Transcriptomic Signature of the Simulated Microgravity Response in Caenorhabditis elegans and Comparison to Spaceflight Experiments
Abstract
:1. Introduction
2. Materials and Methods
2.1. C. elegans Strain and Growth Conditions
2.2. RNA Isolation, Illumina Sequencing
2.3. Gene Expression Analysis
2.4. Functional Analysis of the Genes
2.5. Comparison of DEGs to the CERISE Four Days
2.6. Mass Spectrometry for Ceramides
2.7. Microgravity Simulation with Clinorotation
3. Results
3.1. Simulated Microgravity Triggers Differential Expression of Hundreds of Genes
3.2. Simulated Microgravity-Induced Gene Expression Differences Are Highly Maintained for Eight Days after Return to Ground Conditions
3.3. Longevity Regulating Pathways Are Affected under Simulated Microgravity
3.4. Sphingolipid Signaling Pathway Is Suppressed in Response to Simulated Microgravity
3.5. Identification of the Common Microgravity-Responsive Genes between Four-Day CERISE and Simulated Microgravity
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Çelen, İ.; Jayasinghe, A.; Doh, J.H.; Sabanayagam, C.R. Transcriptomic Signature of the Simulated Microgravity Response in Caenorhabditis elegans and Comparison to Spaceflight Experiments. Cells 2023, 12, 270. https://doi.org/10.3390/cells12020270
Çelen İ, Jayasinghe A, Doh JH, Sabanayagam CR. Transcriptomic Signature of the Simulated Microgravity Response in Caenorhabditis elegans and Comparison to Spaceflight Experiments. Cells. 2023; 12(2):270. https://doi.org/10.3390/cells12020270
Chicago/Turabian StyleÇelen, İrem, Aroshan Jayasinghe, Jung H. Doh, and Chandran R. Sabanayagam. 2023. "Transcriptomic Signature of the Simulated Microgravity Response in Caenorhabditis elegans and Comparison to Spaceflight Experiments" Cells 12, no. 2: 270. https://doi.org/10.3390/cells12020270