defined time [
33]. The lack of supply of neurotrophic factors and low degree of myelination by these endogenous Schwann cells results in stunted regeneration that is unable as support functional recovery [
34,
35], as observed in the Matrigel-only group, where only a thin bundle of regenerated axons was found in the conduit. The seeding of Schwann cells to the synthetic scaffold prior to transplantation not only guaranteed an adequate supply of Schwann cells, but also circumvented the need for cells to migrate into the scaffold. When such scaffolds were applied to rats with sciatic nerve transection, regeneration at both the histological level and behavioral rehabilitation was comparable to autograft. The fasciculation of the regenerated sciatic nerve in rats that received hBMSC-dSC transplant in a manner similar to intact nerves further implied an advanced stage of regeneration. The advent of this easily accessible source of fate-committed and potentially autologous Schwann cells, combined with on-going efforts improve the physical guidance properties of the conduit [
36,
37], promises robust regeneration across nerve gaps.
The SFI assessment used in this study is regarded as the gold standard for evaluating the repair of the sciatic nerve [
17]. We further adopted ink-free computerized footprint analysis in this study (i) to reduce the anxiety of the animals when putting dye onto their feet and (ii) to avoid the smearing of footprints [
38]. The significant improvement in SFI after the transplantation of hBMSC-dSCs indicated the recovery of sciatic nerve function. This result is in line with larger diameter of the regenerating nerve, implying an increase in the number of fibers connecting the two ends. At 12 weeks post-injury, variation in the compact myelination of the regenerated nerves reduced propagation synchrony due to differences in conduction velocity, causing the broadening of CMAP and sub-optimal muscle activation. Nonetheless, this initial outcome highlights the prospect for hBMSC-dSCs to achieve the uniform myelination of the regenerating sciatic nerve if allowed a longer recovery time, resulting in the resynchronization of CMAP in the sciatic nerve and the restoration of motor function.
A similar reparative power of hBMSC-dSCs on CNS injury was revealed in the spinal cord contusion model. The apparent lack of recovery in the Schwann-cell-only groups (both hSC and hBMSC-dSC) was likely the result of the formation of an axon-restrictive CS-rich glial scar by astrocytes due to both injury and contact with Schwann cells [
24,
26]. Upon removal of this restrictive boundary via treatment with ChABC, axons ensheathed or myelinated by the grafted Schwann cells could be observed. The short observation period (12 weeks) limited the degree of functional recovery (in terms of BBB and foot fault scores), consistent with the findings of previous works by our group and other groups [
7,
25,
26]. Nonetheless, rats that received peri-lesional Schwann cell transplant and ChABC injection demonstrated improvements in recovery over those which received ChABC but without accompanying Schwann cell transplant. A longer observation time will reveal if further recovery could be achieved.