Mitochondrial Dysfunction in Lung Resident Mesenchymal Stem Cells from Idiopathic Pulmonary Fibrosis Patients
Abstract
:1. Introduction
2. Materials and Methods
2.1. Patient Recruitment
2.2. LRMSC Isolation and Validation
2.3. Transcriptomic Analysis
2.4. mRNA Analysis by qPCR
2.5. Mitochondrial Respiration Assay
2.6. Autophagy Assay
2.7. Electromicroscopy
2.8. TGFβ Treatments
2.9. Mitochondrial DNA Quantification
2.10. Mitochondrial Membrane Potential (MMP) Assessment
2.11. Statistical Analysis
3. Results
3.1. Oxidative Phosphorylation Is the Most Altered Pathway in LRMSC from IPF Patients
3.2. LRMSC from IPF Patients Present an Impaired Mitochondrial Respiration Capacity Compared to Control LRMSC
3.3. Mitochondrial Turnover Capacity Is Compromised in IPF LRMSC and Linked to Reduced Autophagy and Apoptosis
3.4. IPF and CON LRMSC Exhibit a Different Mitochondrial Response to TGFβ Exposure
4. Discussion and Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Number | Experimental Group | Cohort | Gender | Age |
---|---|---|---|---|
1 | IPF | Bellvitge | m | 65 |
2 | IPF | Bellvitge | f | 68 |
3 | IPF | Bellvitge | m | 74 |
4 | IPF | Bellvitge | m | 68 |
5 | IPF | Bellvitge | m | 62 |
6 | IPF | Bellvitge | m | 70 |
7 | IPF | Bellvitge | m | 66 |
8 | IPF | Lund | m | 68 |
9 | IPF | Lund | f | 62 |
10 | IPF | Lund | m | 67 |
11 | IPF | Lund | f | 65 |
12 | CON | HUSE | f | 39 |
13 | CON | HUSE | m | 77 |
14 | CON | HUSE | m | 75 |
15 | CON | HUSE | m | 62 |
16 | CON | HUSE | m | 65 |
17 | CON | HUSE | m | 70 |
18 | CON | HUSE | m | 57 |
19 | CON | HUSE | m | 77 |
20 | CON | Lund | m | 68 |
21 | CON | Lund | m | 43 |
22 | CON | Lund | m | 62 |
23 | CON | Lund | m | 39 |
24 | CON | Lund | f | 44 |
25 | CON | Lund | m | 62 |
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Mercader-Barceló, J.; Martín-Medina, A.; Truyols-Vives, J.; Escarrer-Garau, G.; Elowsson, L.; Montes-Worboys, A.; Río-Bocos, C.; Muncunill-Farreny, J.; Velasco-Roca, J.; Cederberg, A.; et al. Mitochondrial Dysfunction in Lung Resident Mesenchymal Stem Cells from Idiopathic Pulmonary Fibrosis Patients. Cells 2023, 12, 2084. https://doi.org/10.3390/cells12162084
Mercader-Barceló J, Martín-Medina A, Truyols-Vives J, Escarrer-Garau G, Elowsson L, Montes-Worboys A, Río-Bocos C, Muncunill-Farreny J, Velasco-Roca J, Cederberg A, et al. Mitochondrial Dysfunction in Lung Resident Mesenchymal Stem Cells from Idiopathic Pulmonary Fibrosis Patients. Cells. 2023; 12(16):2084. https://doi.org/10.3390/cells12162084
Chicago/Turabian StyleMercader-Barceló, Josep, Aina Martín-Medina, Joan Truyols-Vives, Gabriel Escarrer-Garau, Linda Elowsson, Ana Montes-Worboys, Carlos Río-Bocos, Josep Muncunill-Farreny, Julio Velasco-Roca, Anna Cederberg, and et al. 2023. "Mitochondrial Dysfunction in Lung Resident Mesenchymal Stem Cells from Idiopathic Pulmonary Fibrosis Patients" Cells 12, no. 16: 2084. https://doi.org/10.3390/cells12162084